Induced pluripotent stem cells generated without viral integration

Science

Volumn 322, Issue 5903, 2008, Pages 945-949

  Stadtfeld, Matthias ^a,b,d,e   Nagaya, Masaki ^c,e   Utikal, Jochen ^a,b,d,e   Weir, Gordon ^c,e   Hochedlinger, Konrad ^a,b,d,e  

^a MASSACHUSETTS GENERAL HOSPITAL CANCER CENTER   (United States)

^b HARVARD STEM CELL INSTITUTE   (United States)

^c JOSLIN DIABETES CENTER   (United States)

^d Massachusetts General Hospital   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOVIRUS VECTOR; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR SOX2;

DNA; GENE EXPRESSION; GENOME; HOMINID; METHYLATION; MUTATION; RODENT; VIRUS;

ANIMAL CELL; ARTICLE; DNA METHYLATION; FETUS; FIBROBLAST; GENE EXPRESSION; LIVER CELL; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ADENOVIRIDAE; ANIMALS; CELL DIFFERENTIATION; CELL LINE; CHIMERA; CLONING, MOLECULAR; FEMALE; FIBROBLASTS; GENES, MYC; GENETIC VECTORS; HEPATOCYTES; KRUPPEL-LIKE TRANSCRIPTION FACTORS; LIVER; MALE; MICE; MICE, SCID; NUCLEAR REPROGRAMMING; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS; PROTO-ONCOGENE PROTEINS C-MYC; SOXB1 TRANSCRIPTION FACTORS; TERATOMA; TRANSGENES; VIRUS INTEGRATION;

IPS; MUS;

EID: 55849115999     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1162494     Document Type: Article

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30. We thank H. Hock for critical comments on the manuscript and L. Prickett and K. Folz-Donahue for expert help with flow cytometry. We are especially grateful to J. Lee of the Harvard Gene Therapy Initiative for help with the generation of adenoviral vectors. M.S. was supported by a Schering postdoctoral fellowship, J.U. was supported by a Mildred Scheel postdoctoral fellowship and M.N. and G.W. received support from the Juvenile Diabetes Research Foundation. Support to K.H. was from the NIH Director's Innovator Award, the Harvard Stem Cell Institute, the Kimmel Foundation and the V Foundation. The authors are filing a patent based on the results reported in this paper.