PiggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

Nature

Volumn 458, Issue 7239, 2009, Pages 766-770

  Woltjen, Knut ^a   Michael, Iacovos P ^a,b   Mohseni, Paria ^a,b   Desai, Ridham ^a,b   Mileikovsky, Maria ^a   Hämäläinen, Riikka ^a   Cowling, Rebecca ^a   Wang, Wei ^c   Liu, Pentao ^c   Gertsenstein, Marina ^a   Kaji, Keisuke ^d   Sung, Hoon Ki ^a   Nagy, Andras ^a,b  

^a MOUNT SINAI HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^d UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BETA GALACTOSIDASE; DOXYCYCLINE; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; STAGE SPECIFIC EMBRYO ANTIGEN 1; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2; TRANSCRIPTION FACTOR;

CYTOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC MARKER;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL TRANSFORMATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; FIBROBLAST; GENE EXPRESSION; GENE SILENCING; GENE TRANSFER; GENETIC TRANSFECTION; GERM CELL; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; ONCOGENE C MYC; PIGGYBAC TRANSPOSITION; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOMATIC CELL; SOUTHERN BLOTTING; TRANSGENE; TRANSGENICS; TRANSPOSON; ANIMAL; CELL LINE; CYTOLOGY; GENE ORDER; GENE VECTOR; GENETICS; NUCLEAR REPROGRAMMING; NUDE MOUSE; PHYSIOLOGY; SEQUENCE ALIGNMENT; VIROLOGY;

IPS; MURINAE;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELLS, CULTURED; DNA TRANSPOSABLE ELEMENTS; FIBROBLASTS; GENE ORDER; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; MICE; MICE, NUDE; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELLS; SEQUENCE ALIGNMENT; TRANSCRIPTION FACTORS; TRANSGENES;

EID: 64749083939     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature07863     Document Type: Article

References (30)

1. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006).
2. Maherali, N. et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 1, 55-70 (2007).
3. Okita, K., Ichisaka, T. & Yamanaka, S. Generation of germline-competent induced pluripotent stem cells. Nature 448, 313-317 (2007).
4. Meissner, A., Wernig, M. & Jaenisch, R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nature Biotechnol. 25, 1177-1181 (2007).
5. Brambrink, T. et al., Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2, 151-159 (2008).
6. Stadtfeld, M., Nagaya, M., Utikal, J., Weir, G. & Hochedlinger, K. Induced pluripotent stem cells generated without viral integration. Science 322, 945-949 (2008).
7. Okita, K., Nakagawa, M., Hyenjong, H., Ichisaka, T. & Yamanaka, S. Generation of mouse induced pluripotent stem cells without viral vectors. Science 322, 949-953 (2008).
8. Ding, S. et al. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell 122, 473-483 (2005).
9. Wu, S. C. et al. piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Toi2, and Mos1 in mammalian cells. Proc. Natl Acad. Sci. USA 103, 15008-15013 (2006).
10. Cadinanos, J. & Bradley, A. Generation of an inducible and optimized piggyBac transposon system. Nucleic Acids Res. 35, e87 (2007).
11. Wang, W. et al. Chromosomal transposition of PiggyBac in mouse embryonic stem cells. Proc. Natl Acad. Sci. USA 105, 9290-9295 (2008).
12. Fraser, M. J., Ciszczon, T., Elick, T. & Bauser, C. Precise excision of TTAA-specific lepidopteran transposons piggyBac (IFP2) and tagalong (TFP3) from the baculovirus genome in cell lines from two species of Lepidoptera. Insect Mol. Biol. 5, 141-151 (1996).
13. Cary, L. C. et al. Transposon mutagenesis of baculoviruses: analysis of Trichoplusia nl transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology 172, 156-169 (1989).
14. Kaji, K. et al. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature doi:101038/nature07864 (this issue).
15. Ellis, J. Silencing and variegation of gammaretrovirus and lentivirus vectors. Hum. Gene Ther. 16, 1241-1246 (2005).
16. Agha-Mohammadi, S. et al. Second-generation tetracycline- reguiatable promoter: repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness. J. Gene Med. 6, 817-828 (2004).
17. Belteki, G. et al. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res. 33, e51 (2005).
18. Nagy, A., Rossant, J., Nagy, R., Abramow-Neweriy, W. & Roder, J. C. Derivation of completely cell culture-derived mice from early-passage embryonic stem cells. Proc. Natl Acad. Sci. USA 90, 8424-8428 (1993).
19. Stadtfeld, M., Maherali, N., Breault, D. T. & Hochedlinger, K. Defining molecular cornerstones during fibroblast to iPS ceil reprogramming in mouse. Cell Stem Cell 2, 230-240 (2008).
20. Kaji, K. et al. The NuRD component Mbd3 is required for pluripotency of embryonic stem cells. Nature Cell Biol. 8, 285-292 (2006).
21. Kim, J. B. et al. Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature 454, 646-650 (2008).
22. Mikkelsen, T. S. et al. Dissecting direct reprogramming through integrative genomic analysis. Nature 454, 49-55 (2008).
23. Fujiwara, Y. et al. Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage. Proc. Nail Acad. Sci. USA 91, 12258-12262 (1994).
24. Huangfu, D. et al. Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nature Biotechnol. 26, 1269-1275 (2008).
25. Wernig, M. et al. A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nature Biotechnol. 26, 916-924 (2008).
26. Takahashi, K., Okita, K., Nakagawa, M. & Yamanaka, S. Induction of pluripotent stem cells from fibroblast cultures. Nature Protocols 2, 3081-3089 (2007).
27. Dafa'alla, T. H. et al. Transposon-free insertions for insect genetic engineering. Nature Biotechnol. 24, 820-821 (2006).
28. Wilson, M. H., Coates, C. J. & George, A. L. Jr. PiggyBac transposon-mediated gene transfer in human cells. Mol. Ther. 15, 139-145 (2007).
29. Mitra, R., Fain-Thornton, J. & Craig, N. L. piggyBac can bypass DNA synthesis during cut and paste transposition. EMBOJ. 27, 1097-1109 (2008).
30. Nagy, A. Manipulating the Mouse Embryo: A Laboratory Manual 3rd edn (Cold Spring Harbor Laboratory Press, 2003).