Qualitative and quantitative evaluation of Simon™, A new CE-based automated Western blot system as applied to vaccine development

Electrophoresis

Volumn 33, Issue 17, 2012, Pages 2790-2797

  Rustandi, Richard R ^a   Loughney, John W ^a   Hamm, Melissa ^a   Hamm, Christopher ^a   Lancaster, Catherine ^a   Mach, Anna ^a   Ha, Sha ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Automated Western; Capillary electrophoresis; Simon ; Tecan; Vaccine

Indexed keywords

ANTIBODIES; AUTOMATION; CAPILLARY ELECTROPHORESIS; CROSSLINKING; VIRUSES;

AUTOMATED WESTERN; CAPILLARY ISOELECTRIC FOCUSING; PROTEIN SEPARATIONS; QUALITATIVE EVALUATIONS; QUANTITATIVE EVALUATION; SDS-PAGE; SIMON™; TECAN; VACCINE DEVELOPMENT; WESTERN BLOTS;

VACCINES;

ANTIBODY; HORSERADISH PEROXIDASE; VACCINE;

ACCURACY; ANALYTICAL EQUIPMENT; ARTICLE; AUTOMATION; CAPILLARY ELECTROPHORESIS; CHEMOLUMINESCENCE; CONTROLLED STUDY; DRUG SCREENING; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; WESTERN BLOTTING;

EID: 84866039188     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201200095     Document Type: Article

References (23)

1. Lacher, N. A., Roberts, R. K., He, Y., Cargill, H., Kearns, K. M., Holovics, H., Ruesch, M. N., J. Sep. Sci. 2010, 33, 218-227.
2. Rustandi, R. R., Washabaugh, M. W., Wang, Y., Electrophoresis 1998, 29, 3612-3620.
3. Rustandi, R. R., Wang, Y., Electrophoresis 2011, 32, 3078-3084.
4. Sosic, Z., Houde, D., Blum, A., Carlage, T., Lyubarskaya, Y., Electrophoresis 2008, 29, 4368-4376.
5. He, X. Z., Que, A. H., Mo, J. J., Electrophoresis 2009, 30, 714-722.
6. Anderson, C. L., Wang, Y., Rustandi, R. R., Electrophoresis 2012, 33, 1538-1544.
7. Towbin, H., Staehelin, T., Gordon, J., Proc. Natl. Acad. Sci. USA 1979, 76, 4350-4354.
8. Renart, J., Reiser, J., Stark, G. R., Proc. Natl. Acad. Sci. USA 1979, 76, 3116-3120.
9. Kurien, B. T., Scofield, R. H., Methods 2006, 38, 283-293.
10. Aksamitiene, E., Hoek, J. B., Kholodenko, B., Kiyatkin, A., Electrophoresis 2007, 28, 3163-3173.
11. Kuczius, T., Brandstädter, L., Karch, H., Langeveld, J. P. M., Anal. Biochem. 2011, 409, 260-266.
12. Invitrogen, iBlot® Dry Blotting System for Western; http://www.invitrogen.com/etc/medialib/en/filelibrary/protein_expression/pdfs.Par.7002.File.dat/iblotsystem_man.pdf.
13. Zellner, M., Babeluk, R., Diestinger, M., Pirchegger, P., Skeledzic, S., Oehler, R., Electrophoresis 2008, 29, 3621-3627.
14. Ornberg, R. L., Harper, T. F., Liu, H., Nat. Methods 2005, 2, 79-81.
15. Gassmann, M., Grenacher, B., Rohde, B., Vogel, J., Electrophoresis 2009, 30, 1845-1855.
16. Koller, A., Wätzig, H., Electrophoresis 2005, 26, 2470-2475.
17. O'Neill, R. A., Bhamidipati, A., Bi, X., Deb-Basu, D., Cahill, L., Ferrante, J., Gentalen, E., Glazer, M., Gossett, J., Hacker, K., Kirby, C., Knittle, J., Loder, R., Mastroieni, C., MacLaren, M., Mills, T., Nguyen, U., Parker, N., Rice, A., Roach, D., Suich, D., Voehringer, D., Voss, K., Yang, J., Yang, T., Vander Horn, P. B., Proc. Natl. Acad. Sci. USA 2006, 103, 16153-16158.
18. Fan, A. C., Deb-Basu, D., Orban, M. W., Gotlib, J. R., Natkunam, Y., O'Neill, R., Padua, R. -A., Xu, L., Taketa, D., Shirer, A. E., Beer, S., Yee, A. X., Voehringer, D. W., Felsher, D. W, Nat. Med. 2009, 15, 566-571.
19. Wenz, C., Rüfer, A., Electrophoresis 2009, 30, 4264-4269.
20. Nguyen, U., Squaglia, N., Boge, A., Fung, P. A., Nat. Methods 2011, 8, v-vi.
21. Huang, Y. -C., Tanimoto, K., Tanne, Y., Kamiya, T., Kunimatsu, R., Michida, M., Yoshioka, M., Yoshimi, Y., Kato, Y., Tanne, K., Cell Tissue Res. 2010, 342, 205-212.
22. Satheshkumar, P. S., Moss, B., J. Virol. 2009, 83, 12822-12832.
23. Shihabi, Z. K., J. Chromatogr. A 2000, 902, 107-117.