Development of a simple fed-batch process for the high-yield production of recombinant Japanese encephalitis virus protein

Applied Microbiology and Biotechnology

Volumn 86, Issue 6, 2010, Pages 1795-1803

  Tripathi, Nagesh K ^a,b   Shukla, Jyoti ^a   Biswal, Karttik C ^b   Lakshmana Rao, P V ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

^b NATIONAL INSTITUTE OF TECHNOLOGY   (India)

Author keywords

Cultivation; ELISA; Escherichia coli; Japanese encephalitis; Media optimization

Indexed keywords

CHILDREN AND ADOLESCENTS; DENATURING CONDITIONS; E. COLI; FED-BATCH CULTIVATION; FED-BATCH PROCESS; HUMAN SERUM; IN-CELL; INCLUSION BODIES; INSOLUBLE PROTEINS; MEDIA OPTIMIZATION; PURIFIED PROTEIN; RECOMBINANT E. COLI; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; VACCINE DEVELOPMENT; VIRAL PROTEINS; VIRUS INFECTION; VIRUS PROTEINS; WESTERN BLOTTING; YIELD PRODUCTION;

AFFINITY CHROMATOGRAPHY; BATCH DATA PROCESSING; CELL CULTURE; ELECTROPHORESIS; ESCHERICHIA COLI; GELS; OPTIMIZATION; PURIFICATION; SODIUM; SODIUM SULFATE; UREA; VIRUSES;

PROTEINS;

DODECYL SULFATE SODIUM; ISOPROPYL THIOGALACTOSIDE; JAPANESE ENCEPHALITIS VIRUS PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

CHROMATOGRAPHY; COLIFORM BACTERIUM; ELECTROKINESIS; GENE EXPRESSION; PROTEIN; RECOMBINATION; VACCINE; VIRAL DISEASE; VIRUS;

ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FED BATCH CULTURE; JAPANESE ENCEPHALITIS VIRUS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; QUANTUM YIELD; WESTERN BLOTTING;

ANTIBODIES, VIRAL; BIOREACTORS; BLOTTING, WESTERN; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENCEPHALITIS VIRUS, JAPANESE; ENCEPHALITIS, JAPANESE; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN M; INCLUSION BODIES; RECOMBINANT PROTEINS; VIRAL ENVELOPE PROTEINS;

ESCHERICHIA COLI; JAPANESE ENCEPHALITIS VIRUS;

EID: 77952879821     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2488-4     Document Type: Article

References (29)

1. A Abhyankar PK Dash P Saxena R Bhargava MM Parida AM Jana AK Sahni PVL Rao 2006 Comparision of a dipstick dot ELISA with commercial assays for anti-dengue virus IgM antibodies Viral Immunol 19 630 636 10.1089/vim.2006.19. 630 1:CAS:528:DC%2BD2sXhtVeqtg%3D%3D
2. BK Alka YPS Malik S Vrati 2007 Immunogenicity and protective efficacy of the E. coli expressed domain III of Japanese encephalitis virus envelope protein in mice Med Microbiol Immunol 196 227 231 10.1007/s00430-007-0043-4 1:CAS:528:DC%2BD2sXptVGms7k%3D (Pubitemid 350105922)
3. KR Babu S Swaminathan S Marten N Khanna U Rinas 2000 Production of interferon-α in high cell density cultures of recombinant Escherichia coli and its single step purification from refolded inclusion body proteins Appl Microbiol Biotechnol 53 655 660 10.1007/s002530000318 1:CAS:528: DC%2BD3cXkvVyhur0%3D
4. Bhatnagar R, Mohsin WS, Chauhan V (2008) High level constitutive production of anthrax protective antigen. US Patent 7,329,513 B2
5. K Bundo A Igarashi 1985 Antibody-capture ELISA for detection of immunoglobulin M antibodies in sera from Japanese encephalitis and dengue hemorrhagic fever patients J Virol Methods 11 15 22 10.1016/0166-0934(85)90120-X 1:STN:280:DyaL2M3jsVOmtA%3D%3D (Pubitemid 15044914)
6. D Ghosh A Basu 2009 Japanese encephalitis-a pathological and clinical perspective PLoS Negl Trop Dis 3 9 e437 10.1371/journal.pntd.0000437
7. R Kaur S Vrati 2003 Development of a recombinant vaccine against Japanese encephalitis J Neurovirol 9 421 431 1:CAS:528:DC%2BD3sXmtVeksLs%3D
8. R Khalilzadeh SA Shojaosadati N Maghsoudi J Mohammadian-Mosaabadi MR Mohammadi A Bahrami N Maleksabet MA Nassiri-Khalilli M Ebrahimi EH Naderimanesh 2004 Process development for production of recombinant human interferon-γ expressed in E. coli J Ind Microbiol Biotechnol 31 63 69 10.1007/s10295-004- 0117-x 1:CAS:528:DC%2BD2cXhvFClur8%3D (Pubitemid 38437950)
9. DJ Korz U Rinas K Hellmuth EA Sanders WD Deckwer 1995 Simple fed-batch technique for high cell density cultivation of Escherichia coli J Biotechnol 39 59 65 10.1016/0168-1656(94)00143-Z 1:CAS:528:DyaK2MXjvVemtLk%3D
10. DH Kweon NS Han KM Park JH Seo 2001 Overproduction of Phytolacca insularis protein in batch and fed-batch culture of recombinant Escherichia coli Proc Biochem 36 537 542 10.1016/S0032-9592(00)00237-5 1:CAS:528: DC%2BD3MXhtVGlsr8%3D (Pubitemid 32105229)
11. SY Lee 1996 High cell-density culture of Escherichia coli Trends Biotechnol 14 98 105 10.1016/0167-7799(96)80930-9 1:CAS:528:DyaK28XhsleqtLs%3D (Pubitemid 26079509)
12. L promoter Biotechnol Prog 14 548 553 10.1021/bp980059y 1:CAS:528:DyaK1cXkvFKgurk%3D (Pubitemid 28382646)
13. HK Lim KH Jung DH Park SI Chung 2000 Production characteristics of interferon-α using an l-arabinose promoter system in a high-cell-density culture Appl Microbiol Biotechnol 53 201 208 10.1007/s002530050009 1:CAS:528:DC%2BD3cXhsVOltrk%3D
14. CW Lin SC Wu 2003 A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with neutralizing-antibody combining sites J Virol 77 2600 2606 10.1128/JVI.77.4. 2600-2606.2003 1:CAS:528:DC%2BD3sXpvVGmuw%3D%3D (Pubitemid 36222784)
15. RD Madurawe TE Chase EI Tsao WE Bentley 2000 A recombinant lipoprotein antigen against Lyme disease expressed in E. coli: fermentor operating strategies for improved yield Biotechnol Progress 16 571 576 10.1021/bp0000555 1:CAS:528:DC%2BD3cXksVagtbo%3D (Pubitemid 30647763)
16. D Manderson R Dempster Y Chisti 2006 Recombinant vaccine against hydatidosis: production of the antigen in Escherichia coli J Ind Microb Biotechnol 33 173 182 10.1007/s10295-005-0046-3 1:CAS:528:DC%2BD28Xhs1Wru7k%3D (Pubitemid 43288306)
17. MM Parida PK Dash NK Tripathi SSR Ambuj P Saxena S Agarwal AK Sahni SP Singh AK Rathi R Bhargava A Abhyankar SK Verma PVL Rao K Sekhar 2006 Japanese encephalitis outbreak India 2005 Emerg Infect Dis 9 1427 1430
18. P Pattnaik JP Babu SK Verma V Tak PVL Rao 2007 Bacterially expressed and refolded envelope protein (D III) of dengue virus type 4 binds heparan sulphate J Chromat B 846 184 94 10.1016/j.jchromb.2006.08.051 1:CAS:528: DC%2BD2sXhtVKgsL4%3D (Pubitemid 46149513)
19. V Ravi A Desai M Balaji MP Apte L Lakshman DK Subbakrishna G Sridharan TN Dhole BV Ravikumar 2006 Development and evaluation of a rapid IgM capture ELISA (JEV-Chex) for the diagnosis of Japanese encephalitis J Clin Virol 35 429 432 10.1016/j.jcv.2005.11.004 1:CAS:528:DC%2BD28XitVGiu78%3D (Pubitemid 43324841)
20. A Shrivastva NK Tripathi M Parida PK Dash AM Jana PVL Rao 2008 Comparison of a dipstick enzyme-linked immunosorbent assay with commercial assays for detection of Japanese encephalitis virus-specific IgM antibodies J Postgrad Med 54 181 185 10.4103/0022-3859.40959 1:STN:280:DC%2BD1cvltVejtg%3D%3D (Pubitemid 352009156)
21. J Shukla R Bhargava PK Dash M Parida N Tripathi PVL Rao 2009 Cloning and expression of domain III of the envelope gene of Japanese encephalitis virus: evaluation for early clinical diagnosis by IgM ELISA J Virol Methods 158 165 170 10.1016/j.jviromet.2009.02.018 1:CAS:528:DC%2BD1MXltV2jtLk%3D
22. SM Singh AK Panda 2005 Solubilization and refolding of bacterial inclusion body proteins J Biosci Bioeng 99 303 310 10.1263/jbb.99.303 1:CAS:528:DC%2BD2MXlsFCnsrc%3D
23. T Solomon LT Thao NM Dung R Kneen NT Hung A Nisalak DW Vaughn J Farrar TT Hien NJ White MJ Cardosa 1998 Rapid diagnosis of Japanese encephalitis by using an immunoglobulin M dot enzyme immunoassay J Clin Microbiol 36 2030 2034 1:CAS:528:DyaK1cXktFWrtbY%3D
24. SV Tiroumourougane P Raghava S Srinivasan 2002 Japanese viral encephalitis Postgrad Med J 78 205 215 10.1136/pmj.78.918.205 1:STN:280:DC%2BD387pslWntw%3D%3D (Pubitemid 34293321)
25. NK Tripathi JP Babu A Shrivastva M Parida AM Jana PVL Rao 2008 Production and characterization of recombinant dengue virus type 4 envelope domain III protein J Biotechnol 134 278 286 10.1016/j.jbiotec.2008.02.001 1:CAS:528:DC%2BD1cXksValu70%3D
26. NK Tripathi A Shrivastva KC Biswal PVL Rao 2009 Optimization of culture medium for production of recombinant dengue protein in Escherichia coli Ind Biotechnol 5 179 183 10.1089/ind.2009.3.179 1:CAS:528:DC%2BD1MXht1Skt73I
27. SK Verma S Kumar N Gupta S Vedic SM Bhattacharya PVL Rao 2009 Bacterially expressed recombinant envelope protein domain III of Japanese encephalitis virus (rJEV-DIII) elicits Th1 type of immune response in BALB/c mice Vaccine 27 6905 6909 10.1016/j.vaccine.2009.09.006 1:CAS:528:DC%2BD1MXhtlCmsL%2FK
28. SC Wu CH Yu CW Lin IM Chu 2003 The domain III fragment of Japanese encephalitis virus envelpe protein: mouse immunogenicity and liposome adjuvancity Vaccine 21 2516 1522 10.1016/S0264-410X(03)00042-2 1:CAS:528:DC%2BD3sXjs1Wlsb0%3D (Pubitemid 36561344)
29. DK Yang BH Kim SI Lim JH Kwon KW Lee CU Choi CH Kweon 2006 Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine J Vet Sci 7 271 275