Molecular characterization of a novel hepcidin (HepcD) from Camelus dromedarius. Synthetic peptide forms exhibit antibacterial activity

Journal of Peptide Science

Volumn 20, Issue 9, 2014, Pages 680-688

  Boumaiza, Mohamed ^a   Ezzine, Aymen ^a   Jaouen, Maryse ^b   Sari, Marie Agnes ^b   Marzouki, Mohamed Nejib ^a  

^a UNIVERSITY OF CARTHAGE   (Tunisia)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

antimicrobial activity; Camelus dromedarius; hepcidin cDNA; hepcidin 25 analogs; MTT reduction test

Indexed keywords

CYSTEINE; HEPCIDIN; HEPCIDIN D; PROTEIN PRECURSOR; SYNTHETIC PEPTIDE; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; DISULFIDE;

AMINO ACID SEQUENCE; ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS SUBTILIS; BIOINFORMATICS; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOTOXICITY; DISULFIDE BOND; DROMEDARY; ESCHERICHIA COLI; INNATE IMMUNITY; LISTERIA MONOCYTOGENES; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS; STRUCTURAL HOMOLOGY; STRUCTURE ANALYSIS; ANIMAL; BACTERIA; CAMEL; CHEMISTRY; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; MOLECULAR CLONING; MOLECULAR GENETICS; SYNTHESIS;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIA; BASE SEQUENCE; CAMELS; CLONING, MOLECULAR; DISULFIDES; HEPCIDINS; HUMANS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES;

EID: 84905982676     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.2644     Document Type: Article

References (51)

1. Krause A, Neitz S, Magert HJ, Schulz A, Forssmann WG, Schulz KP, Adermann K,. LEAP-1: a novel highly disulfide bonded human peptide exhibits antimicrobial activity. FEBS Lett. 2000; 480: 147-150.
2. Park CH, Valore EV, Waring AJ, Ganz T,. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J. Biol. Chem. 2001; 276: 7806-7810.
3. Falzacappa MVV, Muckenthaler MU,. Hepcidin: iron-hormone and antimicrobial peptide. Gene 2005; 364: 37-44.
4. Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, Kahn A, Vaulont S,. The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J. Clin. Invest. 2002; 110: 1037-1044.
5. Jordan JB, Poppe L, Haniu M, Arvedson T, Syed R, Li V, Kohno H, Kim H, Schnier PD, Harvey TS, Miranda LP, Cheetham J, Sasu BJ,. Hepcidin revisited disulfide connectivity dynamics and structure. J. Biol. Chem. 2009; 284: 24155-24167.
6. Hunter HN, Fulton DB, Ganz T, Vogel HJ,. The solution structure of human hepcidin a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis. J. Biol. Chem. 2002; 277: 37597-37603.
7. Donovan A, Lima CA, Pinkus JL, Pinkus GS, Zon LI, Robine S, Andrews, NC,. The iron exporter ferroportin/Slc40a1 is essential for iron homeostasis. Cell Metab. 2005; 3: 191-200.
8. Nemeth E, Tuttle MS, Powelson J, Vaughn MB, Donovan A, Ward DM, Ganz T, Kaplan J,. Hepcidin regulates cellular iron efflux by binding to ferroportin and inducing its internalization. A.A.A.S. 2004; 306: 2090-2093.
9. Weinberg ED,. Cellular iron metabolism in health and disease. Drug Metab. Rev. 1990; 22: 531-579.
10. Bullen JJ, Rogers HJ, Spalding PB, Ward CG,. Iron and infection: the heart of the matter. FEMS Immunol. Med. Microbiol. 2005; 43: 325-330.
11. Wang KJ, Cai JJ, Cai L, Qu HD, Yang M, Zhang, M,. Cloning and expression of a hepcidin gene from a marine fish (Pseudosciaena crocea) and the antimicrobial activity of its synthetic peptide. Peptides 2009; 30: 638-646.
12. Maisetta G, Petruzzelli R, Brancatisanoa FL, Esin S, Vitali A, Campa M, Batoni G,. Antimicrobial activity of human hepcidin 20 and 25 against clinically relevant bacterial strains: effect of copper and acidic pH. Peptides 2010; 31: 1995-2002.
13. Zhou JG, Wei JG, Xu D, Cui HC, Yan Y, Ou-Yang ZL, Huang XH, Huang YH, Qin QW,. Molecular cloning and characterization of two novel hepcidins from orange-spotted grouper Epinephelus coioides. Fish Shellfish Immunol. 2011; 30: 559-568.
14. Verga FMV, Muckenthaler MU,. Hepcidin: iron-hormone and anti-microbial peptide. Gene 2005; 364: 37-44.
15. Rossi E., Hepcidin-the iron regulatory hormone. Clin. Biochem. Rev. 2005; 26: 47-49.
16. Maisetta G, Vitali A, Scorciapino MA, Rinaldi AC, Petruzelli R, Brancatisano FL, Esin S, Stringaro A, Colone M, Luzi C, Bozzi A, Campa M, Batoni G,. pH-dependent disruption of Escherichia coli ATCC 25922 and model membranes by the human antimicrobial peptides hepcidin 20 and 25. FEBS J. 2013; 280: 2842-2854.
17. Hocquellet A, Le Senechal C, Garbay B,. Importance of the disulfide bridges in the antibacterial activity of human hepcidin. Peptides 2012; 36: 303-307.
18. Liu F, Li JL, Yue GH, Fu JJ, Zhou ZF,. Molecular cloning and expression analysis of the liver-expressed antimicrobial peptide 2 (LEAP-2) gene in grass carp. Vet. Immunol. Immunopathol. 2010; 133: 133-143.
19. Khangembam VC, Kumar A,. Buffalo hepcidin: characterization of cDNA and study of antimicrobial property. Vet. Res. Commun. 2011; 35: 79-87.
20. Wilson RT,. The Camel, 1st edn, Longman Group Ltd.: Harlow, Essex, London, 1984.
21. Yagil R,. The desert camel: comparative physiological adaptation. In Comparative Animal Nutrition, Karger (ed.). Karger: Basel, Switzerland, 1985, 163.
22. Elrobh MS, Alanazi MS, Khan W, Abduljaleel Z, Al-Amri A, Bazzi MD,. Molecular cloning and characterization of cDNA encoding a putative stress-induced heat-shock protein from Camelus dromedarius. Int. J. Mol. Sci. 2011; 12: 4214-4236.
23. http://www.cbs.dtu.dk/services/SignalP/ [accessed August, 2013].
24. http://www.cbs.dtu.dk/services/ProP/ [accessed June, 2013].
25. Hall TA,. BioEdit: a user friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp. Ser. 1999; 41: 95-98.
26. Waterhouse AM, Procter JB, Martin DMA, Clamp M, Barton GJ,. Jalview Version 2-a multiple sequence alignment editor and analysis workbench. Bioinformatics 2009; 25: 1189-1191.
27. Saitou N, Nei M,. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 1987; 4: 406-425.
28. Tamura K, Dudley J, Nei M, Kumar S,. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007; 24: 1596-1599.
29. http://swissmodel.expasy.org/ [accessed October, 2013].
30. http://www.expasy.org/ [accessed October 2013].
31. DeLano WL,. The PyMOL Molecular Graphics System DeLano Scientific, San Carlos, CA, USA, 2002.
32. Khemtémourian L, Desbenoit N, Mahesh P, Chatterjee S, Deschemin JC, Vaulont S, Tomas A, Sari MA, Artaud I,. Synthesis and biological activity of mouse hepcidin peptide analogs containing three disulfide bridges: manual and microwave-assisted solid-phase peptide synthesis. Protein Pept. Lett. 2012; 19: 000-000.
33. Wang H, Cheng H, Wang F, Wei D, Wang X,. An improved 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells. J. Microbiol. Methods 2010; 82: 330-333.
34. http://www.statsoft.com/Products/STATISTICA/ [accessed January, 2014].
35. Hwang PM, Vogel H J,. Structure-function relationships of antimicrobial peptides. Biochem. Cell Biol. 1998; 76: 235-246.
36. Badial PR, Filho JPO, Cunha PHJ, Cagnini DQ, Araújo JJP, Winand NJ, Borges AS,. Identification characterization and expression analysis of hepcidin gene in sheep. Res. Vet. Sci. 2011; 90: 443-450.
37. Oliveira FJP, Badial PR, Paulo HJ, Cunha PHJ, Cruz TF, Araujo JJP, Divers TJ, Winand NJ, Borges AS,. Cloning sequencing and expression analysis of the equine hepcidin gene by real-time PCR. Vet. Immunol. Immunopathol. 2010; 135: 34-42.
38. Fry MM, Liggett JL, Baek SJ,. Molecular cloning and expression of canine hepcidin. Vet. Clin. Pathol. 2004; 33: 223-227.
39. Kim YO, Park EM, Nam BH, Kong HJ, Kim WJ, Lee SJ,. Identification and molecular characterization of two hepcidin genes from black rockfish (Sebastes schlegelii). Mol. Cell. Biochem. 2008; 315: 131-136.
40. Hao J, Li YW, Xie MQ, Li AX,. Molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from Simensis crocodile (Crocodylus siamensis). Comp. Biochem. Physiol. B 2012; 163: 309-315.
41. Nemeth E, Preza GC, Jung C, Kaplan J, Ganz T,. The N-terminus of hepcidin is essential for its interaction with ferroportin: structure function study. Blood 2006; 107: 328-333.
42. Gagliardo B, Faye A, Jaouen M, Deschemin JC, Cannone-Hergaux JF, Vaulont S, Sari MA,. Production of biologically active forms of recombinant hepcidin, the iron regulating hormone. FEBS J. 2008; 275: 3793-3803.
43. Hancock REW, Sahl HG,. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat. Biotechnol. 2006; 24: 1551-1557.
44. Brogden KA,. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nat. Rev. 2005; 3: 238-250.
45. Matsuzaki K, Nakayama M, Fukui M, Otaka A, Funakoshi S, Fujii N, Bessho K, Miyajima K,. Role of disulfide linkages in tachyplesin-lipid interactions. Biochemistry 1993; 43: 11704-11710.
46. Kawano K, Yoneya T, Miyata T, Yoshikawa K, Tokunaga F, Terada Y, Iwanaga S,. Antimicrobial peptide tachyplesin I isolated from hemocytes of the horseshoe crab (Tachypleus tridentatus). NMR determination of the beta-sheet structure. J. Biol. Chem. 1990; 265: 15365-15367.
47. Qureshi S, Kataria AK, Gahlot TK,. Bacterial microflora associated with wounds and abscesses on camel (Camelus dromedarius) skin. J. Camel Pract. Res. 2002; 9: 129-134.
48. Sandeep KS, Prerna N, Taruna B, Nazeer M, Sneha C, Rajneesh R, Sudeep S, Anil KK,. Characterization of staphylococcus aureus isolated from nasal discharge from pneumonic camels (Camelus dromedarius). ABAH Bioflux 2013; 5: 38-43.
49. Gaillot O, Pellegrini E, Bregenholt S, Nair S, Berche P,. The ClpP serine protease is essential for the intracellular parasitism and virulence of Listeria monocytogenes. Mol. Microbiol. 2000; 35: 1286-1294.
50. Charpentier E, Courvalin P,. Antibiotic resistance in Listeria spp. Antimicrob. Agents Chemother. 1999; 43: 2103-2108.
51. Morvan A, Moubareck C, Leclercq A, Hervé BM, Bremont S, Lecuit M, Courvalin P, Le Monnier A,. Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrob. Agents Chemother. 2010. DOI: 10.1128/AAC.01557-09.