Electrotransformation and expression of cellulase genes in wild-type lactobacillus reuteri

Journal of Molecular Microbiology and Biotechnology

Volumn 22, Issue 4, 2012, Pages 228-234

  Li, Wang ^a,b   Yang, Ming Ming ^a   Zhang, Guang Qin ^b   He, Wan Ling ^b   Li, Yuan Xiao ^b   Chen, Yu Lin ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Cellulase genes; Expression; Lactobacillus reuteri; Transformation

Indexed keywords

BACTERIAL DNA; BACTERIAL VECTOR; CELLULASE; MESSENGER RNA;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENOME; BACTERIAL STRAIN; BACTERIUM CULTURE; ELECTROTRANSFORMATION; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE EXPRESSION; LACTOBACILLUS REUTERI; MOLECULAR CLONING; NONHUMAN; PLASMID; REAL TIME POLYMERASE CHAIN REACTION; WILD TYPE;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; ELECTROPORATION; ENZYME ACTIVATION; ENZYME ASSAYS; ESCHERICHIA COLI; GENES, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; LACTOBACILLUS REUTERI; PLASMIDS; RNA, BACTERIAL; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; LACTOBACILLUS REUTERI;

EID: 84865853416     PISSN: 14641801     EISSN: 16602412     Source Type: Journal    
DOI: 10.1159/000341906     Document Type: Article

References (27)

1. Bayer EA, Chanzy H, Lamed R, Shoham Y: Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol 1998; 8: 548-557. (Pubitemid 28486379)
2. Chaurasia AK, Parasnis A, Apte SK: An integrative expression vector for strain improvement and environmental applications of the nitrogen fixing cyanobacterium, Anabaena sp. strain pcc7120. J Microbiol Methods 2008; 73: 133-141.
3. Ferenczi S, Ganyu A, Blaha B, Semsey S, Nagy T, Csiszovszki Z, Orosz L, Papp PP: Integrative plasmid vector for constructing single-copy reporter systems to study gene regulation in Rhizobium meliloti and related species. Plasmid 2004; 52: 57-62. (Pubitemid 38813161)
4. Graminha EBN, Goncalves AZL, Pirota RDPB, Balsalobre MAA, Da Silva R, Gomes E: Enzyme production by solid-state fermentation: application to animal nutrition. Anim Feed Sci Technol 2008; 144: 1-22. (Pubitemid 351783342)
5. Heng NC, Jenkinson HF, Tannock GW: Cloning and expression of an endo-1,3-1,4-glucanase gene from Bacillus macerans in Lactobacillus reuteri . Appl Environ Microbiol 1997; 63: 3336-3340. (Pubitemid 27337842)
6. Hernandez M, Esteve T, Prat S, Pla M: Development of real-time PCR systems based on SYBR green I, Amplifluor TM and TaqMan technologies for specific quantitative detection of the transgenic maize event GA21. J Cereal Sci 2004; 39: 99-107. (Pubitemid 38098076)
7. Hormann S, Vogel RF, Ehrmann M: Construction of a new reporter system to study the NaCl-dependent dnaK promoter activity of Lactobacillus sanfranciscensis . Appl Microbiol Biotechnol 2006; 70: 690-697. (Pubitemid 43760505)
8. Hubalek Z: Protectants used in the cryopreservation of microorganisms. Cryobiology 2003; 46: 205-229. (Pubitemid 36724563)
9. Inoue T, Moriya S, Ohkuma M, Kudo T: Molecular cloning and characterization of a cellulase gene from a symbiotic protist of the lower termite, Coptotermes formosanus . Gene 2005; 349: 67-75.
10. Lee Y-J, Kim B-K, Lee B-H, Jo K-I, Lee N-K, Chung C-H, Lee Y-C, Lee J-W: Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour Technol 2008; 99: 378-386. (Pubitemid 350011138)
11. Li W, Huan X, Zhou Y, Ma Q, Chen Y: Simultaneous cloning and expression of two cellulase genes from Bacillus subtilis newly isolated from golden takin ( Budorcas taxicolor bedfordi ). Biochem Biophys Res Commun 2009; 383: 397-400.
12. Li W, Zhang W-W, Yang M-M, Chen Y-L: Cloning of the thermostable cellulase gene from newly isolated Bacillus subtilis and its expression in Escherichia coli . Mol Biotechnol 2008; 40: 195-201.
13. Liu JR, Yu B, Lin SH, Cheng KJ, Chen YC: Direct cloning of a xylanase gene from the mixed genomic DNA of rumen fungi and its expression in intestinal Lactobacillus reuteri . FEMS Microbiol Lett 2005a;251: 233-241. (Pubitemid 41383874)
14. Liu JR, Yu B, Liu FH, Cheng KJ, Zhao X: Expression of rumen microbial fibrolytic enzyme genes in probiotic Lactobacillus reuteri . Appl Environ Microbiol 2005b;71: 6769-6775. (Pubitemid 43194163)
15. Liu JR, Yu B, Zhao X, Cheng KJ: Coexpression of rumen microbial-glucanase and xylanase genes in Lactobacillus reuteri . Appl Microbiol Biotechnol 2007; 77: 117-124. (Pubitemid 47573304)
16. Mason CK, Collins MA, Thompson K: Modified electroporation protocol for lactobacilli isolated from the chicken crop facilitates transformation and the use of a genetic tool. J Microbiol Methods 2005; 60: 353-363. (Pubitemid 40093061)
17. Miller GL: Use of dinitrosalicylic as reagent for the determination of reducing sugars. Anal Chem 1959; 31: 426-428.
18. Morita H, Toh H, Fukuda S, Horikawa H, Oshima K, Suzuki T, Murakami M, Hisamatsu S, Kato Y, Takizawa T, Fukuoka H, Yoshimura T, Itoh K, O'Sullivan DJ, McKay LL, Ohno H, Kikuchi J, Masaoka T, Hattori M: Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production. DNA Res 2008; 15: 151-161.
19. Naumenko VS, Osipova DV, Kostina EV, Kulikov AV: Utilization of a two-standard system in real-time PCR for quantification of gene expression in the brain. J Neurosci Methods 2008; 170: 197-203.
20. O'Connor-Robison CI, Nielsen BD, Morris R: Cellulase supplementation does not improve the digestibility of a high-forage diet in horses. J Equine Vet Sci 2007; 27: 535-538. (Pubitemid 350216345)
21. Rizzatti AC, Jorge JA, Terenzi HF, Rechia CG, Polizeli ML: Purification and properties of a thermostable extracellular-D-xylosidase produced by a thermotolerant Aspergillus phoenicis . J Ind Microbiol Biotechnol 2001; 26: 156-160. (Pubitemid 32336865)
22. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2. New York, Cold Spring Harbor Laboratory Press, 1989.
23. Schwab C, Walter J, Tannock GW, Vogel RF, Ganzle MG: Sucrose utilization and impact of sucrose on glycosyltransferase expression in Lactobacillus reuteri . Syst Appl Microbiol 2007; 30: 433-443. (Pubitemid 47126764)
24. Serror P, Sasaki T, Ehrlich SD, Maguin E: Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and l. Delbrueckii subsp. lactis with various plasmids. Appl Environ Microbiol 2002; 68: 46-52. (Pubitemid 34031161)
25. Teresa Alegre M, Carmen Rodriguez M, Mesas JM: Transformation of Lactobacillus plantarum by electroporation with in vitro modified plasmid DNA. FEMS Microbiol Lett 2004; 241: 73-77. (Pubitemid 39531907)
26. Yang C-Y, Ho Y-C, Pang J-C, Huang S-S, Tschen JS-M: Cloning and expression of an antifungal chitinase gene of a novel Bacillus subtilis isolate from Taiwan potato field. Bioresour Technol 2009; 100: 1454-1458.
27. Zhao F-Y, Lin J-F, Zeng X-L, Guo L-Q, Wang Y-H, You L-R: Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea . Bioresour Technol 2010; 101: 6482-6486.