Planctomycetes and macroalgae, a striking association

Frontiers in Microbiology

Volumn 5, Issue JUN, 2014, Pages

  Lage, Olga M ^a,b   Bondoso, Joana ^a,b  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

Author keywords

Association; Biofilm; Macroalgae; Macroalgae exudates; Planctomycetes

Indexed keywords

POLYSACCHARIDE;

ALGAL CELL CULTURE; BACTERIAL COLONIZATION; BIOFILM; CELL WALL; ENVIRONMENTAL FACTOR; FLUORESCENCE IN SITU HYBRIDIZATION; GENE LIBRARY; GENE SEQUENCE; MACROALGA; MICROBIAL COMMUNITY; MICROENVIRONMENT; NONHUMAN; NUCLEOTIDE SEQUENCE; ORGANISMAL INTERACTION; PHYLOGENETIC TREE; PHYLOGENY; PLANCTOMYCETES; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; UNINDEXED SEQUENCE;

EID: 84905198664     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2014.00267     Document Type: Review

References (83)

1. Abed, R. M. M., Al Kharusi, S., Schramm, A., and Robinson, M. D. (2010). Bacterial diversity, pigments and nitrogen fixation of biological desert crusts from the Sultanate of Oman. FEMS Microbiol. Ecol. 72, 418-428. doi: 10.1111/j.1574-6941.2010.00854.x
2. Abed, R. M. M., Musat, N., Musat, F., and Mußmann, M. (2011). Structure of microbial communities and hydrocarbon-dependent sulfate reduction in the anoxic layer of a polluted microbial mat. Mar. Pollut. Bull. 62, 539-546. doi: 10.1016/j.marpolbul.2010.11.030
3. Akob, D. M., Mills, H. J., and Kostka, J. E. (2007). Metabolically active microbial communities in uranium-contaminated subsurface sediments. FEMS Microbiol. Ecol. 59, 95-107. doi: 10.1111/j.1574-6941.2006.00203.x
4. Andrew, D. R., Fitak, R. R., Munguia-Vega, A., Racolta, A., Martinson, V. G., and Dontsova, K. (2012). Abiotic factors shape microbial diversity in Sonoran desert soils. Appl. Environ. Microbiol. 78, 7527-7537. doi: 10.1128/AEM.01459-12
5. Armstrong, E., Yan, L., Boyd, K. G., Wright, P. C., and Burgess, J. G. (2001). The symbiotic role of marine microbes on living surfaces. Hydrobiologia 461, 37-40. doi: 10.1023/A:1012756913566
6. Baumgartner, L. K., Dupraz, C., Buckley, D. H., Spear, J. R., Pace, N. R., and Visscher, P. T. (2009). Microbial species richness and metabolic activities in hypersaline microbial mats: insight into biosignature formation through lithification. Astrobiology 9, 861-874. doi: 10.1089/ast.2008.0329
7. Bengtsson, M. M., and Ovreas, L. (2010). Planctomycetes dominate biofilms on surfaces of the kelp Laminaria hyperborea. BMC Microbiol. 10:261. doi: 10.1186/1471-2180-10-261
8. Bengtsson, M. M., Sjotun, K., Lanzen, A., and Ovreas, L. (2012). Bacterial diversity in relation to secondary production and succession on surfaces of the kelp Laminaria hyperborea. ISME J. 6, 2188-2198. doi: 10.1038/ismej.2012.67
9. Bengtsson, M. M., Sjotun, K., and Ovreås, L. (2010). Seasonal dynamics of bacterial biofilms on the kelp Laminaria hyperborea. Aquat. Microb. Ecol. 60, 71-83. doi: 10.3354/ame01409
10. Bjelland, T., Grube, M., Hoem, S., Jorgensen, S. L., Daae, F. L., Thorseth, I. H., et al. (2011). Microbial metacommunities in the lichen-rock habitat. Environ. Microbiol. Rep. 3, 434-442. doi: 10.1111/j.1758-2229.2010.00206.x
11. Bohorquez, L. C., Delgado-Serrano, L., Lopez, G., Osorio-Forero, C., Klepac-Ceraj, V., Kolter, R., et al. (2012). In-depth characterization via complementing culture-independent approaches of the microbial community in an acidic hot spring of the Colombian Andes. Microb. Ecol. 63, 103-115. doi: 10.1007/s00248-011-9943-3
12. Bondoso, J., Albuquerque, L., Lobo-Da-Cunha, A., Da Costa, M. S., Harder, J., and Lage, O. M. (2014). Rhodopirellula lusitana sp. nov. and Rhodopirellula rubra sp. nov., isolated from the surface of macroalgae. Syst. Appl. Microbiol. doi: 10.1016/j.syapm.2013.11.004
13. Bondoso, J., Balagué, V., Gasol, J. M., and Lage, O. M. (2013). Community composition of the Planctomycetes associated with different macroalgae. FEMS Microbiol. Ecol. doi: 10.1111/1574-6941.12258
14. Burke, C., Thomas, T., Lewis, M., Steinberg, P., and Kjelleberg, S. (2011). Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis. ISME J. 5, 590-600. doi: 10.1038/ismej.2010.164
15. Campbell, A. H., Marzinelli, E. M., Vergés, A., Coleman, M. A., and Steinberg, P. D. (2014). Towards restoration of missing underwater forests. PLoS ONE 9:e84106. doi: 10.1371/journal.pone.0084106
16. Caracciolo, A. B., Grenni, P., Ciccoli, R., Di Landa, G., and Cremisini, C. (2005). Simazine biodegradation in soil: analysis of bacterial community structure by in situ hybridization. Pest Manag. Sci. 61, 863-869. doi: 10.1002/ps.1096
17. Cayrou, C., Raoult, D., and Drancourt, M. (2010). Broad-spectrum antibiotic resistance of Planctomycetes organisms determined by Etest. J. Antimicrob. Chemother. 65, 2119-2122. doi: 10.1093/jac/dkq290
18. Chouari, R., Le Paslier, D., Daegelen, P., Ginestet, P., Weissenbach, J., and Sghir, A. (2003). Molecular evidence for novel planctomycete diversity in a municipal wastewater treatment plant. Appl. Environ. Microbiol. 69, 7354-7363. doi: 10.1128/AEM.69.12.7354-7363.2003
19. Costa, R., Keller-Costa, T., Gomes, N. C. M., Da Rocha, U. N., van Overbeek, L., and van Elsas, J. D. (2013). Evidence for selective bacterial community structuring in the freshwater sponge Ephydatia fluviatilis. Microb. Ecol. 65, 232-244. doi: 10.1007/s00248-012-0102-2
20. De Oliveira, L. S., Gregoracci, G. B., Silva, G. G., Salgado, L. T., Filho, G. A., Alves-Ferreira, M., et al. (2012). Transcriptomic analysis of the red seaweed Laurencia dendroidea (Florideophyceae, Rhodophyta) and its microbiome. BMC Genomics 13:487. doi: 10.1186/1471-2164-13-487
21. DeLong, E. F., Franks, D. G., and Alldredge, L. (1993). Phylogenetic diversity of aggregate-attached vs. free-living marine bacterial assemblages. Limnol. Oceanogr. 38, 924-934. doi: 10.4319/lo.1993.38.5.0924
22. Devos, D. P., and Reynaud, E. G. (2010). Intermediate steps. Science 330, 1187-1188. doi: 10.1126/science.1196720
23. Donadio, S., Monciardini, P., and Sosio, M. (2007). Polyketide synthases and nonribosomal peptide synthetases: the emerging view from bacterial genomics. Nat. Prod. Rep. 24, 1073-1109. doi: 10.1039/b514050c
24. Duque-Alarcón, A., Santiago-Vázquez, L. Z., and Kerr, R. G. (2012). A microbial community analysis of the octocoral Eunicea fusca. Electron. J. Biotechnol. 15, 1-9. doi: 10.2225/vol15-issue5-fulltext-11
25. Erbilgin, O., McDonald, K. L., and Kerfeld, C. A. (2014). Characterization of a planctomycetal organelle: a novel bacterial microcompartment for the aerobic degradation of plant saccharides. Appl. Environ. Microbiol. 80, 2193-2205. doi: 10.1128/AEM.03887-13
26. Fuerst, J. A., Gwilliam, H. G., Lindsay, M., Lichanska, A., Belcher, C., Vickers, J. E., et al. (1997). Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon. Appl. Environ. Microbiol. 63, 254-262.
27. Fuerst, J. A., and Sagulenko, E. (2013). Nested bacterial boxes: nuclear and other intracellular compartments in plactomycetes. J. Mol. Microbiol. Biotechnol. 23, 95-103. doi: 10.1159/000346544
28. Fukunaga, Y., Kurahashi, M., Sakiyama, Y., Ohuchi, M., Tokota, A., and Harayama, S. (2009). Phycisphaera mikurensis gen. nov., sp. nov., isolated from a marine alga, and proposal of phycisphaeraceae fam. nov., phycisphaerales ord. nov. and phycisphaerae classis nov. in the phylum planctomycetes. J. Gen. Appl. Microbiol. 55, 267-275. doi: 10.2323/jgam.55.267
29. Gade, D., Stuhrmann, T., Reinhardt, R., and Rabus, R. (2005). Growth phase dependent regulation of protein composition in Rhodopirellula baltica. Environ. Microbiol. 7, 1074-1084. doi: 10.1111/j.1462-2920.2005.00784.x
30. Glockner, F. O., Kube, M., Bauer, M., Teeling, H., Lombardot, T., Ludwig, W., et al. (2003). Complete genome sequence of the marine planctomycete Pirellula sp. strain 1. Proc. Natl. Acad. Sci. U. S. A. 100, 8298-8303. doi: 10.1073/pnas.1431443100
31. Goecke, F., Thiel, V., Wiese, J., Labes, A., and Imhoff, J. F. (2013). Algae as an important environment for bacteria-phylogenetic relationships among new bacterial species isolated from algae. Phycologia 52, 14-24. doi: 10.2216/12-24.1
32. Grube, M., Köberl, M., Lackner, S., Berg, C., and Berg, G. (2012). Host-parasite interaction and microbiome response: effects of fungal infections on the bacterial community of the Alpine lichen Solorina crocea. FEMS Microbiol. Ecol. 82, 472-481. doi: 10.1111/j.1574-6941.2012.01425.x
33. Guppy, R., and Bythell, J. C. (2006). Environmental effects on bacterial diversity in the surface mucus layer of the reef coral Montastraea faveolata. Mar. Ecol. Prog. Ser. 328, 133-142. doi: 10.3354/meps328133
34. Halter, D., Cordi, A., Gribaldo, S., Gallien, S., Goulhen-Chollet, F., Heinrich-Salmeron, A., et al. (2011). Taxonomic and functional prokaryote diversity in mildly arsenic-contaminated sediments. Res. Microbiol. 162, 878-887. doi: 10.1016/j.resmic.2011.06.001
35. Hempel, M., Blume, M., Blindow, I., and Gross, E. M. (2008). Epiphytic bacterial community composition on two common submerged macrophytes in brackish water and freshwater. BMC Microbiol. 8:58. doi: 10.1186/1471-2180-8-58
36. Hengst, M. B., Andrade, S., Gonzalez, B., and Correa, J. A. (2010). Changes in epiphytic bacterial communities of intertidal seaweeds modulated by host, temporality, and copper enrichment. Microb. Ecol. 60, 282-290. doi: 10.1007/s00248-010-9647-0
37. Hollants, J., Leliaert, F., De Clerck, O., and Willems, A. (2013). What we can learn from sushi: a review on seaweed-bacterial associations. FEMS Microbiol. Ecol. 83, 1-16. doi: 10.1111/j.1574-6941.2012.01446.x
38. Huang, Y., Niu, B., Gao, Y., Fu, L., and Li, W. (2010). CD-HIT Suite: a web server for clustering and comparing biological sequences. Bioinformatics 26, 680-682. doi: 10.1093/bioinformatics/btq003
39. Ivanova, A. O., and Dedysh, S. N. (2012). Abundance, diversity, and depth distribution of Planctomycetes in acidic northern wetlands. Front. Microbiol. 3:5. doi: 10.3389/fmicb.2012.00005
40. Jensen, S. I., Kühl, M., and Priemé, A. (2007). Different bacterial communities associated with the roots and bulk sediment of the seagrass Zostera marina. FEMS Microbiol. Ecol. 62, 108-117. doi: 10.1111/j.1574-6941.2007.00373.x
41. Jeske, O., Jogler, M., Petersen, J., Sikorski, J., and Jogler, C. (2013). From genome mining to phenotypic microarrays: Planctomycetes as source for novel bioactive molecules. Antonie Van Leeuwenhoek 104, 551-567. doi: 10.1007/s10482-013-0007-1
42. Kulichevskaia, I. S., Pankratov, T. A., and Dedysh, S. N. (2006). Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation methods. Mikrobiologiia. 75, 389-396.
43. Lachnit, T., Blümel, M., Imhoff, J. F., and Wahl, M. (2009). Specific epibacterial communities on macroalgae: phylogeny matters more than habitat. Aquatic Biology 5, 181-186. doi: 10.3354/ab00149
44. Lachnit, T., Meske, D., Wahl, M., Harder, T., and Schmitz, R. (2011). Epibacterial community patterns on marine macroalgae are host-specific but temporally variable. Environ. Microbiol. 13, 655-665. doi: 10.1111/j.1462-2920.2010.02371.x
45. Lage, O. (2013). Characterization of a planctomycete associated with the marine dinoflagellate Prorocentrum micans Her. Antonie Van Leeuwenhoek 104, 499-508. doi: 10.1007/s10482-013-9991-4
46. Lage, O., Bondoso, J., and Lobo-Da-Cunha, A. (2013). Insights into the ultrastructural morphology of novel Planctomycetes. Antonie van Leeuwenhoek, 1-10. doi: 10.1007/s10482-013-9969-2
47. Lage, O. M., and Bondoso, J. (2011). Planctomycetes diversity associated with macroalgae. FEMS Microbiol. Ecol. 78, 366-375. doi: 10.1111/j.1574-6941.2011.01168.x
48. Liu, M., Dong, Y., Zhao, Y., Zhang, G., Zhang, W., and Xiao, T. (2010). Structures of bacterial communities on the surface of Ulva prolifera and in seawaters in an Ulva blooming region in Jiaozhou Bay, China. World J. Microbiol. Biotechnol. 27, 1703-1712. doi: 10.1007/s11274-010-0627-9
49. Liu, Y., Yao, T., Jiao, N., Kang, S., Zeng, Y., and Huang, S. (2006). Microbial community structure in moraine lakes and glacial meltwaters, Mount Everest. FEMS Microbiol. Lett. 265, 98-105. doi: 10.1111/j.1574-6968.2006.00477.x
50. Longford, S. R., Tujula, N. A., Crocetti, G. R., Holmes, A. J., Holmström, C., Kjelleberg, S., et al. (2007). Comparisons of diversity of bacterial communities associated with three sessile marine eukaryotes. Aquat. Microb. Ecol. 48, 217-229. doi: 10.3354/ame048217
51. Lonhienne, T. G., Sagulenko, E., Webb, R. I., Lee, K. C., Franke, J., Devos, D. P., et al. (2010). Endocytosis-like protein uptake in the bacterium Gemmata obscuriglobus. Proc. Natl. Acad. Sci. U.S.A. 107, 12883-12888. doi: 10.1073/pnas.1001085107
52. Lucheta, A. R., Otero, X. L., Macías, F., and Lambais, M. R. (2013). Bacterial and archaeal communities in the acid pit lake sediments of a chalcopyrite mine. Extremophiles 17, 941-951. doi: 10.1007/s00792-013-0576-y
53. Matsuo, Y., Imagawa, H., Nishizawa, M., and Shizuri, Y. (2005). Isolation of an algal morphogenesis inducer from a marine bacterium. Science 307, 1598. doi: 10.1126/science.1105486
54. Meusnier, I., Olsen, J. L., Stam, W. T., Destombe, C., and Valero, M. (2001). Phylogenetic analyses of Caulerpa taxifolia (Chlorophyta) and of its associated bacterial microflora provide clues to the origin of the Mediterranean introduction. Mol. Ecol. 10, 931-946. doi: 10.1046/j.1365-294X.2001.01245.x
55. Miranda, L. N., Hutchison, K., Grossman, A. R., and Brawley, S. H. (2013). Diversity and abundance of the bacterial community of the red macroalga Porphyra umbilicalis: did bacterial farmers produce macroalgae? PLoS ONE 8:e58269. doi: 10.1371/journal.pone.0058269
56. Newsham, K. K., Pearce, D. A., and Bridge, P. D. (2010). Minimal influence of water and nutrient content on the bacterial community composition of a maritime Antarctic soil. Microbiol. Res. 165, 523-530. doi: 10.1016/j.micres.2009.11.005
57. Oliveira, F.a.S., Colares, G. B., Hissa, D. C., Angelim, A. L., Melo, V. M. M., and Lotufo, T.M.C. (2013). Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes sp. Symbiosis 59, 57-63. doi: 10.1007/s13199-012-0210-2
58. Pilhofer, M., Rappl, K., Eckl, C., Bauer, A. P., Ludwig, W., Schleifer, K. H., et al. (2008). Characterization and evolution of cell division and cell wall synthesis genes in the bacterial phyla Verrucomicrobia, Lentisphaerae, Chlamydiae, and Planctomycetes and phylogenetic comparison with rRNA genes. J. Bacteriol. 190, 3192-3202. doi: 10.1128/JB.01797-07
59. Pimentel-Elardo, S., Wehrl, M., Friedrich, A. B., Jensen, P. R., and Hentschel, U. (2003). Isolation of planctomycetes from Aplysina sponges. Aquat. Microb. Ecol. 33, 239-245. doi: 10.3354/ame033239
60. Piquet, A. M. T., Bolhuis, H., Davidson, A. T., and Buma, A. G. J. (2010). Seasonal succession and UV sensitivity of marine bacterioplankton at an Antarctic coastal site. FEMS Microbiol. Ecol. 73, 68-82. doi: 10.1111/j.1574-6941.2010.00882.x
61. Reed, D. W., Fujita, Y., Delwiche, M. E., Blackwelder, D. B., Sheridan, P. P., Uchida, T., et al. (2002). Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin. Appl. Environ. Microbiol. 68, 3759-3770. doi: 10.1128/AEM.68.8.3759-3770.2002
62. Reynaud, E. G., and Devos, D. P. (2011). Transitional forms between the three domains of life and evolutionary implications. Proc. Biol. Sci. 278, 3321-3328. doi: 10.1098/rspb.2011.1581
63. Rusch, D. B., Halpern, A. L., Sutton, G., Heidelberg, K. B., Williamson, S., Yooseph, S., et al. (2007). The sorcerer II global ocean sampling expedition: northwest atlantic through eastern tropical pacific. PLoS Biol. 5:e77. doi: 10.1371/journal.pbio.0050077
64. Santarella-Mellwig, R., Franke, J., Jaedicke, A., Gorjanacz, M., Bauer, U., Budd, A., et al. (2010). The compartmentalized Bacteria of the Planctomycetes-Verrucomicrobia-Chlamydiae superphylum have membrane coat-like proteins. PLoS Biol. 8:e1000281. doi: 10.1371/journal.pbio.1000281
65. Santarella-Mellwig, R., Pruggnaller, S., Roos, N., Mattaj, I. W., and Devos, D. P. (2013). Three-dimensional reconstruction of bacteria with a complex endomembrane system. PLoS Biol. 11:e1001565. doi: 10.1371/journal.pbio.1001565
66. Schlesner, H. (1994). The development of media suitable for the microorganisms morphologically resembling Planctomyces spp., Pirellula spp., and other Planctomycetales from various aquatic habitats using dilute media. Syst. Appl. Microbiol. 17, 135-145. doi: 10.1016/S0723-2020(11)80042-1
67. Schneider, D., Arp, G., Reimer, A., Reitner, J., and Daniel, R. (2013). Phylogenetic analysis of a microbialite-forming microbial mat from a hypersaline lake of the kiritimati atoll, central pacific. PLoS ONE 8:e66662. doi: 10.1371/journal.pone.0066662
68. Stackebrandt, E. (2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 52, 1043-1047. doi: 10.1099/ijs.0.02360-0
69. Tekere, M., Lötter, A., Olivier, J., Jonker, N., and Venter, S. (2011). Metagenomic analysis of bacterial diversity of siloam hot water spring, limpopo, South Africa. Afr. J. Biotechnol. 10, 18005-18012. doi: 10.5897/AJB11.899
70. Van Teeseling, M. C. F., Neumann, S., and Van Niftrik, L. (2013). The anammoxosome organelle is crucial for the energy metabolism of anaerobic ammonium oxidizing bacteria. J. Mol. Microbiol. Biotechnol. 23, 104-117. doi: 10.1159/000346547
71. Wahl, M. (2008). Ecological lever and interface ecology: epibiosis modulates the interactions between host and environment. Biofouling 24, 427-438. doi: 10.1080/08927010802339772
72. Ward, N., Staley, J. T., Fuerst, J. A., Giovannoni, S., Schlesner, H., and Stackebrandt, E. (2006). "The order Planctomycetales, including the genera Planctomyces, Pirellula, Gemmata and Isosphaera and the Candidatus genera Brocadia, Kuenenia and Scalindua," in The Prokaryotes: A Handbook on the Biology of Bacteria, Vol. 7, eds M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer, and E. Stackebrandt (New York, NY: Springer), 757-793.
73. Webster, N. S., and Bourne, D. (2007). Bacterial community structure associated with the Antarctic soft coral, Alcyonium antarcticum. FEMS Microbiol. Ecol. 59, 81-94. doi: 10.1111/j.1574-6941.2006.00195.x
74. Webster, N. S., Wilson, K. J., Blackall, L. L., and Hill, R. T. (2001). Phylogenetic diversity of bacteria associated with the marine sponge Rhopaloeides odorabile. Appl. Environ. Microbiol. 67, 434-444. doi: 10.1128/AEM.67.1.434-444.2001
75. Wecker, P., Klockow, C., Ellrott, A., Quast, C., Langhammer, P., Harder, J., et al. (2009). Transcriptional response of the model Planctomycete Rhodopirellula baltica SH1(T) to changing environmental conditions. BMC Genomics 10:410. doi: 10.1186/1471-2164-10-410
76. Wegner, C. E., Richter-Heitmann, T., Klindworth, A., Klockow, C., Richter, M., Achstetter, T., et al. (2013). Expression of sulfatases in Rhodopirellula baltica and the diversity of sulfatases in the genus Rhodopirellula. Mar. Genomics 9, 51-61. doi: 10.1016/j.margen.2012.12.001
77. Winkelmann, N., and Harder, J. (2009). An improved isolation method for attached-living Planctomycetes of the genus Rhodopirellula. J. Microbiol. Methods 77, 276-284. doi: 10.1016/j.mimet.2009.03.002
78. Yakimov, M. M., Cappello, S., Crisafi, E., Tursi, A., Savini, A., Corselli, C., et al. (2006). Phylogenetic survey of metabolically active microbial communities associated with the deep-sea coral Lophelia pertusa from the Apulian plateau, Central Mediterranean Sea. Deep-Sea Res. I 53, 62-75. doi: 10.1016/j.dsr.2005.07.005
79. Yoon, J., Jang, J. H., and Kasai, H. (2014). Algisphaera agarilytica gen. nov., sp. nov., a novel representative of the class Phycisphaerae within the phylum Planctomycetes isolated from a marine alga. Antonie Van Leeuwenhoek 105, 317-324. doi: 10.1007/s10482-013-0076-1
80. Zeng, Y. X., Yan, M., Yu, Y., Li, H. R., He, J. F., Sun, K., et al. (2013). Diversity of bacteria in surface ice of Austre Lovénbreen glacier, Svalbard. Arch. Microbiol. 195, 313-322. doi: 10.1007/s00203-013-0880-z
81. Zhang, W., Wu, X., Liu, G., Chen, T., Zhang, G., Dong, Z., et al. (2013). Pyrosequencing reveals bacterial diversity in the rhizosphere of three phragmites australis ecotypes. Geomicrobiol. J. 30, 593-599. doi: 10.1080/01490451.2012.740145
82. Zhao, Z., Luo, K., Chen, G., Yang, Y., Mao, Z., Liu, E., et al. (2010). Analysis of bacterial diversity in rhizosphere of cucumber in greenhouse by the methods of metagenomic end-random sequencing and 16S rDNA technology. Shengtai Xuebao/ Acta Ecologica Sinica 30, 3849-3857.
83. Zhu, P., Li, Q., and Wang, G. (2008). Unique microbial signatures of the alien hawaiian marine sponge Suberites zeteki. Microb. Ecol. 55, 406-414. doi: 10.1007/s00248-007-9285-3