Coexpression and secretion of endoglucanase and phytase genes in Lactobacillus reuteri

International Journal of Molecular Sciences

Volumn 15, Issue 7, 2014, Pages 12842-12860

  Wang, Lei ^a   Yang, Yuxin ^a   Cai, Bei ^a   Cao, Pinghua ^a   Yang, Mingming ^a   Chen, Yulin ^a  

^a NORTHWEST A F UNIVERSITY   (China)

Author keywords

Broiler; Coexpression; Lactobacillus reuteri; Phytase; Secretion; endoglucanase

Indexed keywords

BACTERIAL ENZYME; CALCIUM; CARBOXYMETHYLCELLULOSE; GLUCAN SYNTHASE; LACTATE DEHYDROGENASE; PHOSPHORUS; PHYTASE; BACTERIAL PROTEIN; CELLULASE; FUNGAL PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASPERGILLUS FUMIGATUS; BACILLUS SUBTILIS; BACTERIAL GENE; BACTEROIDES VULGATUS; BIFIDOBACTERIUM; BROILER; CECUM; CONTROLLED STUDY; DIET SUPPLEMENTATION; ENZYME ACTIVITY; ENZYME RELEASE; ESCHERICHIA COLI; FUNGAL GENE; GENE EXPRESSION; GENETIC TRANSFORMATION; GROWTH ACCELERATION; LACTOBACILLUS CASEI; LACTOBACILLUS REUTERI; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; TIBIA; VEILLONELLA; ANIMAL; BODY WEIGHT; CHICKEN; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIOLOGY; PHYSIOLOGY;

6-PHYTASE; ANIMALS; ASPERGILLUS FUMIGATUS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BODY WEIGHT; CECUM; CELLULASE; CHICKENS; FUNGAL PROTEINS; LACTOBACILLUS REUTERI;

EID: 84904678280     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms150712842     Document Type: Article

References (60)

1. Yin, Q.Q.; Zheng, Q.H.; Kang, X.T. Biochemical characteristics of phytases from fungi and the transformed microorganism. Anim. Feed Sci. Technol. 2007, 132, 341-350.
2. Liu, J.R.; Yu, B.; Liu, F.H.; Cheng, K.J.; Zhao, X. Expression of rumen microbial fibrolytic enzyme genes in probioticLactobacillus reuteri. Appl. Environ. Microbiol. 2005, 71, 6769-6775.
3. Selle, P.H.; Ravindran, V.; Caldwell, R.A.; Bryden, W.L. Phytate and phytase: Consequences for protein utilisation. Nutr. Res. Rev. 2000, 13, 255-278.
4. Choct, M.; Annison, G. Anti-nutritive effect of wheat pentosans in broiler chickens: Roles of viscosity and gut microflora. Br. Poult. Sci. 1992, 33, 821-834.
5. Bedford, M.R.; Schulze, H. Exogenous enzymes for pigs and poultry. Nutr. Res. Rev. 1998, 11, 91-114.
6. Correll, D.L. Phosphorus: A rate limiting nutrient in surface waters. Poult. Sci. 1999, 78, 674-682.
7. Choi, Y.M.; Suh, H.J.; Kim, J.M. Purification and properties of extracellular phytase from Bacillus sp. KHU-10. J. Protein Chem. 2001, 20, 287-292.
8. Hall, J.; Ali, S.; Surani, M.A.; Hazlewood, G.P.; Clark, A.J.; Simons, J.P.; Hirst, B.H.; Gilbert, H.J. Manipulation of the repertoire of digestive enzymes secreted into the gastrointestinal tract of transgenic mice. Nat. Biotechnol. 1993, 11, 376-379.
9. Knowlton, K.F.; Parsons, C.M.; Cobb, C.W.; Wilson, K.F. Exogenous phytase plus cellulase and phosphorus excretion in lactating dairy cows. Prof. Anim. Sci. 2005, 21, 212-216.
10. Waldroup, P.W.; Kersey, J.H.; Saleh, E.A.; Fritts, C.A.; Yan, F.; Stilborn, H.L.; Crum, R.C., Jr.; Raboy, V. Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn with and without microbial phytase. Poult. Sci. 2000, 79, 1451-1459.
11. Cromwell, G.L.; Stahly, T.S.; Coffey, R.D.; Monegue, H.J.; Randolph, J.H. Efficacy of phytase in improving the bioavailability of phosphorus in soybeanmeal and corn-soybean meal diets for pigs. J. Anim. Sci. 1993, 71, 1831-1840.
12. Simons, P.C.M.; Versteegh, H.A.J.; Jongbloed, A.W.; Kemme, P.A.; Slump, P.; Bos, K.D.; Wolters, M.G.E.; Beudeker, R.F.; Verschoor, G.J. Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br. J. Nutr. 1990, 64, 525-540.
13. Mathlouthi, N.; Juin, H.; Larbier, M. Effect of xylanase and β-glucanase supplementation of wheat-or wheat-and barley-based diets on the performance of male turkeys. Br. Poult. Sci. 2003, 44, 291-298.
14. Mathlouthi, N.; Mohamed, M.A.; Larbier, M. Effect of enzyme preparation containing xylanase and β-glucanase on performance of laying hens fed wheat/barley-ormaize/soybean meal-based diets. Br. Poult. Sci. 2003, 44, 60-66.
15. Bates, E.E.; Gilbert, H.J.; Hazlewood, G.P.; Huckle, J.; Laurie, J.I.; Mann, S.P. Expression of a Clostridium thermocellumendoglucanase gene in Lactobacillus plantarum. Appl. Environ. Microbiol. 1989, 55, 2095-2097.
16. Liu, J.R.; Yu, B.; Zhao, X.; Cheng, K.J. Coexpression ofrumen microbial β-glucanase and xylanase genes in Lactobacillus reuteri. Appl. Microbiol. Biotechnol. 2007, 77, 117-124.
17. Cho, J.S.; Choi, Y.J.; Chung, D.K. Expression of Clostridium thermocellumendoglucanase gene in Lactobacillus gasseriand Lactobacillus johnsoniiand characterization of the genetically modified probiotic lactobacilli. Curr. Microbiol. 2000, 40, 257-263.
18. Zegers, N.D.; Kluter, E.; van der Stap, H.; van Dura, E.; van Dalen, P.; Shaw, M.; Baillie, L. Expression of the protective antigen of Bacillus anthracisby Lactobacillus casei: Towards the development of an oral vaccine against anthrax. J. Appl. Microbiol. 2001, 87, 309-314.
19. Bateup, J.M.; McConnell, M.A.; Jenkinson, H.F.; Tannock, G.W. Comparison of Lactobacillus strains with respect to bile salt hydrolase activity, colonization of the gastrointestinal tract, and growth rate of the murine host. Appl. Environ. Microbiol. 1995, 61, 1147-1149.
20. Zuo, R.; Chang, J.; Yin, Q.; Chen, L.; Chen, Q.; Yang, X.; Zheng, Q.; Ren, G.; Feng, H. Phytase gene expression in Lactobacillusand analysis of its biochemical characteristics. Microbiol. Res. 2010, 165, 329-335.
21. Kerovuo, J.; Tynkkynen, S. Expression of Bacillus subtilisphytase inLactobacillus plantarum 755. Lett. Appl. Microbiol. 2001, 30, 325-329.
22. Pérez-Arellano, I.; Pérez-Martínez, G. Optimization of the green fluorescent protein (GFP) expression from a lactose-inducible promoter in Lactobacillus casei. FEMS Microbiol. Lett. 2006, 222, 123-127.
23. Yu, Q.H.; Dong, S.M.; Zhu, W.Y.; Yang, Q. Use of green fluorescent protein to monitor Lactobacillusin the gastro-intestinal tract of chicken. FEMS Microbiol. Lett. 2007, 275, 207-213.
24. Wells, J.M.; Mercenier, A. Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria. Nat. Rev. Microbiol. 2008, 6, 349-362.
25. Rodri{dotless}guez, J.M.; Marti{dotless}nez, M.I.; Horn, N.; Dodd, H.M. Heterologous production of bacteriocins by lactic acid bacteria. Int. J. Food Microbiol. 2003, 80, 101-116.
26. Liu, J.R.; Lai, S.F.; Yu, B. Evaluation of an intestinal Lactobacillus reuteristrain expressing rumen fungal xylanase as a probiotic for broiler chickens fed on a wheat-based diet. Br. Poult. Sci. 2007, 48, 507-514.
27. Sieo, C.C.; Abdullah, N.; Tan, W.S.; Ho, Y.W. In vivostudy on the persistence of transformed β-glucanase-producing Lactobacillusstrains in the gastrointestinal tract of chickens. J. Anim. Feed Sci. 2006, 15, 261.
28. Stephenson, D.P.; Moore, R.J.; Allison, G.E. Transformation of, and heterologous protein expression in, Lactobacillus agilisand Lactobacillus vaginalisisolates from the chicken gastrointestinal tract. Appl. Environ. Microbiol. 2011, 77, 220-228.
29. Gory, L.; Montel, M.C.; Zagorec, M. Use of green fluorescent protein to monitor Lactobacillus sakei in fermented meat products. FEMS Microbiol. Lett. 2006, 194, 127-133.
30. Boot, H.J.; Kolen, C.P.; Andreadaki, F.J.; Leer, R.J.; Pouwels, P.H. The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA. J. Bacteriol. 1996, 178, 5388-5394.
31. Djordjevic, G.; Bojovic, B.; Banina, A.; Topisirovic, L. Cloning of promoter-like sequences from Lactobacillus paracaseisubsp. paracaseiCG11 and their expression in Escherichia coli, Lactococcus lactis, andLactobacillus reuteri. Can. J. Microbiol. 1994, 40, 1043-1050.
32. Chang, T.L.Y.; Chang, C.H.; Simpson, D.A.; Xu, Q.; Martin, P.K.; Lagenaur, L.A.; Schoolnik, G.K.; Ho, D.D.; Hillier, S.L.; Holodniy, M. Inhibition ofHIV infectivity by a natural human isolate of Lactobacillusjensenii engineered to express functional two-domain CD4. Proc. Natl. Acad. Sci. USA 2003, 100, 11672-11677.
33. Le Loir, Y.; Nouaille, S.; Commissaire, J.; Bretigny, L.; Gruss, A.; Langella, P. Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis. Appl. Environ. Microbiol. 2001, 67, 4119-4127.
34. Asseldonk, M.; Vos, W.M.; Simons, G. Functional analysis of the Lactococcus lactisusp45 secretion signal in the secretion of a homologous proteinase and a heterologous α-amylase. Mol. Gen. Genet. 1993, 240, 428-434.
35. Steidler, L.; Hans, W.; Schotte, L.; Neirynck, S.; Obermeier, F.; Falk, W.; Fiers, W.; Remaut, E. Treatment of murine colitis by Lactococcus lactissecreting interleukin-10. Science 2000, 289, 1352-1355.
36. Mercenier, A.; Pouwels, P.H.; Chassy, B.M. Genetics and Biotechnology of Lactic Acid Bacteria; Springer Netherlands: Blackie Academic and Professional, Glasgow, UK, 1994; pp. 252-293.
37. Hols, P.; Ferain, T.; Garmyn, D.; Bernard, N.; Delcour, J. Use of homologous expression-secretion signals and vector-free stable chromosomal integration inengineering of Lactobacillus plantarum for alpha-amylase and levanase expression. Appl. Environ. Microbiol. 1994, 60, 1401-1413.
38. Savijoki, K.; Kahala, M.; Palva, A. High level heterologous protein production in Lactococcus and Lactobacillususing a new secretion system based on the Lactobacillus brevisS-layer signals. Gene 1997, 186, 255-262.
39. Li, W.; Yang, M.M.; Zhang, G.Q.; He, W.L.; Li, Y.X.; Chen, Y.L. Electrotransformation and expression of Cellulase genes in wild-type Lactobacillus reuteri. J. Mol. Microbiol. Biotechnol. 2012, 22, 228-234.
40. Heng, N.C.; Jenkinson, H.F.; Tannock, G.W. Cloning and expression of an endo-1, 3-1, 4-β-glucanase gene from Bacillus maceransin Lactobacillus reuteri. Appl. Environ. Microbiol. 1997, 63, 3336-3340.
41. Selle, P.H.; Ravindran, V. Microbial phytase in poultry nutrition. Anim. Feed Sci. Technol. 2007, 135, 1-41.
42. Slominski, B.A. Recent advances in research on enzymes for poultry diets. Poult. Sci. 2011, 90, 2013-2023.
43. Yu, B.; Liu, J.R.; Hsiao, F.S.; Chiou, P.W.S. Evaluation of Lactobacillus reuteriPg4 strain expressing heterologous β-glucanase as a probiotic in poultry diets based on barley. Anim. Feed Sci. Technol. 2008, 141, 82-91.
44. Jin, L.Z.; Ho, Y.W.; Abdullah, N.; Jalaludin, S. Growth performance, intestinal microbial populations, and serum cholesterol ofbroilers fed diets containing Lactobacilluscultures. Poult. Sci. 1998, 77, 1259-1265.
45. Askelson, T.E.; Campasino, A.; Lee, J.T.; Duong, T. Evaluation of phytate-degrading Lactobacillus culture administration to broiler chickens. Appl. Environ. Microbiol. 2014, 80, 943-950.
46. Watkins, B.A.; Miller, B.F. Competitive gut exclusion of avian pathogens by Lactobacillus acidophilusin gnotobiotic chicks. Poult. Sci. 1983, 62, 1772-1779.
47. Perney, K.M.; Cantor, A.H.; Straw, M.L.; Herkelman, K.L. The effect of dietary phytase on growth performance and phosphorus utilization of broiler chicks. Poult. Sci. 1993, 72, 2106-2114.
48. Leeson, S.; Namkung, H.; Cottrill, M.; Forsberg, C.W. Efficacy of new bacterial phytase in poultry diets. Can. J. Anim. Sci. 2000, 80, 527-528.
49. Ahmad, T.; Rasool, S.; Sarwar, M.; Haq, A.; Hasan, Z. Effect of microbial phytase produced from a fungus Aspergillus nigeron bioavailability of phosphorus and calcium in broiler chickens. Anim. Feed Sci. Technol. 2000, 83, 103-114.
50. Sebastian, S.; Touchburn, S.P.; Chavez, E.R. Implications of phytic acid and supplemental microbial phytase in poultry nutrition: A review. Worlds Poult. Sci. J. 1998, 54, 27-47.
51. Gong, J.; Si, W.; Forster, R.J.; Huang, R.; Yu, H.; Yin, Y.; Yang, C.; Han, Y. 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: From crops to ceca. FEMS Microbiol. Ecol. 2007, 59, 147-157.
52. Bazzocchi, G.; Gionchetti, P.; Almerigi, P.F.; Amadini, C.; Campieri, M. Intestinal microflora and oral bacteriotherapy in irritable bowel syndrome. Dig. Liver Dis. 2002, 34, S48-S53.
53. Mathlouthi, N.; Mallet, S.; Saulnier, L.; Quemener, B.; Larbier, M. Effects of xylanase and β-glucanase addition on performance, nutrient digestibility, and physico-chemical conditions in the small intestine contents and caecal microflora of broiler chickens fed a wheat and barley-based diet. Anim. Res. 2002, 51, 395-406.
54. Djouzi, Z.; Andlueux, C. Compared effects of three oligosaccharides on metabolism of intestinal microflora in rats inoculatedwith a human faecal flora. Br. J. Nutr. 1997, 78, 313-324.
55. Sambrook, J.; Russell, D.W. Molecular Cloning: A Laboratory Manual, 3nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, NY, USA, 2001.
56. Serror, P.; Sasaki, T.; Ehrlich, S.D.; Maguin, E. Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricusand L. delbrueckii subsp. lactiswith various plasmids. Appl. Environ. Microbiol. 2002, 68, 46-52.
57. Walker, D.C.; Klaenhammer, T.R. Isolation of a novel IS3 group insertion element and construction of an integration vector for Lactobacillusspp. J. Bacteriol. 1994, 176, 5330-5340.
58. Teather, R.M.; Wood, P.J. Use of Congo red-polysaccharide interactionsin enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 1982, 43, 777-780.
59. Piard, J.C.; Hautefort, I.; Fischetti, V.A.; Ehrlich, S.D.; Fons, M.; Gruss, A. Cell wall anchoring of the Streptococcus pyogenesM6 protein in various lactic acid bacteria. J. Bacteriol. 1997, 179, 3068-3072.
60. Mappley, L.J.; Tchórzewska, M.A.; Nunez, A.; Woodward, M.J.; Bramley, P.M.; La Ragione, R.M. Oral treatment of chickens with Lactobacillus reuteriLM1 reduces Brachyspira pilosicoli-induced pathology. J. Med. Microbiol. 2013, 62, 287-296.