Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Volontè, Federica ^a   Pollegioni, Loredano ^a   Molla, Gianluca ^a   Frattini, Luca ^a   Marinelli, Flavia ^a   Piubelli, Luciano ^a  

^a UNIVERSITY OF INSUBRIA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE PROTEINS; BIOTECHNOLOGICAL APPLICATIONS; BREVIBACTERIUM STEROLICUM; CHOLESTEROL LEVELS; CHOLESTEROL OXIDASE; COFACTORS; COVALENTLY BOUND; CRUDE EXTRACT; DRUG PRODUCTION; E. COLI; EXPRESSION LEVELS; MEDIUM COMPOSITION; OVER-EXPRESSION; PROTEIN MOIETY; RECOMBINANT E. COLI; SPECIFIC ACTIVITY; TYPE II; VOLUMETRIC PRODUCTIVITY;

BIOTECHNOLOGY; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; OPTIMIZATION; PROTEINS;

CHOLESTEROL;

CHOLESTEROL OXIDASE; CHOLESTEROL OXIDASE TYPE II; FLAVINE ADENINE NUCLEOTIDE; RECOMBINANT CHOLESTEROL OXIDASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; COMPLEMENTARY DNA; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; BIOTECHNOLOGICAL PRODUCTION; BREVIBACTERIUM; BREVIBACTERIUM STEROLICUM; CELL INCLUSION; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION MEDIUM; GENE OVEREXPRESSION; NONHUMAN; PLASMID; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; AMINO ACID SEQUENCE; BIOREACTOR; BIOSYNTHESIS; CULTURE MEDIUM; ENZYMOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS;

BREVIBACTERIUM STEROLICUM; ESCHERICHIA COLI; STREPTOMYCES;

AMINO ACID SEQUENCE; BIOREACTORS; BREVIBACTERIUM; CHOLESTEROL OXIDASE; CLONING, MOLECULAR; CULTURE MEDIA; DNA, COMPLEMENTARY; ESCHERICHIA COLI; FLAVIN-ADENINE DINUCLEOTIDE; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS;

EID: 77951076300     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-33     Document Type: Article

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