Small molecules facilitate rapid and synchronous iPSC generation

Nature Methods

Volumn 11, Issue 11, 2014, Pages 1170-1176

  Bar Nur, Ori ^a,b,c,d   Brumbaugh, Justin ^a,b,c,d   Verheul, Cassandra ^a,b,c,d   Apostolou, Effie ^a,b,c,d   Pruteanu Malinici, Iulian ^a,e,f   Walsh, Ryan M ^a,b,c,d   Ramaswamy, Sridhar ^a,b,e,f   Hochedlinger, Konrad ^a,b,c,d,e  

^a MASSACHUSETTS GENERAL HOSPITAL CANCER CENTER   (United States)

^b HARVARD STEM CELL INSTITUTE   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^d Department of Stem Cell and Regenerative Biology ^*  (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f BROAD INSTITUTE OF MIT AND HARVARD   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID; CHIR 99021; GLYCOGEN SYNTHASE KINASE 3 INHIBITOR; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR SOX2; UNCLASSIFIED DRUG; 6 [[2 [[4 (2,4 DICHLOROPHENYL) 5 (4 METHYL 1H IMIDAZOL 2 YL) 2 PYRIMIDINYL]AMINO]ETHYL]AMINO]NICOTINONITRILE; ANTIOXIDANT; ENHANCED GREEN FLUORESCENT PROTEIN; GLYCOGEN SYNTHASE KINASE 3; GLYCOGEN SYNTHASE KINASE 3 BETA; GREEN FLUORESCENT PROTEIN; KRUPPEL LIKE FACTOR; MYC PROTEIN, MOUSE; POU5F1 PROTEIN, MOUSE; PYRIDINE DERIVATIVE; PYRIMIDINE DERIVATIVE; SOX2 PROTEIN, MOUSE; TRANSCRIPTION FACTOR SOX;

ANIMAL CELL; APOPTOSIS; ARTICLE; ATTENUATION; CELL CULTURE; CELL CYCLE PHASE; CELL FATE; CELL MATURATION; CELL POPULATION; CELL PROLIFERATION; CELL TYPE; CHIMERA; CONTROLLED STUDY; MOLECULE; NONHUMAN; NUCLEAR REPROGRAMMING; NUCLEOTIDE SEQUENCE; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SOMATIC CELL; ANIMAL; ANTAGONISTS AND INHIBITORS; BIOSYNTHESIS; CELL CYCLE CHECKPOINT; CELL DIFFERENTIATION; CYTOLOGY; EMBRYONIC STEM CELL; GENETICS; INDUCED PLURIPOTENT STEM CELL; MOUSE;

ANIMALS; ANTIOXIDANTS; APOPTOSIS; ASCORBIC ACID; CELL CYCLE CHECKPOINTS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CELLULAR REPROGRAMMING; EMBRYONIC STEM CELLS; GLYCOGEN SYNTHASE KINASE 3; GREEN FLUORESCENT PROTEINS; INDUCED PLURIPOTENT STEM CELLS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; OCTAMER TRANSCRIPTION FACTOR-3; PROTO-ONCOGENE PROTEINS C-MYC; PYRIDINES; PYRIMIDINES; SOXB1 TRANSCRIPTION FACTORS;

EID: 84908574127     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3142     Document Type: Article

References (45)

1. Takahashi, K. &Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006
2. Yu, J, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-1920 (2007
3. Takahashi, K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007
4. Stadtfeld, M. &Hochedlinger, K. Induced pluripotency: history, mechanisms, and applications. Genes Dev. 24, 2239-2263 (2010
5. Eminli, S, et al. Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cells. Nat. Genet. 41, 968-976 (2009
6. Woltjen, K, et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766-770 (2009
7. Apostolou, E. &Hochedlinger, K. Chromatin Dynamics during cellular reprogramming. Nature 502, 462-471 (2013
8. Buganim, Y., Faddah, D.A. &Jaenisch, R. Mechanisms and models of somatic cell reprogramming. Nat. Rev. Genet. 14, 427-439 (2013
9. Papp, B. &Plath, K. Epigenetics of reprogramming to induced pluripotency. Cell 152, 1324-1343 (2013
10. Polo, J.M, et al. A molecular roadmap of reprogramming somatic cells into iPS cells. Cell 151, 1617-1632 (2012
11. Stadtfeld, M., Maherali, N., Breault, D.T. &Hochedlinger, K. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell 2, 230-240 (2008
12. Rais, Y, et al. Deterministic direct reprogramming of somatic cells to pluripotency. Nature 502, 65-70 (2013
13. Di Stefano, B, et al. C/EBPpoises B cells for rapid reprogramming into induced pluripotent stem cells. Nature 506, 235-239 (2014
14. dos Santos, R.L, et al. MBD3/NuRD facilitates induction of pluripotency in a context-dependent manner. Cell Stem Cell 15, 102-110 (2014
15. Stadtfeld, M., Maherali, N., Borkent, M. &Hochedlinger, K. A reprogrammable mouse strain from gene-Targeted embryonic stem cells. Nat. Methods 7, 53-55 10.1038/nmeth.1409 (2010
16. Feng, B., Ng, J.H., Heng, J.C. &Ng, H.H. Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells. Cell Stem Cell 4, 301-312 (2009
17. Stadtfeld, M, et al. Ascorbic acid prevents loss of Dlk1-Dio3 imprinting and facilitates generation of all-iPS cell mice from terminally differentiated B cells. Nat. Genet. 44, 398-405 (2012
18. Hou, P, et al. Pluripotent stem cells induced from mouse somatic cells by smallmolecule compounds. Science 341, 651-654 (2013
19. Federation, A.J., Bradner, J.E. &Meissner, A. The use of small molecules in somatic-cell reprogramming. Trends Cell Biol. 24, 179-187 (2014
20. Esteban, M.A, et al. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell 6, 71-79 (2010
21. Silva, J, et al. Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 6, e253 (2008
22. Chen, J, et al. Rational optimization of reprogramming culture conditions for the generation of induced pluripotent stem cells with ultra-high efficiency and fast kinetics. Cell Res. 21, 884-894 (2011
23. Guo, S, et al. Nonstochastic reprogramming from a privileged somatic cell state. Cell 156, 649-662 (2014
24. Hanna, J, et al. Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 462, 595-601 (2009
25. Kumar, R, et al. AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes. Nature 500, 89-92 (2013
26. Nakagawa, M, et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat. Biotechnol. 26, 101-106 (2008
27. Wernig, M., Meissner, A., Cassady, J.P. &Jaenisch, R. c-Myc is dispensable for direct reprogramming of mouse fibroblasts. Cell Stem Cell 2, 10-12 (2008
28. O'Malley, J, et al. High-resolution analysis with novel cell-surface markers identifies routes to iPS cells. Nature 499, 88-91 (2013
29. Blaschke, K, et al. Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells. Nature 500, 222-226 (2013
30. Monfort, A. &Wutz, A. Breathing-in epigenetic change with vitamin C. EMBO Rep. 14, 337-346 (2013
31. Chen, J, et al. Vitamin C modulates TET1 function During somatic cell reprogramming. Nat. Genet. 45, 1504-1509 (2013
32. Schwarz, B.A., Bar-Nur, O., Silva, J.C. &Hochedlinger, K. Nanog is dispensable for the generation of induced pluripotent stem cells. Curr. Biol. 24, 347-350 (2014
33. Stadtfeld, M, et al. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature 465, 175-181 (2010
34. Wray, J, et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat. Cell Biol. 13, 838-845 (2011
35. Ho, R., Papp, B., Hoffman, J.A., Merrill, B.J. &Plath, K. Stage-specific regulation of reprogramming to induced pluripotent stem cells by Wnt signaling and T cell factor proteins. Cell Rep. 3, 2113-2126 (2013
36. Yu, J, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797-801 (2009
37. Okita, K., Nakagawa, M., Hyenjong, H., Ichisaka, T. &Yamanaka, S. Generation of mouse induced pluripotent stem cells without viral vectors. Science 322, 949-953 (2008
38. Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G. &Hochedlinger, K. Induced pluripotent stem cells generated without viral integration. Science 322, 945-949 (2008
39. Vierbuchen, T, et al. Direct conversion of fibroblasts to functional neurons by defined factors. Nature 463, 1035-1041 (2010
40. Ieda, M, et al. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142, 375-386 (2010
41. Ladewig, J, et al. Small molecules enable highly efficient neuronal conversion of human fibroblasts. Nat. Methods 9, 575-578 (2012
42. Ravens, S, et al. MOF-Associated complexes have overlapping and unique roles in regulating pluripotency in embryonic stem cells and during differentiation. eLife 4, e02104 (2014
43. Schneider, C.A., Rasband, W.S. &Eliceiri, K.W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671-675 (2012
44. Eggan, K, et al. Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc. Natl. Acad. Sci. USA 98, 6209-6214 (2001
45. Schmittgen, T.D. &Livak, K.J. Analyzing real-Time PCR data by the comparative C(T) method. Nat. Protoc. 3, 1101-1108 (2008