MemStar: A one-shot Escherichia coli-based approach for high-level bacterial membrane protein production

FEBS Letters

Volumn 588, Issue 20, 2014, Pages 3761-3769

  Lee, Chiara ^a   Kang, Hae Joo ^a   Hjelm, Anna ^b   Qureshi, Abdul Aziz ^b   Nji, Emmanuel ^b   Choudhury, Hassanul ^a,c,d   Beis, Konstantinos ^a,c,d   De Gier, Jan Willem ^b   Drew, David ^a,b  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b STOCKHOLM UNIVERSITY   (Sweden)

^c DIAMOND LIGHT SOURCE LTD   (United Kingdom)

^d RUTHERFORD APPLETON LABORATORY   (United Kingdom)

Author keywords

Escherichia coli; High throughput; Membrane protein production; X ray crystallography

Indexed keywords

BACTERIAL PROTEIN; ISOPROPYL THIOGALACTOSIDE; MEMBRANE PROTEIN; PROTEIN NAPA; SODIUM PROTON EXCHANGE PROTEIN; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN; OUTER MEMBRANE PROTEIN;

ARTICLE; BACTERIAL STRAIN; BIOTECHNOLOGICAL PROCEDURES; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FLOW CYTOMETRY; MEMBRANE PROTEIN SINGLE SHOT AMPLIFICATION RECIPE; NONHUMAN; OPTICAL DENSITY; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; CELL FRACTIONATION; CHEMISTRY; FRACTIONATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HIGH-THROUGHPUT; MEMBRANE PROTEIN PRODUCTION; METABOLISM; METHODOLOGY; MICROBIOLOGY; X-RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI; HIGH-THROUGHPUT; MEMBRANE PROTEIN PRODUCTION; X-RAY CRYSTALLOGRAPHY;

BACTERIAL OUTER MEMBRANE PROTEINS; CELL FRACTIONATION; CHEMICAL FRACTIONATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; INDUSTRIAL MICROBIOLOGY;

EID: 84907286117     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2014.08.025     Document Type: Article

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