Inhibition of autophagy enhances the radiosensitivity of nasopharyngeal carcinoma by reducing Rad51 expression

Oncology Reports

Volumn 32, Issue 5, 2014, Pages 1905-1912

  Mo, Ning ^a   Lu, Yong Kui ^a   Xie, Wei Min ^a   Liu, Yan ^a   Zhou, Wen Xian ^a   Wang, Hong Xue ^a   Nong, Li ^a   Jia, Yu Xian ^a   Tan, Ai Hua ^a   Chen, Ying ^a   Li, Shan Shan ^a   Luo, Bao Hua ^a  

^a GUANGXI MEDICAL UNIVERSITY   (China)

Author keywords

Autophagy; Autophagy related 5; Nasopharyngeal carcinoma; Rad51; Radiosensitivity

Indexed keywords

AUTOPHAGY PROTEIN 5; CELL DNA; DOUBLE STRANDED DNA; MESSENGER RNA; PROTEIN INHIBITOR; RAD51 PROTEIN; ATG5 PROTEIN, HUMAN; DNA; MICROTUBULE ASSOCIATED PROTEIN; RAD51 PROTEIN, HUMAN; SMALL INTERFERING RNA;

APOPTOSIS; ARTICLE; AUTOPHAGY; CARCINOMA CELL; CELL CYCLE PROGRESSION; CELL CYCLE REGULATION; CONTROLLED STUDY; DOUBLE STRANDED DNA BREAK; GENE EXPRESSION REGULATION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; IONIZING RADIATION; NASOPHARYNX CARCINOMA; PROTEIN EXPRESSION; PROTEIN TARGETING; RADIOSENSITIVITY; REGULATORY MECHANISM; SENSITIVITY ANALYSIS; DNA DAMAGE; DRUG EFFECTS; GENETICS; METABOLISM; NASOPHARYNGEAL NEOPLASMS; RADIATION DOSE; RADIATION RESPONSE; TUMOR CELL LINE;

AUTOPHAGY; CELL LINE, TUMOR; DNA; DNA DAMAGE; HUMANS; MICROTUBULE-ASSOCIATED PROTEINS; NASOPHARYNGEAL NEOPLASMS; RAD51 RECOMBINASE; RADIATION TOLERANCE; RNA, SMALL INTERFERING;

EID: 84907285869     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2014.3427     Document Type: Article

References (35)

1. Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev 15: 1765-1777, 2006.
2. Hou H, Li D, Cheng D, Li L, Liu Y and Zhou Y: Cellular redox status regulates emodin-induced radiosensitization of nasopharyngeal carcinoma cells in vitro and in vivo. J Pharmaceutics 2013: 2013.
3. Verheij M and Bartelink H: Radiation-induced apoptosis. Cell Tissue Res 301: 133-142, 2000.
4. Zois CE and Koukourakis MI: Radiation-induced autophagy in normal and cancer cells: towards novel cytoprotection and radio.sensitization policies? Autophagy 5: 442-450, 2009.
5. Liu WM, Huang P, Kar N, et al: Lyn facilitates glioblastoma cell survival under conditions of nutrient deprivation by promoting autophagy. PloS One 8: e70804, 2013.
6. Oehme I, Linke JP, Böck BC, et al: Histone deacetylase 10 promotes autophagy-mediated cell survival. Proc Natl Acad Sci USA 110: E2592-E2601, 2013.
7. Selvakumaran M, Amaravadi RK, Vasilevskaya IA and O'Dwyer PJ: Autophagy inhibition sensitizes colon cancer cells to antiangiogenic and cytotoxic therapy. Clin Cancer Res 19: 2995-3007, 2013.
8. Lomonaco SL, Finniss S, Xiang C, et al: The induction of autophagy by γ-radiation contributes to the radioresistance of glioma stem cells. Int J Cancer 125: 717-722, 2009.
9. Apel A, Herr I, Schwarz H, Rodemann HP and Mayer A: Blocked autophagy sensitizes resistant carcinoma cells to radiation therapy. Cancer Res 68: 1485-1494, 2008.
10. Wang Y, Yin W and Zhu X: Blocked autophagy enhances radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 in vitro. Acta Otolaryngol 134: 105-110, 2014.
11. Mazin AV and Mazina OM: RAD51 is a key protein of DNA repair and homologous recombination in humans. 281-302, 2013.
12. Vispé S, Cazaux C, Lesca C and Defais M: Overexpression of Rad51 protein stimulates homologous recombination and increases resistance of mammalian cells to ionizing radiation. Nucleic Acids Res 26: 2859-2864, 1998.
13. Somaiah N, Yarnold J, Lagerqvist A, Rothkamm K and Helleday T: Homologous recombination mediates cellular resistance and fraction size sensitivity to radiation therapy. Radiother Oncol 108: 155-161, 2013.
14. Zhou ZR, Zhu XD, Zhao W, et al: Poly(ADP-ribose) polymerase-1 regulates the mechanism of irradiation-induced CNE-2 human nasopharyngeal carcinoma cell autophagy and inhibition of autophagy contributes to the radiation sensitization of CNE-2 cells. Oncol Rep 29: 2498-2506, 2013.
15. Takasu H, Sugita A, Uchiyama Y, et al: c-Fos protein as a target of anti-osteoclastogenic action of vitamin D, and synthesis of new analogs. J Clin Invest 116: 528-535, 2006.
16. Menna-Barreto RF, Salomão K, Dantas AP, et al: Different cell death pathways induced by drugs in Trypanosoma cruzi: an ultrastructural study. Micron 40: 157-168, 2009.
17. Dickey JS, Redon CE, Nakamura AJ, Baird BJ, Sedelnikova OA and Bonner WM: H2AX: functional roles and potential applications. Chromosoma 118: 683-692, 2009.
18. Bae Y, Jung SH, Kim GY, Rhim H and Kang S: Hip2 ubiquitinconjugating enzyme overcomes radiation-induced G2/M arrest. Biochim Biophys Acta 1833: 2911-2921, 2013.
19. Miyata H, Doki Y, Yamamoto H, et al: Overexpression of CDC25B overrides radiation-induced G2-M arrest and results in increased apoptosis in esophageal cancer cells. Cancer Res 61: 3188-3193, 2001.
20. Rodemann HP: Molecular radiation biology: perspectives for radiation oncology. Radiother Oncol 92: 293-298, 2009.
21. Huang F, Motlekar NA, Burgwin CM, Napper AD, Diamond SL and Mazin AV: Identification of specific inhibitors of human RAD51 recombinase using high-throughput screening. ACS Chem Biol 6: 628-635, 2011.
22. Budke B, Logan HL, Kalin JH, et al: RI-1: a chemical inhibitor of RAD51 that disrupts homologous recombination in human cells. Nucleic Acids Res 40: 7347-7357, 2012.
23. Eisenberg-Lerner A and Kimchi A: The paradox of autophagy and its implication in cancer etiology and therapy. Apoptosis 14: 376-391, 2009.
24. Tsuchihara K, Fujii S and Esumi H: Autophagy and cancer: dynamism of the metabolism of tumor cells and tissues. Cancer Lett 278: 130-138, 2009.
25. White E: Deconvoluting the context-dependent role for autophagy in cancer. Nat Rev Cancer 12: 401-410, 2012.
26. -/low breast cancer stem/progenitor cells to tamoxifen. and doxorubicin.induced autophagy. Int J Mol Med 31: 1477-1483, 2013.
27. Tekirdag KA, Korkmaz G, Ozturk DG, Agami R and Gozuacik D: MIR181A regulates starvation-And rapamycin.induced autophagy through targeting of ATG5. Autophagy 9: 374-385, 2013.
28. Zhou Y, Wang HD, Zhu L, et al: Knockdown of Nrf2 enhances autophagy induced by temozolomide in U251 human glioma cell line. Oncol Rep 29: 394-400, 2013.
29. Liang N, Jia L, Liu Y, et al: ATM pathway is essential for ionizing radiation-induced autophagy. Cell Signal 25: 2530-2539, 2013.
30. Chaachouay H, Ohneseit P, Toulany M, Kehlbach R, Multhoff G and Rodemann HP: Autophagy contributes to resistance of tumor cells to ionizing radiation. Radiother Oncol 99: 287-292, 2011.
31. Pawlik TM and Keyomarsi K: Role of cell cycle in mediating sensitivity to radiotherapy. Int J Radiat Oncol Biol Phys 59: 928-942, 2004.
32. Corcelle E, Nebout M, Bekri S, et al: Disruption of autophagy at the maturation step by the carcinogen lindane is associated with the sustained mitogen-Activated protein kinase/extracellular signal-regulated kinase activity. Cancer Res 66: 6861-6870, 2006.
33. Takenaka T, Yoshino I, Kouso H, et al: Combined evaluation of Rad51 and ERCC1 expressions for sensitivity to platinum agents in non-small cell lung cancer. Int J Cancer 121: 895-900, 2007.
34. Du LQ, Wang Y, Wang H, Cao J, Liu Q and Fan F-Y: Knockdown of Rad51 expression induces radiation-And chemo-sensitivity in osteosarcoma cells. Med Oncol 28: 1481-1487, 2011.
35. Yamamori T, Meike S, Nagane M, Yasui H and Inanami O: ER stress suppresses DNA double-strand break repair and sensitizes tumor cells to ionizing radiation by stimulating proteasomal degradation of Rad51. FEBS Lett 587: 3348-3353, 2013.