The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats

Experimental and Toxicologic Pathology

Volumn 66, Issue 9-10, 2014, Pages 407-413

  Boyacioglu, Murat ^a   Sekkin, Selim ^a   Kum, Cavit ^a   Korkmaz, Deniz ^a   Kiral, Funda ^a   Yalinkilinc, Hande Sultan ^a   Ak, Mehmet Onur ^a   Akar, Ferda ^a  

^a ADNAN MENDERES UNIVERSITY   (Turkey)

Author keywords

Comet assay; Hyperhomocysteinemia; Lymphocyt; Oxidative stress; Rats; Vitamin C

Indexed keywords

ASCORBIC ACID; CATALASE; DNA; GLUTATHIONE; HOMOCYSTEINE; MALONALDEHYDE; METHIONINE; SUPEROXIDE DISMUTASE; ANTIOXIDANT;

AMINO ACID BLOOD LEVEL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; CHRONIC DISEASE; CONTROLLED STUDY; DNA DAMAGE; DRUG EFFICACY; ENZYME ACTIVITY; HEART; HISTOPATHOLOGY; HYPERHOMOCYSTEINEMIA; KIDNEY PARENCHYMA; LIVER; LYMPHOCYTE; MALE; NONHUMAN; OXIDATIVE STRESS; RAT; THORACIC AORTA; ANIMAL; AORTA; CHEMICALLY INDUCED; COMET ASSAY; DISEASE MODEL; DRUG EFFECTS; PATHOLOGY; WISTAR RAT;

ANIMALS; ANTIOXIDANTS; AORTA; ASCORBIC ACID; COMET ASSAY; DISEASE MODELS, ANIMAL; DNA DAMAGE; HYPERHOMOCYSTEINEMIA; MALE; OXIDATIVE STRESS; RATS; RATS, WISTAR;

EID: 84908478075     PISSN: 09402993     EISSN: 16181433     Source Type: Journal    
DOI: 10.1016/j.etp.2014.06.004     Document Type: Article

References (62)

1. Abais J.M., Xia M., Li G., Gehr T.W., Boini K.M., Li P.L. Contribution of endogenously produced reactive oxygen species to the activation of podocyte NLRP3 inflammasomes in hyperhomocysteinemia. Free Radic Biol Med 2014, 67:211-220.
2. Anderson D., Yu T.W., McGregor D.B. Comet assay responses as indicators of carcinogen exposure. Mutagenesis 1998, 136:539-555.
3. Aydin S., Tokac M., Taner G., Arikok A.T., Dundar H.Z., Ozkardes A.B., et al. Antioxidant and antigenotoxic effects of lycopene in obstructive jaundice. J Surg Res 2013, 182:285-295.
4. Bagi Z., Cseko C., Toth E., Koller A. Oxidative stress-induced dysregulation of arteriolar wall shear stress and blood pressure in hyperhomocysteinemia is prevented by chronic vitamin C treatment. Am J Physiol Heart Circ Physiol 2003, 285:2277-2283.
5. Bergmeyer H., Gawehn K., Grasse M. Enzyme as biochemical reagents. Methods of enzyme analysis 1974, 438-458. Academic Press, New York. H.V. Bergmeyer (Ed.).
6. Cascalheira J.F., Parreira M.C., Viegas A.N., Faria M.C., Domingues F.C. Serum homocysteine: relationship with circulating levels of cortisol and ascorbate. Ann Nutr Metab 2008, 53:67-74.
7. Collins A.R., Dobson V.L., Dusinska M., Kennedy G., Stetina R. The comet assay: what can it really tell us?. Mutat Res-Fundam Mol Mech 1997, 375:183-193.
8. Conover W.J. Practical nonparametric statistics some methods based on ranks several independent samples 1980, 229-239. John Wiley & Sons, New York. 2nd ed.
9. Crossman G.A. A modification of Mallory's connective tissue stain with a discussion of the principles involved. Anat Rec 1937, 69:33-38.
10. De Bree A., Verschuren W.M.M., Kromhout D., Kluijtmans L.A.J., Blom H.J. Homocysteine determinants and the evidence to what extent homocysteine determines the risk of coronary heart disease. Pharmacol Rev 2002, 54:599-618.
11. Deicher R., Ziai F., Bieglmayer C., Schillinger M., Horl W.H. Low total vitamin C plasma level is a risk factor for cardiovascular morbidity and mortality in hemodialysis patients. J Am Soc Nephrol 2005, 16:1811-1818.
12. Du J., Cullen J.J., Buettner G.R. Ascorbic acid: chemistry, biology and the treatment of cancer. BBA-Rev Cancer 2012, 1826:443-457.
13. Duarte T.L., Lunec J. Review: when is an antioxidant not an antioxidant? A review of novel actions and reactions of vitamin C. Free Radic Res 2005, 39:671-686.
14. Fairbairn D.W., Olive P.L., O'Neill K.L. The comet assay: a comprehensive review. Mutat Res Genet Toxicol 1995, 339:37-59.
15. Ferrari C.K.B. Functional foods and physical activities in health promotion of aging people. Maturitas 2007, 58:327-339.
16. Filip C., Elena A., Nina Z., Irina J.M., Mihaela S., Luminita J., et al. Hyperhomocysteinemia's effect on antioxidant capacity in rats. Cent Eur J Med 2010, 5:620-626.
17. Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem 1995, 64:97-112.
18. Ghezzi P., Bonetto V., Fratelli M. Thiol-disulfide balance: from the concept of oxidative stress to that of redox regulation. Antioxid Redox Signal 2005, 7:964-972.
19. Hempel L.S. Homocysteine decreases endothelial cell glutathione, increasing the sensitivity to oxidants. Circulation 1998, 98:386.
20. Higashi Y., Noma K., Yoshizumi M., Kihara Y. Endothelial function and oxidative stress in cardiovascular diseases. Circ J 2009, 73:411-418.
21. Imlay J.A. Pathways of oxidative damage. Annu Rev Microbiol 2003, 57:395-418.
22. Jiang Y., Zhang H., Sun T., Wang J., Sun W., Gong H., et al. The comprehensive effects of hyperlipidemia and hyperhomocysteinemia on pathogenesis of atherosclerosis and DNA hypomethylation in ApoE-/- mice. Acta Biochim Biophys Sin 2012, 44:866-875.
23. Kahl V.F., Reyes J.M., Sarmento M.S., da Silva J. Mitigation by vitamin C of the genotoxic effects of nicotine in mice, assessed by the comet assay and micronucleus induction. Mutat Res-Genet Toxicol Env 2012, 744:140-144.
24. Kolling J., Scherer E.B., da Cunha A.A., da Cunha M., Wyse A.T. Homocysteine induces oxidative-nitrative stress in heart of rats: prevention by folic acid. Cardiovasc Toxicol 2011, 11:67-73.
25. Krobock E., Rahbari H., Mehregan A.H. Acid orcein and Giemsa stain modification of a valuable stain for dermatologic specimens. J Cutan Pathol 1978, 5:37-38.
26. 12 serum levels. Ann Neurol 2011, 69:352-359.
27. Liu D.S., Gao W., Liang E.S., Wang S.L., Lin W.W., Zhang W.D., et al. Effects of allicin on hyperhomocysteinemia-induced experimental vascular endothelial dysfunction. Eur J Pharmacol 2013, 714:163-169.
28. +-ATPase activity and oxidative status in rats hippocampus: protection by vitamin C. Metab Brain Dis 2011, 26:61-67.
29. 12 deficiency and hyperhomocysteinemia as correlates of cardiovascular risk factors in Indian subjects with coronary artery disease. J Cardiol 2013, 61:289-294.
30. Malinowska J., Kolodziejczyk J., Olas B. The disturbance of hemostasis induced by hyperhomocysteinemia: the role of antioxidants. Acta Biochim Pol 2012, 59:185-194.
31. Matte C., Mackedanz V., Stefanello F.M., Scherer E.B.S., Andreazza A.C., Zanotto C., et al. Chronic hyperhomocysteinemia alters antioxidant defenses and increases DNA damage in brain and blood of rats: protective effect of folic acid. Neurochem Int 2009, 54:7-13.
32. Mendes R.H., Mostarda C., Candido G.O., Moraes-Silva I.C., D'Almeida V., Belló-Klein A., et al. Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats. Auton Neurosci-Basic 2014, 180:43-47.
33. Murawska-Cialowicz E., Januszewska L., Zuwala-Jagiello J., Milczarska J., Zawadzki M., Paprocka-Borowicz M., et al. Melatonin decreases homocysteine level in blood of rats. J Physiol Pharmacol 2008, 259:717-729.
34. Noll C., Hamelet J., Ducros V., Belin N., Paul J.L., Delabar J.M., et al. Resveratrol supplementation worsen the dysregulation of genes involved in hepatic lipid homeostasis observed in hyperhomocysteinemic mice. Food Chem Toxicol 2009, 47:230-236.
35. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp Cell Res 1992, 198:259-267.
36. Osto E., Cosentino F. The role of oxidative stress in endothelial dysfunction and vascular inflammation. Nitric Oxide-Biol Chem 2010, 2:705-754.
37. Padayatty S.J., Katz A., Wang Y., Eck P., Kwon O., Lee J.H., et al. Vitamin C as an antioxidant: evaluation of its role in disease prevention. J Am Coll Nutr 2003, 22:18-35.
38. Pexa A., Herrmann M., Taban-Shomal O., Henle T., Deussen A. Experimental hyperhomocysteinaemia: differences in tissue metabolites between homocystine and methionine feding in a rat model. Acta Physiol 2009, 197:27-34.
39. Picerno I., Chirico C., Condello S., Visalli G., Ferlazzo N., Gorgone G., et al. Homocysteine induces DNA damage and alterations in proliferative capacity of T-lymphocytes: a model for immunosenescence. Biogerontology 2007, 8:111-119.
40. Pinkus H., Hunter R. Simplified acid orcein and Giemsa technique for routine staining of skin sections. Arch Dermatol 1960, 82:699-700.
41. Pravenec M., Kozich V., Krijt J., Sokolova J., Zidek V., Landa V., et al. Folate deficiency is associated with oxidative stress, increased blood pressure, and insulin resistance in spontaneously hypertensive rats. Am J Hypertens 2013, 26:135-140.
42. Robin S., Courdeot-Masuyer C., Nicod L., Jacqueson A., Richert L., Berthelot A. Opposite effect of methionine supplemented diet, a model of hyperhomocysteinemia, on plasma and liver antioxidant status in normotensive and spontaneously hypertensive rats. J Nutr Biochem 2004, 15:80-89.
43. Sauls D.L., Boyd L.C., Allen J.C., Hoffman M. Differences in the metabolic response to exogenous homocysteine in juvenile and adult rabbits. J Nutr Biochem 2004, 15:96-102.
44. Selhub J. Homocysteine metabolism. Annu Rev Nutr 1999, 19:217-246.
45. Seo H., Oh H., Park H., Jang Y., Lee M. Contribution of dietary of antioxidants to homocysteine-induced low density lipoprotein (LDL) oxidation in atherosclerotic patients. Yonsei Med J 2010, 51:526-533.
46. Sharma S., Singh M., Sharma P.L. Mechanism of hyperhomocysteinemia-induced vascular endothelium dysfunction-possible dysregulation of phosphatidylinositol-3-kinase and its downstream phosphoinositide dependent kinase and protein kinase B. Eur J Pharmacol 2013, 721:365-372.
47. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988, 175:184-191.
48. Suhail N., Bilal N., Khan H.Y., Hasan S., Sharma S., Khan F., et al. Effect of vitamins C and E on antioxidant status of breast-cancer patients undergoing chemotherapy. J Clin Pharm Ther 2012, 37:22-26.
49. Sun Y., Oberley L.W., Li Y. A simple for clinical assay of superoxide dismutase. Clin Chem 1988, 34:497-500.
50. Szeto Y.T., To T.L., Pak S.C., Kalle W. A study of DNA protective effect of orange juice supplementation. Appl Physiol Nutr Metab 2013, 38:533-536.
51. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000, 35:206-221.
52. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Ann Clin Biochem 1969, 27:502-522.
53. Ueland P.M., Refsum H., Beresford S.A., Vollset S.E. The controversy over homocysteine and cardiovascular risk. Am J Clin Nutr 2000, 72:324-332.
54. Ungvari Z., Pacher P., Rischak K., Szollar L., Koller A. Dysfunction of nitric oxide mediation in isolated rat arterioles with methionine diet-induced hyperhomocysteinemia. Arterioscler Thromb Vasc 1999, 19:1899-1904.
55. Valko M., Izakovic M., Mazur M., Rhodes C.J., Telser J. Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 2004, 266:37-56.
56. Weiss N. Mechanisms of increased vascular oxidant stress in hyperhomocysteinemia and its impact on endothelial function. Curr Drug Metab 2005, 6:27-36.
57. Verhoef P., Kok F.J., Kruyssen D.A.C.M., Schouten E.G., Witteman J.C.M., Grobbee D.E., et al. Plasma total homocysteine, B vitamins, of coronary atherosclerosis. Arterioscler Thromb Vasc Biol 1997, 17:989-995.
58. Victor V., Rocha M., Sola E., Banuls C., Garcia-Malpartida K., Hernandez-Mijares A. Oxidative stress, endothelial dysfunction and atherosclerosis. Curr Pharm Des 2009, 15:2988-3002.
59. Wiseman H., Halliwell B. Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem J 1996, 313:17-29.
60. Yamada H., Akahoshi N., Kamata S., Hagiya Y., Hishiki T., Nagahata Y., et al. Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine γ-lyase, an animal model of cystathioninuria. Free Radic Biol Med 2012, 52:1716-1726.
61. Yang R.X., Huang S.Y., Yan F.F., Lu X.T., Xing Y.F., Liu Y., et al. Danshensu protects vascular endothelia in a rat model of hyperhomocysteinemia. Acta Pharmacol Sin 2010, 31:1395-1400.
62. Yoshioka T., Kawada K., Shimada T., Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 1979, 135:372-376.