Production and single-step purification of Brugia malayi abundant larval transcript (ALT-2) using hydrophobic interaction chromatography

Journal of Industrial Microbiology and Biotechnology

Volumn 37, Issue 10, 2010, Pages 1053-1059

  Bhuvanesh, Surianarayanan ^a   Arunkumar, Chakkaravarthi ^a   Kaliraj, Perumal ^a   Ramalingam, Subramanian ^a  

^a ANNA UNIVERSITY   (India)

Author keywords

Abundant larval transcript (ALT 2); DO stat; Fed batch; Hydrophobic interaction chromatography (HIC); Lymphatic filariasis; Recombinant vaccine antigen

Indexed keywords

ABUNDANT LARVAL TRANSCRIPT 2; DISSOLVED OXYGEN; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOMASS; BRUGIA MALAYI; CYTOPLASM; ESCHERICHIA COLI; FED BATCH FERMENTATION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PROTEIN PURIFICATION; REACTOR;

ANTIGENS, HELMINTH; BIOTECHNOLOGY; CHROMATOGRAPHY, LIQUID; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION; UP-REGULATION;

BACTERIA (MICROORGANISMS); BRUGIA MALAYI; ESCHERICHIA COLI; LURIA;

EID: 78149361260     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-010-0753-2     Document Type: Article

References (30)

1. Anand SB, Murugan V, Prabhu PR, Anandharaman V, Reddy MV, Kaliraj P (2008) Comparison of immunogenicity, protective efficacy of single and cocktail DNA vaccine of Brugia malayi abundant larval transcript (ALT-2) and thioredoxin peroxidase (TPX) in mice. Acta Trop 107(2):106-112
2. Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 10(5):411-421
3. Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93-99
4. Chen Q, Bentley WE, Weigand WA (1995) Optimization for a recombinant E. coli fed-batch fermentation. Appl Biochem Biotech 51(52):449-461
5. Cserjan-Puschmann M, Kramer W, Duerrschmid E, Striedner G, Bayer K (1999) Metabolic approaches for the optimisation of recombinant fermentation processes. Appl Microbiol Biotechnol 53:43-50
6. Geng X, Chang X (1992) High-performance hydrophobic interaction chromatography as a tool for protein refolding. J Chromatogr 599:185-194
7. Geng X, Bai Q, Zhang Y, Li X, Wu D (2004) Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography. J Biotechnol 113:137-149
8. Gnanasekar M, Rao KVN, He Y-X, Mishra PK, Nutman TB, Kaliraj P, Ramaswamy K (2004) Novel phage display-based subtractive screening to identify vaccine candidates of Brugia malayi. Infect Immun 4707-4715. doi: 10.1128/IAI.72.8.4707- 4715.2004
9. Gregory WF, Atmadja AK, Allen JE, Maizels RM (2000) The abundant larval transcript-1 and -2 genes of Brugia malayi encode stage-specific candidate vaccine antigens for filariasis. Infect Immun 4174-4179
10. Harlow E, David L (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
11. Hettwer S, Sterner R (2002) A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role. J Biol Chem 277(10):8194-8201
12. Jenney FE Jr, Adams MW (2008) The impact of extremophiles on structural genomics (and vice versa). Extremophiles 12(1):39-50
13. Joseph GT, Huima T, Lustigman S (1998) Characterization of an Onchocerca volvulus L3-specific larval antigen, Ov-ALT-1. Mol Biochem Parasitol 96:177-183. doi:10.1016/S0166-6851(98) 00094-2
14. Kopetzki E, Schumacher G, Buckel P (1989) Control of formation of active soluble or inactive insoluble baker's yeast alphaglucosidase PI in Escherichia coli by induction and growth conditions. Mol Gen Genet 216(1):149-155
15. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157(1):105-132
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
17. Madurawe RD, Chase TE, Tsao EI, Bentley WE (2000) A recombinant lipoprotein antigen against Lyme disease expressed in E. coli: fermentor operating strategies for improved yield. Biotechnol Prog 16:571-576
18. Maizels RM, Gomez-Escobar N, Gregory WF, Murray J, Zang X (2001) Immune evasion genes from filarial nematodes. Int J Parasitol 31(9):889-898
19. Pasamontes L, Denise E, Kurt B (1986) Production of monoclonal and monospecific antibodies against non-capsid proteins of poliovirus. J Gen Virol 67:2415-2422
20. Ramachandran S, Kumar MP, Reddy MVR, Harinath BC, Nutman TB, Kaliraj P, McCarthy J (2004) The larval specific lymphatic filarial ALT-2: induction of protection using protein or DNA vaccination. Microbiol Immunol 48(12):945-955
21. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory Press, New York
22. Thirugnanam S, Pandiaraja P, Ramaswamy K, Murugan V, Gnanasekar M, Nandakumar K, Reddy MV, Kaliraj P (2007) Brugia malayi: comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model. Exp Parasitol 116(4):483-491
23. Vanam U, Pandey V, Prabhu PR, Dakshinamurthy G, Reddy MV, Kaliraj P (2009) Evaluation of immunoprophylactic efficacy of Brugia malayi transglutaminase (BmTGA) in single and multiple antigen vaccination with BmALT-2 and BmTPX for human lymphatic filariasis. Am J Trop Med Hyg 80(2):319-324
24. Waugh DS (2005) Making the most of affinity tags. Trends Biotechnol 23(6):316-320
25. World Health Organization (2006) Global programme to eliminate lymphatic filariasis: progress report on mass drug administrations in 2005. Wkly Epidemiol Rec 22:221-232
26. Yamane T, Shimizu S (1984) Fed-batch techniques in microbial processes. Adv Biochem Eng 3:147-194
27. Yano T, Kobayashi T, Shimizu S (1978) Fed-batch culture of methanol-utilizing bacterium with DO-stat. J Ferment Technol 56:416-420
28. Yee L, Blanch HW (1992) Recombinant protein expression in high cell density fed-batch cultures of E. coli. Bio/Technology 10:1550-1556
29. Yee L, Blanch HW (1993) Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli. Biotechnol Bioeng 41(8):781-790
30. Zanette D, Dundon W, Soffientini A, Sottani C, Marinelli F, Akeson A, Sarubbi E (1998) Human IL-1 receptor antagonist from Escherichia coli: large-scale microbial growth and protein purification. J Biotechnol 64:187-196