Quantitative femto- to attomole immunodetection of regulated secretory vesicle proteins critical to exocytosis

Analytical Biochemistry

Volumn 307, Issue 1, 2002, Pages 54-62

  Coorssen, Jens R ^a,b   Blank, Paul S ^a   Albertorio, Fernando ^a   Bezrukov, Ludmila ^a   Kolosova, Irina ^a   Backlund Jr , Peter S ^a   Zimmerberg, Joshua ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b UNIVERSITY OF CALGARY   (Canada)

Author keywords

Protein purity; Quantitative immunodetection; Secretory vesicles; SNARE proteins; Western blotting

Indexed keywords

CELL MEMBRANES; MAMMALS;

IMMUNODETECTION; PROTEIN PURITY; SECRETORY VESICLES; SNARE PROTEINS; WESTERN BLOTTING;

PROTEINS;

CALCIUM; PROTEIN; SNARE PROTEIN; MEMBRANE PROTEIN; NERVE PROTEIN; SYNAPTOBREVIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN; VESICULAR TRANSPORT PROTEIN;

ACCURACY; ARTICLE; CONCENTRATION (PARAMETERS); EUKARYOTE; EXOCYTOSIS; HYPOTHESIS; IMMUNODETECTION; MEASUREMENT; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PURIFICATION; QUANTITATIVE ANALYSIS; SEA URCHIN; SECRETORY VESICLE; SURFACE PROPERTY; SYNAPSE VESICLE; TECHNIQUE; WESTERN BLOTTING; ANIMAL; CHEMISTRY; DRUG EFFECT; METABOLISM; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

ECHINOIDEA; EUKARYOTA;

ANIMALS; BLOTTING, WESTERN; CALCIUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXOCYTOSIS; MEMBRANE PROTEINS; NERVE TISSUE PROTEINS; QA-SNARE PROTEINS; R-SNARE PROTEINS; SEA URCHINS; SECRETORY VESICLES; SNARE PROTEINS; SYNAPTIC VESICLES; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; VESICULAR TRANSPORT PROTEINS;

EID: 0036667910     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00015-5     Document Type: Article

References (36)

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
2. "Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
3. Quantitative determination of growth hormone by immunoblotting
4. Quantitative measurement of proteins by western blotting with Cy5-coupled secondary antibodies
5. Determination of copy number of c-Myc protein per cell by quantitative Western blotting
6. Mechanisms of synaptic vesicle exocytosis
7. 2+sensitivity
8. 2+-triggered fusion steps of exocytosis: Rational and perspectives
9. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy
10. Sequential SNARE assembly underlies priming and triggering of exocytosis
11. SNAREpins: Minimal machinery for membrane fusion
12. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
13. Activity-dependent changes in partial VAMP complexes during neurotransmitter release
14. Calcium can disrupt the SNARE protein complex on sea urchin egg secretory vesicles without irreversibly blocking fusion
15. Are SNARE proteins necessary and sufficient for membrane fusion?
16. Modification of the Laemmli sodium dodecyl sulfate-polyacrylamide gel electrophoresis procedure to eliminate artefacts on reducing and nonreducing gels
17. Improved electrophoresis and transfer of picogram amounts of protein with hemoglobin
18. Calculation of protein extinction coefficients from amino acid sequence data
19. Blockade by botulinum neurotoxin B of catecholamine release from adrenochromaffin cells correlates with its cleavage of synaptobrevin and a homologue present on granules
20. Interpretation of electrophoretic band shapes by a partition chromatographic model
21. Mathematical functions for the representation of chromatographic peaks
22. On the transfer of polypeptides from gels to nitrocellulose membranes
23. High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis
24. The t-SNAREs syntaxin I and SNAP-25 are present on organelles that participate in synaptic vesicle recycling
25. Compartment specificity of cellular membrane fusion encoded in SNARE proteins
26. cis-Unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin
27. A kinetic and structural study of two-step aggregation and fusion of neutral phospholipid vesicles promoted by serum albumin at low pH
28. Clathrin-induced pH-dependent fusion of phosphatidylcholine vesicles
29. 2+-induced fusion of phosphatidate-containing membranes
30. Protein-mediated intermembrane contact facilitates fusion of lipid vesicles with planar bilayers
31. Defensins promote fusion and lysis of negatively charged membranes
32. Cytosolic ATPases, p97, and NSF, are sufficient to mediate rapid membrane fusion
33. Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors
34. Induction of aggregation and fusion of cholesterol-containing membrane vesicles by an anti-cholesterol monoclonal antibody