Reliable scale-up of membrane protein over-expression by bacterial auto-induction: From microwell plates to pilot scale fermentations

Molecular Membrane Biology

Volumn 25, Issue 8, 2008, Pages 588-598

  Deacon, Sarah E ^a   Roach, Peter C J ^a   Postis, Vincent L G ^a   Wright, Gareth S A ^a   Xia, Xiaobing ^a,b   Phillips, Simon E V ^a,c   Paul Knox, J ^a   Henderson, Peter J F ^a   Mcpherson, Michael J ^a   Baldwin, Stephen A ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c DIAMOND LIGHT SOURCE LTD   (United Kingdom)

Author keywords

Auto induction; Fermentation; Membrane protein; Protein expression; Scale up

Indexed keywords

GLUCOSE; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; LACTOSE; MEMBRANE PROTEIN; SUCCINIC ACID;

ARTICLE; BATCH PROCESS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTALLIZATION; FERMENTATION; MEDICAL INFORMATION SYSTEM; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN STRUCTURE; SCALE UP; STRUCTURE ANALYSIS;

BIOREACTORS; CRYSTALLOGRAPHY, X-RAY; CULTURE MEDIA; FERMENTATION; GLUCOSE; GLYCEROL; HYDROGEN-ION CONCENTRATION; ISOPROPYL THIOGALACTOSIDE; LACTOSE; MEMBRANE PROTEINS; OXYGEN; PILOT PROJECTS; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION;

BACTERIA (MICROORGANISMS);

EID: 57049129965     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.1080/09687680802511774     Document Type: Article

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