Production of recombinant Chikungunya virus envelope 2 protein in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 98, Issue 6, 2014, Pages 2461-2471

  Tripathi, Nagesh K ^a   Priya, Raj ^a   Shrivastava, Ambuj ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

Author keywords

Bioreactor; Chikungunya virus; Cultivation; ELISA; Escherichia coli

Indexed keywords

AFFINITY CHROMATOGRAPHY; BATCH DATA PROCESSING; BIOREACTORS; COST EFFECTIVENESS; CULTIVATION; DIAGNOSIS; ESCHERICHIA COLI; PROTEINS; PURIFICATION; UREA; VIRUSES;

CHIKUNGUNYA; CULTIVATION CONDITIONS; ELISA; ENZYME LINKED IMMUNOSORBENT ASSAY; FED-BATCH CULTIVATION; HIGH-CELL-DENSITY CULTIVATIONS; LARGE SCALE PRODUCTIONS; SHAKE-FLASK CULTURES;

RECOMBINANT PROTEINS;

RECOMBINANT CHIKUNGUNYA VIRUS ENVELOPE 2 PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN;

BIOLOGICAL PRODUCTION; BIOREACTOR; CHIKUNGUNYA; COLIFORM BACTERIUM; GENETIC ENGINEERING; IMMUNOASSAY; PROTEIN; RECOMBINATION; VIRUS;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIUM ISOLATION; BATCH PROCESS; BLOOD SAMPLING; CELL DENSITY; CELL GROWTH; CELL INCLUSION; CHIKUNGUNYA; CHIKUNGUNYA VIRUS; COST EFFECTIVENESS ANALYSIS; CYTOLYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; ESCHERICHIA COLI; FED BATCH FERMENTATION; HUMAN; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LARGE SCALE PRODUCTION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; WESTERN BLOTTING;

AFRICA; ASIA;

CHIKUNGUNYA VIRUS; ESCHERICHIA COLI;

CHIKUNGUNYA VIRUS; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA; ESCHERICHIA COLI; INCLUSION BODIES; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION; VIRAL ENVELOPE PROTEINS;

EID: 84907849411     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5426-4     Document Type: Article

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