Constraint-based modeling of heterologous pathways: Application and experimental demonstration for overproduction of fatty acids in Escherichia coli

Biotechnology and Bioengineering

Volumn 111, Issue 10, 2014, Pages 2056-2066

  Ip, Kuhn ^a,b   Donoghue, Neil ^a   Kim, Min Kyung ^b   Lun, Desmond S ^a,b  

^a UNIVERSITY OF SOUTH AUSTRALIA   (Australia)

^b The State University of New Jersey   (United States)

Author keywords

Biofuel; Computational strain design; Constraint based modeling; Escherichia coli; Fatty acids overproduction

Indexed keywords

BIOFUELS; CARBON; FATTY ACIDS; FLOOD CONTROL; GENES; METABOLIC ENGINEERING;

CONSTRAINT-BASED MODELING; ESCHERICHIA COLI (E. COLI); EXPERIMENTAL DEMONSTRATIONS; FLUX BALANCE ANALYSIS; GENETIC MANIPULATIONS; LIQUID TRANSPORTATION; METABOLIC PHENOTYPE; STRAIN OPTIMIZATION;

ESCHERICHIA COLI;

BIODIESEL; CASAMINO ACID; FATTY ACID; FUEL; GASOLINE; THIOL ESTER HYDROLASE; ESCHERICHIA COLI PROTEIN;

ARTICLE; BACTERIUM MUTANT; CARBON SOURCE; CLINICAL EFFECTIVENESS; DNA MODIFICATION; ESCHERICHIA COLI; FATTY ACID ANALYSIS; FATTY ACID SYNTHESIS; GENE DELETION; GENETIC MANIPULATION; GENETIC MODEL; HETEROLOGOUS EXPRESSION; LIQUID; MATHEMATICAL GENETICS; MATHEMATICAL PHENOMENA; METABOLIC ENGINEERING; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PHENOTYPE; PROCESS OPTIMIZATION; PROPORTIONAL FLUX FORCING; TRANSPORT KINETICS; BACTERIAL GENE; BIOLOGICAL MODEL; COMPUTER SIMULATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE;

ESCHERICHIA COLI;

BASE SEQUENCE; COMPUTER SIMULATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FATTY ACIDS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENES, BACTERIAL; METABOLIC ENGINEERING; MODELS, BIOLOGICAL; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTATION;

EID: 84906784381     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25261     Document Type: Article

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