Molecular cloning and characterization of cDNA encoding a putative stress-induced heat-shock protein from Camelus dromedarius

International Journal of Molecular Sciences

Volumn 12, Issue 7, 2011, Pages 4214-4236

  Elrobh, Mohamed S ^a   Alanazi, Mohammad S ^a   Khan, Wajahatullah ^a   Abduljalee, Zainularifeen ^a   Al Amri, Abdullah ^a   Bazzi, Mohammad D ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

3D structure; Alignment; Arabian camel; CDNA cloning; HSPA6; Molecular cloning; RACE; Real Time PCR; Sequence characterization

Indexed keywords

COMPLEMENTARY DNA; HEAT SHOCK PROTEIN; PROTEIN HSPA6; UNCLASSIFIED DRUG; HEAT SHOCK PROTEIN 70; RNA;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CAMEL; CAMELUS DROMEDARIUS; DNA SEQUENCE; GENE; GENE AMPLIFICATION; GENETIC CONSERVATION; HSPA6 GENE; MALE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PROTEIN STRUCTURE; SAUDI ARABIA; SEQUENCE HOMOLOGY; STRESS; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MOUSE; PHYLOGENY; PROTEIN TERTIARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

CAMELUS DROMEDARIUS; MAMMALIA;

ANIMALS; BASE SEQUENCE; CAMELS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; HSP70 HEAT-SHOCK PROTEINS; HUMANS; MALE; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PHYLOGENY; PROTEIN STRUCTURE, TERTIARY; RNA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, RNA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 79960808913     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12074214     Document Type: Article

References (57)

1. Pruski, A.M.; Dixon, D.R. Heat shock protein expression pattern (HSP70) in the hydrothermal vent mussel Bathymodiolus azoricus. Mar. Environ. Res. 2007, 64, 209-224.
2. Lilja, K.; Prevodnik, A.; Gardeström, J.; Elfwing, T.; Tedengren, M.; Bollner, T. Regional differences in mRNA responses in blue mussels within the Baltic proper. Comp. Biochem. Physiol. 2008, 148, 101-106.
3. Monari, M.; Foschi, J.; Rosmini, R.; Marin, M.G.; Serrazanetti, G.P. Heat shock protein 70 response to physical and chemical stress in Chamelea gallina. J. Exp. Mar. Biol. Ecol. 2011, 397, 71-78.
4. Hageman, J.; van Waarde, M.A.W.H.; Zylicz, A.; Walerych, D.; Kampinga, H.H. The diverse members of the mammalian HSP70 machine show distinct chaperone-like activities. Biochem. J. 2011, 435, 127-142.
5. Hammerer-Lercher, A.; Mair, J.; Bonatti, J.; Watzka, S.B.; Puschendorf, B.; Dirnhofer, S. Hypoxia induces heat shock protein expression in human coronary artery bypass grafts. Cardiovasc. Res. 2001, 50, 115-24.
6. Hochachka, P.W.; Somero, G.N. Biochemical Adaptation: Mechanism and Process in Physiological Evolution, 1st ed.; Oxford University Press: Oxford, MS, USA, 2002; p. 290.
7. Ackerman, P.A.; Forsyth, R.B.; Mazur, C.F.; Iwama, G.K. Stress hormones and the cellular stress response in salmonids. Fish Physiol. Biochem. 2000, 23, 327-336.
8. Bierkens, J.G.E.A. Applications and pitfalls of stress proteins in biomonitoring. Toxicology 2000, 153, 61-72.
9. Heldens, L.; Dirks, R.P.; Hensen, S.M.M.; Onnekink, C.; van Genesen, S.T.; Rustenburg, F.; Lubsen, N.H. Co-chaperones are limiting in a depleted chaperone network. Cell. Mol. Life Sci. 2010, 67, 4035-4048.
10. Leung, T.K.C.; Rajendran, M.Y.; Monfries, C.; Hall, C.; Lim, L. The human heat-shock protein family: Expression of a novel heat-inducible HSP70 (HSP7OB') and isolation of its cDNA and genomic DNA. Biochem. J. 1990, 267, 125-132.
11. Wada, K.; Taniguchi, A.; Xu, L.; Okano, T. Rapid and highly sensitive detection of cadmium chloride induced cytotoxicity using the HSP70B' promoter in live cells. Biotechnol. Bioeng. 2005, 92, 410-415.
12. Wang, X.Y.; Li, Y.; Yang, G.; Subjeck, J.R. Current ideas about applications of heat shock proteins in vaccine design and immunotherapy. Int. J. Hyperthermia 2005, 8, 717-722.
13. Xu, Q.; Schett, G.; Li, C.; Hu, Y.; Wick, G. Mechanical stress-induced heat shock protein 70 expression in vascular smooth muscle cells is regulated by Rac and Ras small G proteins but not mitogen-activated protein kinases. Circ. Res. 2000, 86, 1122-1128.
14. Romano, C.C.; Benedetto, N.; Catania, M.R.; Rizzo, A.; Gallè, F.; Losi, E.; Hasty, D.L.; Rossano, F. Commonly used antibiotics induce expression of Hsp 27 and Hsp 60 and protect human lymphocytes from apoptosis. Int. Immunopharmacol. 2004, 8, 1067-1073.
15. Noonan, E.J.; Place, R.F.; Giardina, C.; Hightower, L.E. Hsp70B' regulation and function. Cell Stress Chaperones 2007, 4, 393-402.
16. Parsian, A.J.; Sheren, J.E.; Tao, T.Y.; Goswami, P.C.; Malyapa, R.; Van Rheeden, R.; Watson, M.S.; Hunt, C.R. The human Hsp70B gene at the HSPA7 locus of chromosome 1 is transcribed but non-functional. Biochim. Biophys. Acta 2000, 1494, 201-205.
17. Fowler, M.E. Medicine and Surgery of Camelids, 3rd ed.; Wiley-Blackwell: Ames, IA, USA, 2010; pp. 4-15.
18. Khan, B.B.; Iqbal, A.; Riaz, M. Production and Management of Camels, 1st ed.; Pakistan T.M. Printers: Faisalabad, Pakistan, 2003.
19. Elkhawad, A.O. Selective brain cooling in desert animals: The Camel (Camelus dromedarius). Comp. Biochem. Physiol. 1992, 101, 195-202.
20. Thayyullathil, F.; Chathoth, S.; Hago, A.; Wernery, U.; Patel, M.; Galadari, S. Investigation of heat stress response in the camel fibroblast cell line dubca. Ann. N.Y. Acad. Sci. 2008, 1138, 376-384.
21. Kregel, K.C. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J. Appl. Physiol. 2002, 92, 2177-2186.
22. Leung, T.K.C.; Hall, C.; Rajendran, M.; Spurr, N.K.; Lim, L. The human heat-shock genes Hspa6 and Hspa7 are both expressed and localize to chromosome-1. Genomics 1992, 12, 74-79.
23. Gade, N.; Mahapatra, R.K.; Sonawane, A.; Singh, V.K.; Doreswamy, R.; Saini, M. Molecular Characterization of Heat Shock Protein 70-1 Gene of Goat (Capra hircus). Mol. Biol. Int. 2010, DOI:10.4061/2010/108429.
24. Phelham, H.R.B. A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene. Cell 1982, 30, 517-528.
25. Gutierrez, J.A.; Guerriero, V., Jr. Chemical modicfications of a recombinant bovine stress-inducible 70 kDa heat-shock protein (Hsp 70) mimics Hsp70 isoforms from tissues. Biochem. J. 1995, 305, 197-203.
26. Kano, R.; Abe, K.; Hasegawa, A. cDNA of canine heat shock protein 70 (HSP70). Vet. Res. Commun. 2004, 28, 395-405.
27. Morimoto, R.I.; Hunt, C.; Huang, S.Y.; Berg, L.; Banerji, S. Organization, nucleotide sequence and transcription of the chicken HSP70 gene. J. Biol. Chem. 1986, 261, 12692-12699.
28. Rensing, S.A.; Maier, U.G. Phylogenetic analysis of the stress-70 protein family. J. Mol. Evol. 1994, 39, 80-86.
29. Allen, R.L.; O'Brien, D.A.; Jones, C.C.; Rockett, D.L.; Eddy, E.M. Expression of heat shock proteins by isolated mouse spermatogenic cells. Mol. Cell. Biol. 1988, 8, 3260-3266.
30. White, C.N.; Hightower, L.E.; Schultz, R.J. Variation in heat-shock proteins among species of desert fishes (Poeciliidae, poecilliopsis). Mol. Biol. Evol. J. 1994, 1, 106-119.
31. Norris, C.E.; di Iorio, P.J.; Schultz, R.J.; Hightower, L.E. Variation in heat shock proteins within tropical and desert species of Poeciliid fishes. Mol. Biol. Evol. 1995, 6, 1048-1062.
32. Tavaria, M.; Gabriele, T.; Kola, I.; Anderson, R.L. A hitchhiker's guide to the human Hsp70 family. Cell Stress Chaperones 1996, 1, 23-28.
33. Manzerra, P.; Rush, S.J.; Brown, I.R. Tissue-specific differences in heat shock protein HSP70 and HSP70 in the control and hyperthermic rabbit. J. Cell. Physiol. 1997, 170, 130-137.
34. Place, S.P.; Hofmann, G.E. Comparison of Hsc70 orthologs from polar and temperate notothenioid fishes: differences in prevention of aggregation and refolding of denatured proteins. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2005, 288, R1195-R11202.
35. Yamashita, M.; Hirayoshi, K.; Nagata, K. Characterization of multiple members of the HSP70 family in platyfish culture cells: Molecular evolution of stress protein HSP70 in vertebrates. Gene 2004, 336, 207-218.
36. Hightower, L.E.; Norris, C.E.; di Iorio, P.J.; Fielding, E. Heat shock responses of closely related species of tropical and desert fish. Integr. Comp. Biol. 1999, 39, 877-888.
37. Mahmood, T.; Safdar, W.; Abbasi, B.H.; Naqvi, S.M. Overview on the small heat shock proteins. African J. Biotechnol. 2010, 9, 927-949.
38. Noonan, E.; Giardinaa, C.; Hightower, L. Hsp70B′ and Hsp72 form a complex in stressed human colon cells and each contributes to cytoprotection. Exp. Cell Res. 2008, 314, 2468-2476.
39. Chenchik, A.; Moqadam, F.; Siebert, P. A New Method for Full-Length cDNA Cloning by PCR. In A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis, 1st ed.; Krieg, P.A., Ed.; Wiley-Liss: New York, NY, USA 1996; pp. 273-321.
40. Expasy Translate Tool. Available online: http://www.expasy.ch/tools/dna.html (accessed on 24 June 2011).
41. Bioedit. Available online: http://www.mbio.ncsu.edu/bioedit/bioedit.html (accessed on 24 June 2011).
42. NCBI BLAST program. Available online: http://www.ncbi.nlm.nih.gov/blast (accessed on 24 June 2011).
43. CLC Genome workbench version 6.0.1. Available online: http://www.clcbio.com (accessed on 24 June 2011).
44. Protein Calculator v3.3. Available online: http://www.scripps.edu/~cdputnam/protcalc.html (accessed on 24 June 2011).
45. Guindon, S.; Gascuel, O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 2003, 52, 696-704.
46. Dereeper, A.; Audic, S.; Claverie, J.M.; Blanc, G. BLAST-EXPLORER helps you building datasets for phylogenetic analysis. BMC Evol. Biol. 2010, 10, 8.
47. Dereeper, A.; Guignon, V.; Blanc, G.; Audic, S.; Buffet, S.; Chevenet, F.; Dufayard, J.F.; Guindon, S.; Lefort, V.; Lescot, M.; et al. Phylogeny.fr: Robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008, 36, W465-W469.
48. Anisimova, M.; Gascuel, O. Approximate likelihood ratio test for branches: A fast, accurate and powerful alternative. Syst. Biol. 2006, 55, 539-552.
49. Eisenberg, D.; Schwarz, E.; Komaromy, M.; Wall, R. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 1984, 179, 125-142.
50. Eswar, N.; Eramian, D.; Webb, B.; Shen, M.; Sali, A. Protein Structure Modeling With MODELLER. Methods Mol. Biol. 2006, 426, 145-159.
51. Marti-Renom, M.A.; Madhusudhan, M.S.; Sali, A. Alignment of protein sequences by their profiles. Protein Sci. 2004, 13, 1071-1087.
52. Edgar, R.C. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res.2004, 32, 1792-1797.
53. Roy, A.; Kucukural, A.; Zhang, Y. I-TASSER: A unified platform for automated protein structure and function prediction. Nat. Protoc. 2010, 5, 725-738.
54. Zhang, Y. I-TASSER server for protein 3D structure prediction. BMC Bioinfma. 2008, 9, 40.
55. Benkert, P.; Künzli, M.; Schwede, T. QMEAN server for protein model quality estimation. Nucleic Acids Res. 2009, 37, W510-W514.
56. Benkert, P.; Biasini, M.; Schwede, T. Toward the estimation of the absolute quality of individual protein structure models. Bioinformatics 2011, 27, 343-350.
57. Holm, L.; Park, J. DaliLite workbench for protein structure comparison. Bioinformatics 2000, 16, 566-567.