The necessity of and strategies for improving confidence in the accuracy of western blots

Expert Review of Proteomics

Volumn 11, Issue 5, 2014, Pages 549-560

  Ghosh, Rajeshwary ^a   Gilda, Jennifer E ^a   Gomes, Aldrin V ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

housekeeping protein; immunoblot; loading control; quantification; stain free gel; total protein normalization; western blotting; western blotting accuracy; western blotting strategy

Indexed keywords

BETA ACTIN; BETA TUBULIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; POLYACRYLAMIDE GEL; PROTEIN ANTIBODY; ANTIBODY; PROTEIN;

ACCURACY; ANTIBODY BLOOD LEVEL; ANTIBODY SPECIFICITY; AUTOMATION; CHEMOLUMINESCENCE; FLUORESCENCE; HUMAN; INCUBATION TIME; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; RELIABILITY; REVIEW; WESTERN BLOTTING; CHEMISTRY; PROCEDURES; STAINING; STANDARD; STANDARDS;

ANTIBODIES; BLOTTING, WESTERN; PROTEINS; REFERENCE STANDARDS; STAINING AND LABELING;

EID: 84907276168     PISSN: 14789450     EISSN: 17448387     Source Type: Journal    
DOI: 10.1586/14789450.2014.939635     Document Type: Review

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