Single-molecule pull-down (SiMPull) for new-age biochemistry: Methodology and biochemical applications of single-molecule pull-down (SiMPull) for probing biomolecular interactions in crude cell extracts

BioEssays

Volumn 36, Issue 11, 2014, Pages 1109-1119

  Aggarwal, Vasudha ^a   Ha, Taekjip ^a,b,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b University of Illinois at Urbana Champaign   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Co immunoprecipitation; Fluorescence; Protein complex; Pull down; Single molecule; Western blotting

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANTIBODY; CARBOHYDRATE; ENZYME; IMMUNOGLOBULIN; NANOBODY; PROTEIN; CELL EXTRACT; MULTIPROTEIN COMPLEX;

ARTICLE; BIOCHEMISTRY; BIOCOMPATIBILITY; BIOLOGICAL PHENOMENA AND FUNCTIONS CONCERNING THE ENTIRE ORGANISM; BIOSENSOR; COMPLEX FORMATION; CYTOSOL; ENZYME LINKED IMMUNOSORBENT ASSAY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GENETIC PROCEDURES; HUMAN; IMMUNOPRECIPITATION; INTERMETHOD COMPARISON; MASS SPECTROMETRY; MEMBRANE; METHODOLOGY; MICROFLUIDICS; MOLECULE; OLIGOMERIZATION; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SINGLE MOLECULE PULL DOWN; STOICHIOMETRY; SURFACE PLASMON RESONANCE; TECHNOLOGY; WESTERN BLOTTING; CELL MEMBRANE; GENE DOSAGE; METABOLISM; PROCEDURES; PROTEIN ANALYSIS;

BIOCHEMISTRY; CELL EXTRACTS; CELL MEMBRANE; CYTOSOL; GENE DOSAGE; MICROSCOPY, FLUORESCENCE; MULTIPROTEIN COMPLEXES; PROTEIN INTERACTION MAPPING; PROTEIN INTERACTION MAPS;

EID: 84907882014     PISSN: 02659247     EISSN: 15211878     Source Type: Journal    
DOI: 10.1002/bies.201400090     Document Type: Article

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