Production of plant sesquiterpenes in Saccharomyces cerevisiae: Effect of ERG9 repression on sesquiterpene biosynthesis

Biotechnology and Bioengineering

Volumn 99, Issue 3, 2008, Pages 666-677

  Asadollahi, Mohammad A ^a   Maury, Jérôme ^a   Møller, Kasper ^a,c   Nielsen, Kristian Fog ^a   Schalk, Michel ^b   Clark, Anthony ^b   Nielsen, Jens ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b Analysis and Perception Department   (Switzerland)

^c CMC Biopharmaceuticals A/S   (Denmark)

Author keywords

Farnesol; Isoprenoids; Mevalonate pathway; Saccharomyces cerevisiae; Sesquiterpenes; Two phase fermentation

Indexed keywords

FARNESOL; ISOPRENOIDS; MEVALONATE PATHWAY; SESQUITERPENES; TWO PHASE FERMENTATION;

BIOSYNTHESIS; FERMENTATION; PLANTS (BOTANY); YEAST;

HYDROCARBONS;

CUBEBOL; FARNESYL DIPHOSPHATE; GALACTOSE; METHIONINE; PATCHOULOL; SESQUITERPENE; UNCLASSIFIED DRUG; VALENCENE; ERG9 PROTEIN, S CEREVISIAE; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; SQUALENE SYNTHASE;

ARTICLE; BIOSYNTHESIS; DOWN REGULATION; DRUG SYNTHESIS; DRUG TARGETING; ERG9 GENE; FERMENTATION; FUNGAL STRAIN; GENE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PROMOTER REGION; PROTEIN BLOOD LEVEL; QUANTITATIVE ANALYSIS; SOLID PHASE MICROEXTRACTION; SOLUBILITY; VOLATILIZATION; GENETICS; METABOLISM; METHODOLOGY; PLANT PHYSIOLOGY; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

DOWN-REGULATION; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; PLANT PHYSIOLOGY; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SESQUITERPENES;

EID: 38449112770     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21581     Document Type: Article

References (35)

1. Alper H, Miyaoku K, Stephanopoulos G. 2005. Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol 23:612-616.
2. Barkholt V, Jensen AL. 1989. Amino acid analysis: Determination of cysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive. Anal Biochem 177:318-322.
3. Breivik ON, Owades JL. 1957. Spectrophotometric semimicrodetermination of ergosterol in yeast. J Agric Food Chem 5:360-363.
4. Buurman ET, Blodgett AE, Hull KG, Carcanague D. 2004. Pyridines and pyrimidines mediating activity against an efflux-negative strain of Candida albicans through putative inhibition of lanosterol demethylase. Antimicrob Agents Chemother 48:313-318.
5. Cane DE. 1999. Sesquiterpene biosynthesis: Cyclization mechanisms. In: Barton D, Nakanishi K, Meth-Cohn O, editors. Comprehensive natural product chemistry, Vol. 2. Pergamon. Oxford: p. 155-200.
6. Carter OA, Peters RJ, Croteau R. 2003. Monoterpene biosynthesis pathway construction in Escherichia coli. Phytochem 64:425-433.
7. Chappell J. 1995. The biochemistry and molecular biology of isoprenoid metabolism. Plant Physiol 107:1-6.
8. Connolly JD, Hill RA. 1991. Dictionary of terpenoids. London: Chapman & Hall.
9. Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M. 2006. The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases. Arch Biochem Biophys 454:123-136.
10. DeJong JM, Liu Y, Bollon AP, Long RM, Jennewein S, Williams D, Croteau RB. 2006. Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae. Biotechnol Bioeng 93:212-224.
11. Dynesen J, Smits HP, Olsson L, Nielsen J. 1998. Carbon catabolite repression of invertase during batch cultivations of Saccharomyces cerevisiae. The role of glucose, fructose, and mannose. Appl Microbiol Biotechnol 50:579-582.
12. Erdeniz N, Mortensen UH, Rothstein R. 1997. Cloning-free PCR-based allele replacement methods. Genome Res 7:1174-1183.
13. Gardner RG, Hampton RY. 1999. A highly conserved signal controls degradation of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase in eukaryotes. J Biol Chem 274:31671-31678.
14. Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24:2519-2524.
15. Hornby JM, Kebaara BW, Nickerson KW. 2003. Farnesol biosynthesis in Candida albicans: Cellular response to sterol inhibition by zaragozic acid B. Antimicrob Agents Chemother 47:2366-2369.
16. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28.
17. Jackson BE, Hart-Wells EA, Matsuda SPT. 2003. Metabolic engineering to produce sesquiterpenes in yeast. Org Lett 5:1629-1632.
18. Kim S-W, Keasling JD. 2001. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng 72:408-415.
19. Lamacka M, Sajbidor J. 1997. Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods. Biotechnol Tech 11:723-725.
20. Martin VJJ, Yoshikuni Y, Keasling JD. 2001. The in vivo synthesis of plant sesquiterpenes by Escherichia coli. Biotechnol Bioeng 75:497-503.
21. Martin VJJ, Pitera DJ, Withers ST, Newman JD, Keasling JD. 2003. Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat Biotechnol 21:796-802.
22. Maury J, Asadollahi MA, Møller K, Clark A, Nielsen J. 2005. Microbial isoprenoid production: An example of green chemistry through metabolic engineering. Adv Biochem Eng Biotechnol 100:19-51.
23. McGarvey DJ, Croteau R. 1995. Terpenoid metabolism. Plant Cell 7:1015-1026.
24. Miura Y, Kondo K, Shimada H, Saito T, Nakamura K, Misawa N. 1998a. Production of lycopene by the food yeast, Candida utilis that does not naturally synthesize carotenoid. Biotechnol Bioeng 58:306-308.
25. Miura Y, Kondo K, Saito T, Shimada H, Fraser PD, Misawa N. 1998b. Production of the carotenoids lycopene, β-carotene, and astaxanthin in the food yeast Candida utilis. Appl Environ Microbiol 64:1226-1229.
26. Mountain HA, Byström AS, Larsen JT, Korch C. 1991. Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae. Yeast 7:781-803.
27. Newman JD, Marshall J, Chang M, Nowroozi F, Paradise E, Pitera D, Newman KL, Keasling JD. 2006. High-level production of amorpha-4, 11-diene in a two-phase partitioning bioreactor of metabolically engineered Escherichia coli. Biotechnol Bioeng 95:684-691.
28. Pitera DJ, Paddon CJ, Newman JD, Keasling JD. 2007. Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli. Metab Eng 9:193-207.
29. Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MC, Withers ST, Shiba Y, Sarpong R, Keasling JD. 2006. Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 440:940-943.
30. Shimada H, Kondo K, Fraser PD, Miura Y, Saito T, Misawa N. 1998. Increased carotenoid production by the food yeast Candida utilis through metabolic engineering of the isoprenoid pathway. Appl Environ Microbiol 64:2676-2680.
31. Song L. 2003. Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants. Anal Biochem 317:180-185.
32. Verduyn C, Postma E, Scheffers WA, van Dijken JP. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8:501-517.
33. Wanke M, Skorupinska-Tudek K, Swiezewska E. 2001. Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. Acta Biochim Pol 48:663-672.
34. Withers ST, Keasling JD. 2007. Biosynthesis and engineering of isoprenoid small molecules. Appl Microbiol Biotechnol 73:980-990.
35. Yamano S, Ishii T, Nakagawa M, Ikenaga H, Misawa N. 1994. Metabolic engineering for production of β-carotene and lycopene in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 58:1112-1114.