Generation of mouse induced pluripotent stem cells without viral vectors

Science

Volumn 322, Issue 5903, 2008, Pages 949-953

  Okita, Keisuke ^a   Nakagawa, Masato ^a,b   Hyenjong, Hong ^b   Ichisaka, Tomoko ^a,c   Yamanaka, Shinya ^a,b,c,d  

^a KYOTO UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d GLADSTONE INSTITUTE OF CARDIOVASCULAR DISEASE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR SOX2;

DNA; DRUG; EMBRYO; GENE EXPRESSION; GENOME; HOMINID; PLASMID; RODENT; TUMOR; VIRUS;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL REGENERATION; EMBRYO; FIBROBLAST; MOUSE; NONHUMAN; PLASMID; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; CHIMERA; DNA, COMPLEMENTARY; EMBRYO, MAMMALIAN; EMBRYONIC STEM CELLS; FIBROBLASTS; GENES, MYC; GENETIC VECTORS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; NUCLEAR REPROGRAMMING; OCTAMER TRANSCRIPTION FACTOR-3; PLASMIDS; PLURIPOTENT STEM CELLS; RETROVIRIDAE; SOXB1 TRANSCRIPTION FACTORS; TERATOMA; TRANSFECTION;

IPS; MUS;

EID: 55849117368     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1164270     Document Type: Article

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21. We thank K. Takahashi, K. Yae, M. Koyanagi, and K. Tanabe for scientific discussion; K. Okuda, M. Narita, A. Okada, H. Miyachi, S. Kitano, and N. Takizawa for technical assistance; R. Kato, R. Iyama, E. Nishikawa, and Y. Shimazu for administrative assistance; and D. Srivastava for critical reading of the manuscript. We also thank J. Miyazaki for the CAG promoter, T. Kitamura for Plat-E cells and pMXs retroviral vectors, and R. Farese for RF8 ES cells. This study was supported in part by a grant from the Program for Promotion of Fundamental Studies in Health Sciences of National Institute of Biomedical Innovation (NIBIO), a grant from the Leading Project of Ministry of Education, Culture, Sports, Science, and Technology (MEXT), a grant from Uehara Memorial Foundation, and grants-in-aid for scientific research of Japan Society for the Promotion of Science (JSPS) and MEXT (to S.Y.). K.O. was a JSPS research fellow. H.H. is supported by a Japanese government (MEXT) scholarship. The authors are filing a patent based on the results reported in this paper.