Recombinant Production of Human Interleukin 6 in Escherichia coli

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Nausch, Henrik ^a   Huckauf, Jana ^a   Koslowski, Roswitha ^b   Meyer, Udo ^b   Broer, Inge ^a   Mikschofsky, Heike ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

^b Bioserv GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; CYTOPLASM PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RECOMBINANT INTERLEUKIN 6;

ARTICLE; BIOLOGICAL ACTIVITY; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOKINE PRODUCTION; ENVIRONMENTAL FACTOR; ESCHERICHIA COLI; IN VIVO STUDY; NONHUMAN; OXIDATION; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN TARGETING; SOLUBILIZATION; THERMODYNAMICS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CYTOPLASM; ESCHERICHIA COLI; GENE EXPRESSION; GENE ORDER; HUMANS; INTERLEUKIN-6; ISOPROPYL THIOGALACTOSIDE; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

ESCHERICHIA COLI;

EID: 84872824121     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0054933     Document Type: Article

References (51)

1. Riedemann NC, Guo RF, Ward PA, (2003) Novel strategies for the treatment of sepsis. Nature Medicine 9: 517-524.
2. Riedemann NC, Neff TA, Guo RF, Bernacki KD, Laudes IJ, et al. (2003) Protective effects of IL-6 blockade in sepsis are linked to reduced C5a receptor expression. Journal of Immunology 170: 503-507.
3. Wu HP, Chen CK, Chung K, Tseng JC, Hua CC, et al. (2009) Serial cytokine levels in patients with severe sepsis. Inflammation Research 58: 385-393.
4. Kim TW, Chung BH, Chang YK, (2005) Production of soluble human interleukin-6 in cytoplasm by fed-batch culture of recombinant E-coli. Biotechnology Progress 21: 524-531.
5. Spiridonova VA, Lygina AS, Anohina MM, Tupitsyn NN, (2007) Preparation of functionally active recombinant human interleukin-6. Biochemistry-Moscow 72: 424-429.
6. Baneyx F, Mujacic M, (2004) Recombinant protein folding and misfolding in Escherichia coli. Nature Biotechnology 22: 1399-1408.
7. Brakenhoff JPJ, Degroot ER, Evers RF, Pannekoek H, Aarden LA, (1987) Molecular-Cloning and Expression of Hybridoma Growth-Factor in Escherichia-Coli. Journal of Immunology 139: 4116-4121.
8. Ejima D, Watanabe M, Sato Y, Date M, Yamada N, et al. (1999) High yield refolding and purification process for recombinant human interleukin-6 expressed in Escherichia coli. Biotechnology and Bioengineering 62: 301-310.
9. Tonouchi N, Oouchi N, Kashima N, Kawai M, Nagase K, et al. (1988) High-Level Expression of Human Bsf-2/Il-6 Cdna in Escherichia-Coli Using A New Type of Expression-Preparation System. Journal of Biochemistry 104: 30-34.
10. Simpson RJ, Hammacher A, Smith DK, Matthews JM, Ward LD, (1997) Interleukin-6: Structure-function relationships. Protein Science 6: 929-955.
11. May LT, Santhanam U, Sehgal PB, (1991) On the multimeric nature of natural human interleukin-6. J Biol Chem 266: 9950-9955.
12. Villaverde A, Carrio MM, (2003) Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnology Letters 25: 1385-1395.
13. Lee EG, Baek JE, Lee SH, Kim TW, Choi JH, et al. (2009) Efficient proteolytic cleavage by insertion of oligopeptide linkers and its application to production of recombinant human interleukin-6 in Escherichia coli. Enzyme and Microbial Technology 44: 254-262.
14. Li YY, Chen CX, von Specht BU, Hahn HP, (2002) Cloning and hemolysin-mediated secretory expression of a codon-optimized synthetic human interleukin-6 gene in Escherichia coli. Protein Expression and Purification 25: 437-447.
15. Huang CJ, Lin H, Yang XM, (2012) Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements. Journal of Industrial Microbiology & Biotechnology 39: 383-399.
16. de Marco A (2009) Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli. Microbial Cell Factories 8.
17. Salinas G, Pellizza L, Margenat M, Flo M, Fernandez C, (2011) Tuned Escherichia coli as a host for the expression of disulfide-rich proteins. Biotechnology Journal 6: 686-699.
18. Terpe K, (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology 60: 523-533.
19. Cong YH, Pawlisz E, Bryant P, Balan S, Laurine E, et al. (2012) Site-Specific PEGylation at Histidine Tags. Bioconjugate Chemistry 23: 248-263.
20. de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B (2007) Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E-coli. Bmc Biotechnology 7.
21. Schlapschy M, Grimm S, Skerra A, (2006) A system for concomitant overexpression of four periplasmic folding catalysts to improve secretory protein production in Escherichia coli. Protein Engineering Design & Selection 19: 385-390.
22. Vazquez E, Corchero JL, Villaverde A (2011) Post-production protein stability: trouble beyond the cell factory. Microbial Cell Factories 10.
23. Ferrer-Miralles N, Domingo-Espin J, Corchero JL, Vazquez E, Villaverde A (2009) Microbial factories for recombinant pharmaceuticals. Microbial Cell Factories 8.
24. Bessette PH, Aslund F, Beckwith J, Georgiou G, (1999) Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proceedings of the National Academy of Sciences of the United States of America 96: 13703-13708.
25. Lauber T, Marx UC, Schulz A, Kreutzmann P, Rosch P, et al. (2001) Accurate disulfide formation in Escherichia coli: Overexpression and characterization of the first domain (HF6478) of the multiple Kazal-type inhibitor LEKTI. Protein Expression and Purification 22: 108-112.
26. Lobel L, Pollak S, Lustbader B, Klein J, Lustbader JW, (2002) Bacterial expression of a natively folded extracellular domain fusion protein of the hFSH receptor in the cytoplasm of Escherichia coli. Protein Expression and Purification 25: 124-133.
27. Venturi M, Seifert C, Hunte C, (2002) High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm. Journal of Molecular Biology 315: 1-8.
28. Waegeman H, Soetaert W, (2011) Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering. Journal of Industrial Microbiology & Biotechnology 38: 1891-1910.
29. Negrete A, Ng WI, Shiloach J, (2010) Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21). Microb Cell Fact 9: 75.
30. Phue JN, Noronha SB, Hattacharyya R, Wolfe AJ, Shiloach J, (2005) Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analyses. Biotechnol Bioeng 90: 805-820.
31. Phue JN, Shiloach J, (2004) Transcription levels of key metabolic genes are the cause for different glucose utilization pathways in E. coli B (BL21) and E. coli K (JM109). J Biotechnol 109: 21-30.
32. Shiloach J, Kaufman J, Guillard AS, Fass R, (1996) Effect of glucose supply strategy on acetate accumulation, growth, and recombinant protein production by Escherichia coli BL21 (lambdaDE3) and Escherichia coli JM109. Biotechnol Bioeng 49: 421-428.
33. Waegeman H, Maertens J, Beauprez J, De Mey M, Soetaert W, (2012) Effect of iclR and arcA deletions on physiology and metabolic fluxes in Escherichia coli BL21 (DE3). Biotechnol Lett 34: 329-337.
34. Gottesman S, (1989) Genetics of proteolysis in Escherichia coli*. Annu Rev Genet 23: 163-198.
35. Gottesman S, (1996) Proteases and their targets in Escherichia coli. Annu Rev Genet 30: 465-506.
36. Seo JH, Kang DG, Cha HJ, (2003) Comparison of cellular stress levels and green-fluorescent-protein expression in several Escherichia coli strains. Biotechnology and Applied Biochemistry 37: 103-107.
37. Mergulhao FJM, Summers DK, Monteiro GA, (2005) Recombinant protein secretion in Escherichia coli. Biotechnology Advances 23: 177-202.
38. Ni Y, Chen R, (2009) Extracellular recombinant protein production from Escherichia coli. Biotechnology Letters 31: 1661-1670.
39. Jeong KJ, Lee SY, (2001) Secretory production of human granulocyte colony-stimulating factor in Escherichia coli. Protein Expression and Purification 23: 311-318.
40. Xu YL, Yasin A, Wucherpfennig T, Chou CP, (2008) Enhancing functional expression of heterologous lipase in the periplasm of Escherichia coli. World Journal of Microbiology & Biotechnology 24: 2827-2835.
41. Rose-John S, Scheller J, Elson G, Jones SA, (2006) Interleukin-6 biology is coordinated by membrane-bound and soluble receptors: role in inflammation and cancer. Journal of Leukocyte Biology 80: 227-236.
42. Sorensen HP, Mortensen KK, (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. Journal of Biotechnology 115: 113-128.
43. Hoffmann F, van den Heuvel J, Zidek N, Rinas U, (2004) Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale. Enzyme and Microbial Technology 34: 235-241.
44. Kiefhaber T, Rudolph R, Kohler HH, Buchner J, (1991) Protein Aggregation Invitro and Invivo- A Quantitative Model of the Kinetic Competition Between Folding and Aggregation. Bio-Technology 9: 825-829.
45. De Marco V, Stier G, Blandin S, de Marco A, (2004) The solubility and stability of recombinant proteins are increased by their fusion to NusA. Biochemical and Biophysical Research Communications 322: 766-771.
46. Fox JD, Routzahn KM, Bucher MH, Waugh DS, (2003) Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. Febs Letters 537: 53-57.
47. Marblestone JG, Edavettal SC, Lim Y, Lim P, Zuo X, et al. (2006) Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO. Protein Science 15: 182-189.
48. Braun P, Hu YH, Shen BH, Halleck A, Koundinya M, et al. (2002) Proteome-scale purification of human proteins from bacteria. Proceedings of the National Academy of Sciences of the United States of America 99: 2654-2659.
49. Hammarstrom M, Hellgren N, Van den Berg S, Berglund H, Hard T, (2002) Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli. Protein Science 11: 313-321.
50. Niiranen L, Espelid S, Karlsen CR, Mustonen M, Paulsen SM, et al. (2007) Comparative expression study to increase the solubility of cold adapted Vibrio proteins in Escherichia coli. Protein Expression and Purification 52: 210-218.
51. Shih YP, Kung WM, Chen JC, Yeh CH, Wang AHJ, et al. (2002) High-throughput screening of soluble recombinant proteins. Protein Science 11: 1714-1719.