Development of a pilot-scale production process and characterization of a recombinant Japanese encephalitis virus envelope domain III protein expressed in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 95, Issue 5, 2012, Pages 1179-1189

  Tripathi, Nagesh K ^a,b   Shrivastava, Ambuj ^a   Biswal, Karttik C ^b   Rao, P V Lakshmana ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^b NATIONAL INSTITUTE OF TECHNOLOGY   (India)

Author keywords

Immunogenicity; Japanese encephalitis vaccine; Pilot scale fermentation; Purification; Scale up

Indexed keywords

IMMUNOGENICITY; ION-EXCHANGE CHROMATOGRAPHY; JAPANESE ENCEPHALITIS VIRUS; JAPANESE ENCEPHALITIS VIRUSES (JEV); NEUTRALIZING ANTIBODIES; PILOT SCALE; PILOT-SCALE PRODUCTION; SCALE-UP;

AFFINITY CHROMATOGRAPHY; ANTIBODIES; ESCHERICHIA COLI; FERMENTATION; PURIFICATION; UREA; VACCINES; VIRUSES;

RECOMBINANT PROTEINS;

IMMUNOLOGICAL ADJUVANT; JAPANESE ENCEPHALITIS VACCINE; JAPANESE ENCEPHALITIS VIRUS ENVELOPE DOMAIN III PROTEIN; NEUTRALIZING ANTIBODY; UNCLASSIFIED DRUG; RECOMBINANT PROTEIN; RECOMBINANT VACCINE; SUBUNIT VACCINE; VIRUS ANTIBODY; VIRUS ENVELOPE PROTEIN; VIRUS VACCINE;

ANTIBODY; BIOACTIVITY; EXPERIMENTAL STUDY; FERMENTATION; GENE EXPRESSION; GLYPHOSATE; MICROBIAL ACTIVITY; PROTEIN; VIRUS;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ARTICLE; BATCH FERMENTATION; BIOMASS; CELL INCLUSION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMORAL IMMUNITY; ION EXCHANGE CHROMATOGRAPHY; JAPANESE ENCEPHALITIS VIRUS; MOUSE; NONHUMAN; PH; PROTEIN ANALYSIS; PROTEIN EXPRESSION; TEMPERATURE; VERO CELL; WESTERN BLOTTING; ANIMAL; BIOTECHNOLOGY; BLOOD; FERMENTATION; GENE EXPRESSION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY; SERODIAGNOSIS;

ESCHERICHIA COLI; JAPANESE ENCEPHALITIS VIRUS; MUS;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIBODIES, VIRAL; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; ENCEPHALITIS VIRUS, JAPANESE; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; MICE; NEUTRALIZATION TESTS; RECOMBINANT PROTEINS; VACCINES, SUBUNIT; VACCINES, SYNTHETIC; VIRAL ENVELOPE PROTEINS; VIRAL VACCINES;

EID: 84875810296     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4100-6     Document Type: Article

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