메뉴 건너뛰기




Volumn 869, Issue , 2012, Pages 451-464

Protein stains and applications

Author keywords

2 DE; Coomassie Brilliant Blue; Fluorescent stains; Pre electrophoresis staining; SDS PAGE; Silver nitrate; Zinc staining

Indexed keywords

FLUORESCENT DYE; PROTEIN; BENZOPYRAN DERIVATIVE; COLORING AGENT; COOMASSIE BRILLIANT BLUE; EPICOCCONONE; FUCHSINE; FURAN DERIVATIVE; KETONE; NILE RED; ORGANOMETALLIC COMPOUND; OXAZINE DERIVATIVE; SILVER NITRATE; ZINC SULFATE;

EID: 84865807632     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-821-4_39     Document Type: Article
Times cited : (6)

References (41)
  • 1
    • 71549136814 scopus 로고    scopus 로고
    • Protein gel staining methods: An introduction and overview
    • Steinberg TH (2009) Protein gel staining methods: an introduction and overview. Methods Enzymol 463:541-63
    • (2009) Methods Enzymol , vol.463 , pp. 541-563
    • Steinberg, T.H.1
  • 2
    • 0025234334 scopus 로고
    • Protein analysis using high resolution twodimensional polyacrylamide gel electrophoresis
    • In: Deutscher MP (ed). Academic, San Diego, CA
    • Dunbar BS, Kimura H, Timmons TM (1990) Protein analysis using high resolution twodimensional polyacrylamide gel electrophoresis. In: Deutscher MP (ed) Methods in enzymology, vol 182. Academic, San Diego, CA, pp 441-459
    • (1990) Methods in Enzymology , vol.182 , pp. 441-459
    • Dunbar, B.S.1    Kimura, H.2    Timmons, T.M.3
  • 3
    • 0025255109 scopus 로고
    • One-dimensional gel electrophoresis
    • In: Deutscher MP (ed). Academic, San Diego, CA
    • Garfin DE (1990) One-dimensional gel electrophoresis. In: Deutscher MP (ed) Methods in enzymology, vol 182. Academic, San Diego, CA, pp 425-441
    • (1990) Methods in Enzymology , vol.182 , pp. 425-441
    • Garfin, D.E.1
  • 4
    • 0025232283 scopus 로고
    • Isoelectric Focusing
    • In: Deutscher MP (ed). Academic, San Diego, CA
    • Garfin DE (1990) Isoelectric focusing. In: Deutscher MP (ed) Methods in enzymology, vol 182. Academic, San Diego, CA, pp 459-478
    • (1990) Methods in Enzymology , vol.182 , pp. 459-478
    • Garfin, D.E.1
  • 5
    • 0025218376 scopus 로고
    • Gel-staining techniques
    • In: Deutscher MP (ed). Academic, San Diego, CA, xxix+894pp
    • Merril CR (1990) Gel-staining techniques. In: Deutscher MP (ed) Methods in enzymology, vol 182. Academic, San Diego, CA, pp 477-488, xxix+894pp
    • (1990) Methods in Enzymology , vol.182 , pp. 477-488
    • Merril, C.R.1
  • 6
    • 33750575732 scopus 로고    scopus 로고
    • Protein stains for proteomic applications: Which, when, why
    • Miller I, Crawford J, Gianazza E (2006) Protein stains for proteomic applications: which, when, why? Proteomics 6:5385-5408
    • (2006) Proteomics , vol.6 , pp. 5385-5408
    • Miller, I.1    Crawford, J.2    Gianazza, E.3
  • 7
    • 0034111635 scopus 로고    scopus 로고
    • A thousand points of light: The application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics
    • Patton WF (2000) A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics. Electrophoresis 21:1123-1144
    • (2000) Electrophoresis , vol.21 , pp. 1123-1144
    • Patton, W.F.1
  • 8
    • 0037023859 scopus 로고    scopus 로고
    • Detection technologies in proteome analysis
    • Patton WF (2002) Detection technologies in proteome analysis. J Chromatogr B 771:3-31
    • (2002) J Chromatogr B , vol.771 , pp. 3-31
    • Patton, W.F.1
  • 9
    • 33644670965 scopus 로고    scopus 로고
    • The Coomassie chronicles: Past, present and future perspectives in polyacrylamide gel staining
    • Smejkal GB (2004) The Coomassie chronicles: past, present and future perspectives in polyacrylamide gel staining. Expert Rev Proteomics 1:381-387
    • (2004) Expert Rev Proteomics , vol.1 , pp. 381-387
    • Smejkal, G.B.1
  • 10
    • 0023931883 scopus 로고
    • Improved staining in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250
    • Neuhoff V, Arold N, Taube D, Ehrhardt W (1988) Improved staining in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255-262
    • (1988) Electrophoresis , vol.9 , pp. 255-262
    • Neuhoff, V.1    Arold, N.2    Taube, D.3    Ehrhardt, W.4
  • 11
    • 3042665732 scopus 로고    scopus 로고
    • Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis
    • Candiano G, Bruschi M, Musante L et al (2004) Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25:1327-1333
    • (2004) Electrophoresis , vol.25 , pp. 1327-1333
    • Candiano, G.1    Bruschi, M.2    Musante, L.3
  • 12
    • 0025053763 scopus 로고
    • Mechanisms of protein silver staining in polyacrylamide gels: A 10-year synthesis
    • Rabilloud T (1990) Mechanisms of protein silver staining in polyacrylamide gels: a 10-year synthesis. Electrophoresis 11:785-794
    • (1990) Electrophoresis , vol.11 , pp. 785-794
    • Rabilloud, T.1
  • 13
    • 71549151005 scopus 로고    scopus 로고
    • Silver staining of 2-D gels
    • In: Walker JM (ed). Humana Press, Totowa, NJ
    • Poland J, Rabilloud T, Sinha P (2005) Silver staining of 2-D gels. In: Walker JM (ed) The proteomics protocol handbook. Humana Press, Totowa, NJ, pp 215-222
    • (2005) The Proteomics Protocol Handbook , pp. 215-222
    • Poland, J.1    Rabilloud, T.2    Sinha, P.3
  • 14
    • 71549116502 scopus 로고    scopus 로고
    • Zn2+ reverse staining technique
    • In: Walker JM (ed). Humana Press, Totowa, NJ
    • Fernandez-Patron C (2005) Zn2+ reverse staining technique. In: Walker JM (ed) The proteomics protocol handbook. Humana Press, Totowa, NJ, pp 215-222
    • (2005) The Proteomics Protocol Handbook , pp. 215-222
    • Fernandez-Patron, C.1
  • 15
    • 0031785395 scopus 로고    scopus 로고
    • Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: Generalization of the reverse-staining technique
    • Fernandez-Patron C, Castellanos-Serra L, Hardy E et al (1998) Understanding the mechanism of the zinc-ion stains of biomacromolecules in electrophoresis gels: generalization of the reverse-staining technique. Electrophoresis 19:2398-2406
    • (1998) Electrophoresis , vol.19 , pp. 2398-2406
    • Fernandez-Patron, C.1    Castellanos-Serra, L.2    Hardy, E.3
  • 16
    • 33750576480 scopus 로고    scopus 로고
    • Rapid, sensitive detection of proteins in minigels with fluorescent dyes
    • Coomassie Fluor Orange, SYPRO Orange, SYPRO Red, SYPRO Tangerine, Protein Gel Stains. In: Walker JM (ed). Humana Press, Totowa, NJ
    • Steinberg TH, Hart CR, Patton WF (2005) Rapid, Sensitive Detection of Proteins in Minigels with Fluorescent Dyes. Coomassie Fluor Orange, SYPRO Orange, SYPRO Red, SYPRO Tangerine, Protein Gel Stains. In: Walker JM (ed), The proteomics handbook. Humana Press, Totowa, NJ, pp 215-222
    • (2005) The Proteomics Handbook , pp. 215-222
    • Steinberg, T.H.1    Hart, C.R.2    Patton, W.F.3
  • 17
    • 0035050882 scopus 로고    scopus 로고
    • Fluorescent labeling of proteins with Nile red and 2-methoxy-2, 4-diphenyl-3(2H)-furanone: Physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots
    • Daban JR (2001) Fluorescent labeling of proteins with Nile red and 2-methoxy-2, 4-diphenyl-3(2H)-furanone: physicochemical basis and application to the rapid staining of sodium dodecyl sulfate polyacrylamide gels and Western blots. Electrophoresis 22:874-880
    • (2001) Electrophoresis , vol.22 , pp. 874-880
    • Daban, J.R.1
  • 18
    • 0026409394 scopus 로고
    • Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels
    • Daban JR, Bartolome S, Samso M (1991) Use of the hydrophobic probe Nile red for the fluorescent staining of protein bands in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 199:169-174
    • (1991) Anal Biochem , vol.199 , pp. 169-174
    • Daban, J.R.1    Bartolome, S.2    Samso, M.3
  • 19
    • 0043210665 scopus 로고    scopus 로고
    • Epicocconone, a novel fluorescent compound from the fungus epicoccumnigrum
    • Bell PJ, Karuso P (2003) Epicocconone, a novel fluorescent compound from the fungus epicoccumnigrum. J Am Chem Soc 125: 9304-9305
    • (2003) J Am Chem Soc , vol.125 , pp. 9304-9305
    • Bell, P.J.1    Karuso, P.2
  • 20
    • 4444305698 scopus 로고    scopus 로고
    • Characterization of dynamic and steadystate protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry
    • Schulenberg B, Goodman TN, Aggeler R et al (2004) Characterization of dynamic and steadystate protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry. Electrophoresis 15:2526-2532
    • (2004) Electrophoresis , vol.15 , pp. 2526-2532
    • Schulenberg, B.1    Goodman, T.N.2    Aggeler, R.3
  • 21
    • 4444286595 scopus 로고    scopus 로고
    • Focused proteomics: Monoclonal antibodybased isolation of the oxidative phosphorylation machinery and detection of phosphoproteins using a fluorescent phosphoprotein gel stain
    • Murray J, Marusich MF, Capaldi RA et al (2004) Focused proteomics: monoclonal antibodybased isolation of the oxidative phosphorylation machinery and detection of phosphoproteins using a fluorescent phosphoprotein gel stain. Electrophoresis 15:2520-2525
    • (2004) Electrophoresis , vol.15 , pp. 2520-2525
    • Murray, J.1    Marusich, M.F.2    Capaldi, R.A.3
  • 22
    • 0038034950 scopus 로고    scopus 로고
    • Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye
    • Schulenberg B, Aggeler R, Beechem JM et al (2003) Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye. J Biol Chem 278:27251-5
    • (2003) J Biol Chem , vol.278 , pp. 27251-27255
    • Schulenberg, B.1    Aggeler, R.2    Beechem, J.M.3
  • 23
    • 12444315072 scopus 로고    scopus 로고
    • Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology
    • Steinberg TH, Agnew BJ, Gee KR et al (2003) Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology. Proteomics 3:1128-44
    • (2003) Proteomics , vol.3 , pp. 1128-1144
    • Steinberg, T.H.1    Agnew, B.J.2    Gee, K.R.3
  • 24
    • 4444254693 scopus 로고    scopus 로고
    • Combining microscale solution-phase isoelectric focusing with Multiplexed Proteomics dye staining to analyze protein post-translational modifications
    • Schulenberg B, Patton WF (2004) Combining microscale solution-phase isoelectric focusing with Multiplexed Proteomics dye staining to analyze protein post-translational modifications. Electrophoresis 25:2539-44
    • (2004) Electrophoresis , vol.25 , pp. 2539-2544
    • Schulenberg, B.1    Patton, W.F.2
  • 25
    • 4444236379 scopus 로고    scopus 로고
    • A twodimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining
    • Hayduk EJ, Choe LH, Lee KH (2004) A twodimensional electrophoresis map of Chinese hamster ovary cell proteins based on fluorescence staining. Electrophoresis 25:2545-56
    • (2004) Electrophoresis , vol.25 , pp. 2545-2556
    • Hayduk, E.J.1    Choe, L.H.2    Lee, K.H.3
  • 26
    • 8744300809 scopus 로고    scopus 로고
    • Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness
    • Ge Y, Rajkumar L, Guzman RC et al (2004) Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness. Proteomics 4:346
    • (2004) Proteomics , vol.4 , pp. 346
    • Ge, Y.1    Rajkumar, L.2    Guzman, R.C.3
  • 27
    • 84886603310 scopus 로고
    • Estimation of molecular weight by polyacrylamide gel electrophoresis using heat stable fl uorophors
    • Barger BO, White RC, Pace JL et al (1976) Estimation of molecular weight by polyacrylamide gel electrophoresis using heat stable fl uorophors. Anal Biochem 70:327-335
    • (1976) Anal Biochem , vol.70 , pp. 327-335
    • Barger, B.O.1    White, R.C.2    Pace, J.L.3
  • 28
    • 0024052825 scopus 로고
    • Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in thefirst-dimensional isoelectric focusing gel with the fl uorophore 2-methoxy-2,4-diphenyl-3(2H)furanone
    • Jackson P, Urwin VE, Mackay CD (1988) Rapid imaging, using a cooled charge-coupled-device, of fluorescent two-dimensional polyacrylamide gels produced by labelling proteins in thefirst-dimensional isoelectric focusing gel with the fl uorophore 2-methoxy-2,4-diphenyl-3(2H)furanone. Electrophoresis 9:330-339
    • (1988) Electrophoresis , vol.9 , pp. 330-339
    • Jackson, P.1    Urwin, V.E.2    Mackay, C.D.3
  • 29
    • 0035289178 scopus 로고    scopus 로고
    • Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology
    • Tonge R, Shaw J, Middleton B et al (2001) Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology. Proteomics 1:377-396
    • (2001) Proteomics , vol.1 , pp. 377-396
    • Tonge, R.1    Shaw, J.2    Middleton, B.3
  • 30
    • 0343376097 scopus 로고    scopus 로고
    • Difference gel electrophoresis: A single gel method for detecting changes in protein extracts
    • Uenlue M, Morgan ME, Minden JS (1997) Difference gel electrophoresis: a single gel method for detecting changes in protein extracts. Electrophoresis 18:2071-2077
    • (1997) Electrophoresis , vol.18 , pp. 2071-2077
    • Uenlue, M.1    Morgan, M.E.2    Minden, J.S.3
  • 31
    • 50849134572 scopus 로고    scopus 로고
    • Difference gel electrophoresis (DIGE): The next generation of two-dimensional gel electrophoresis for clinical research
    • In: Marcus K, Stuehler K, van Hall A, Hamacher M et al. (eds). Wiley-VCH, Weinheim
    • Sitek B, Scheibe B, Jung K, Schramm A, Stuehler K (2006) Difference gel electrophoresis (DIGE): the next generation of two-dimensional gel electrophoresis for clinical research. In: Marcus K, Stuehler K, van Hall A, Hamacher M et al. (eds) Proteomics in drug research. Wiley-VCH, Weinheim, pp 33-55
    • (2006) Proteomics in Drug Research , pp. 33-55
    • Sitek, B.1    Scheibe, B.2    Jung, K.3    Schramm, A.4    Stuehler, K.5
  • 32
    • 32944461534 scopus 로고    scopus 로고
    • Detection of phosphorylation patterns in rat cortical neurons by combining phosphatase treatment and DIGE technology
    • Raggiaschi R, Lorenzetto C, Diodato E et al (2006) Detection of phosphorylation patterns in rat cortical neurons by combining phosphatase treatment and DIGE technology. Proteomics 2006(6):748-756
    • (2006) Proteomics , Issue.6 , pp. 748-756
    • Raggiaschi, R.1    Lorenzetto, C.2    Diodato, E.3
  • 33
    • 0036463947 scopus 로고    scopus 로고
    • Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system
    • Gharbi S, Gaffney P, Yang A et al (2001) Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system. Mol Cell Proteomics 1:91-98
    • (2001) Mol Cell Proteomics , vol.1 , pp. 91-98
    • Gharbi, S.1    Gaffney, P.2    Yang, A.3
  • 34
    • 11244346557 scopus 로고    scopus 로고
    • Variance stabilization applied to microarray data calibration and to the quantification of differential expression
    • Huber W, von Heydebreck A, Sultmann H et al (2002) Variance stabilization applied to microarray data calibration and to the quantification of differential expression. Bioinformatics 18(Suppl 1):S96-104
    • (2002) Bioinformatics , vol.18 , Issue.SUPPL. 1
    • Huber, W.1    Von Heydebreck, A.2    Sultmann, H.3
  • 35
    • 2442453377 scopus 로고    scopus 로고
    • Determining a significant change in protein expression with DeCyder during a pair-wise comparison using two-dimensional difference gel electrophoresis
    • Karp NA, Kreil DP, Lilley KS (2004) Determining a significant change in protein expression with DeCyder during a pair-wise comparison using two-dimensional difference gel electrophoresis. Proteomics 4:1421-1432
    • (2004) Proteomics , vol.4 , pp. 1421-1432
    • Karp, N.A.1    Kreil, D.P.2    Lilley, K.S.3
  • 36
    • 4544270574 scopus 로고    scopus 로고
    • DNA microarray normalization methods can remove bias from differential protein expression analysis of 2D difference gel electrophoresis results
    • Kreil DP, Karp NA, Lilley KS (2004) DNA microarray normalization methods can remove bias from differential protein expression analysis of 2D difference gel electrophoresis results. Bioinformatics 20:2026-2034
    • (2004) Bioinformatics , vol.20 , pp. 2026-2034
    • Kreil, D.P.1    Karp, N.A.2    Lilley, K.S.3
  • 37
    • 27544487522 scopus 로고    scopus 로고
    • Statistical challenges in the analysis of two-dimensional difference gel electrophoresis experiments using DeCyder
    • Fodor IK, Nelson DO, Alegria-Hartman M et al (2005) Statistical challenges in the analysis of two-dimensional difference gel electrophoresis experiments using DeCyder. Bioinformatics 21:3733-3740
    • (2005) Bioinformatics , vol.21 , pp. 3733-3740
    • Fodor, I.K.1    Nelson, D.O.2    Alegria-Hartman, M.3
  • 38
    • 26044480351 scopus 로고    scopus 로고
    • Differential In-Gel Electrophoresis in a High-Throughput Environment
    • In: Walker JM (ed). Humana Press, Totowa, NJ
    • Somiari RI, Russell S, Somiari SB, Sullivan AG et al (2005) Differential In-Gel Electrophoresis in a High-Throughput Environment. In: Walker JM (ed) The proteomics handbook. Humana Press, Totowa, NJ, pp 223-237.
    • (2005) The Proteomics Handbook , pp. 223-237
    • Somiari, R.I.1    Russell, S.2    Somiari, S.B.3    Sullivan, A.G.4
  • 39
    • 18844447205 scopus 로고    scopus 로고
    • Saturation labeling with cysteinereactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser-microdissected clinical specimens
    • Greengauz-Roberts O, Stoppler H, Nomura S et al (2005) Saturation labeling with cysteinereactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser-microdissected clinical specimens. Proteomics 5:1746-1757
    • (2005) Proteomics , vol.5 , pp. 1746-1757
    • Greengauz-Roberts, O.1    Stoppler, H.2    Nomura, S.3
  • 40
    • 27144527588 scopus 로고    scopus 로고
    • Comparative proteomic analysis using samples obtained with laser microdissection and saturation dye labeling
    • Wilson KE, Marouga R, Prime JE et al (2005) Comparative proteomic analysis using samples obtained with laser microdissection and saturation dye labeling. Proteomics 5: 3851-3858
    • (2005) Proteomics , vol.5 , pp. 3851-3858
    • Wilson, K.E.1    Marouga, R.2    Prime, J.E.3
  • 41
    • 22044450210 scopus 로고    scopus 로고
    • Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma
    • Sitek B, Luttges J, Marcus K et al (2005) Application of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma. Proteomics 5: 2665-2679
    • (2005) Proteomics , vol.5 , pp. 2665-2679
    • Sitek, B.1    Luttges, J.2    Marcus, K.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.