Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy ABC Highlights: Authored by Rising Stars and Top Experts

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 20, 2014, Pages 4797-4813

  Macháň, Radek ^a   Kapusta, Peter ^b   Hof, Martin ^b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b J HEYROVSKÝ INSTITUTE OF PHYSICAL CHEMISTRY   (Czech Republic)

Author keywords

Filtered fluorescence correlation spectroscopy; Fluorescence lifetime correlation spectroscopy; Fluorescence spectral correlation spectroscopy; Raster lifetime image correlation spectroscopy; Time correlated single photon counting

Indexed keywords

FLUORESCENCE; FLUORESCENCE SPECTROSCOPY;

CORRELATION SPECTROSCOPY; FLUORESCENCE CORRELATION SPECTROSCOPY; IMAGE CORRELATION SPECTROSCOPY; SPECTRAL CORRELATION; TIME-CORRELATED SINGLE PHOTON COUNTING;

FLUORESCENCE MICROSCOPY;

DATA INTERPRETATION, STATISTICAL; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INSTRUMENTATION; METHODS; MICROSCOPY, FLUORESCENCE; MODELS, STATISTICAL; SPECTROMETRY, FLUORESCENCE;

DATA INTERPRETATION, STATISTICAL; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; MODELS, STATISTICAL; SPECTROMETRY, FLUORESCENCE;

EID: 84904468860     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-7892-7     Document Type: Review

References (108)

1. Rigler R, Mets U, Widengren J, Kask P (1993) Fluorescence correlation spectroscopy with high count rate and low background - analysis of translational diffusion. Eur Biophys J Biophys Lett 22(3):169-175. doi: 10.1007/BF00185777
2. Ries J, Schwille P (2012) Fluorescence correlation spectroscopy. BioEssays 34(5):361-368. doi: 10.1002/bies.201100111
3. Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E (2005) Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophys J 88(5):L33-36. doi: 10.1529/biophysj.105.061788
4. Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E (2005) Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys J 89(2):1317-1327. doi: 10.1529/biophysj.105.062836
5. Borst JW, Visser AJWG (2010) Fluorescence lifetime imaging microscopy in life sciences. Meas Sci Technol 21(10):102002. doi: 10.1088/0957-0233/21/10/ 102002
6. Becker W (2012) Fluorescence lifetime imaging - techniques and applications. J Microsc 247(2):119-136. doi: 10.1111/j.1365-2818.2012.03618.x
7. Lakowicz JR, Szmacinski H, Nowaczyk K, Berndt KW, Johnson M (1992) Fluorescence lifetime imaging. Anal Biochem 202(2):316-330. doi: 10.1016/0003-2697(92)90112-K
8. Pietraszewska-Bogiel A, Gadella TWJ (2011) FRET microscopy: from principle to routine technology in cell biology. J Microsc 241(2):111-118. doi: 10.1111/j.1365-2818.2010.03437.x
9. Day RN, Periasamy A, Schaufele F (2001) Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus. Methods 25(1):4-18. doi: 10.1006/meth.2001.1211
10. Suhling K, Siegel J, Lanigan PMP, Leveque-Fort S, Webb SED, Phillips D, Davis DM, French PMW (2004) Time-resolved fluorescence anisotropy imaging applied to live cells. Opt Lett 29(6):584-586. doi: 10.1364/ol.29.000584
11. Dix JA, Verkman AS (1990) Mapping of fluorescence anisotropy in living cells by ratio imaging - application to cytoplasmic viscosity. Biophys J 57(2):231-240. doi: 10.1016/S0006-3495(90)82526-1
12. Clayton AHA, Hanley QS, Arndt-Jovin DJ, Subramaniam V, Jovin TM (2002) Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM). Biophys J 83(3):1631-1649. doi: 10.1016/S0006-3495(02)73932-5
13. Bolte S, Cordelieres FP (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc-Oxf 224:213-232. doi: 10.1111/j.1365-2818.2006.01706.x
14. Zinchuk V, Zinchuk O, Okada T (2007) Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: Pushing pixels to explore biological phenomena. Acta Histochem Cytochem 40(4):101-111. doi: 10.1267/ahc.07002
15. Schwille P, Meyer-Almes FJ, Rigler R (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72(4):1878-1886. doi: 10.1016/S0006-3495(97)78833-7
16. Ries J, Petrášek Z, García-Sáez AJ, Schwille P (2010) A comprehensive framework for fluorescence cross-correlation spectroscopy. New J Phys 12(11):113009. doi: 10.1088/1367-2630/12/11/113009
17. Hwang LC, Wohland T (2007) Recent advances in fluorescence cross-correlation spectroscopy. Cell Biochem Biophys 49(1):1-13. doi: 10.1007/s12013-007-0042-5
18. Foo YH, Naredi-Rainer N, Lamb DC, Ahmed S, Wohland T (2012) Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy. Biophys J 102(5):1174-1183. doi: 10.1016/j.bpj.2012.01.040
19. Böhmer M, Wahl M, Rahn HJ, Erdmann R, Enderlein J (2002) Time-resolved fluorescence correlation spectroscopy. Chem Phys Lett 353(5-6):439-445. doi: 10.1016/s0009-2614(02)00044-1
20. Gregor I, Enderlein J (2007) Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy. Photochem Photobiol Sci 6(1):13-18. doi: 10.1039/b610310c
21. Kapusta P, Macháň R, Benda A, Hof M (2012) Fluorescence Lifetime Correlation Spectroscopy (FLCS): concepts, applications and outlook. Int J Mol Sci 13(10):12890-12910. doi: 10.3390/ijms131012890
22. Benda A, Kapusta P, Hof M, Gaus K (2014) Fluorescence spectral correlation spectroscopy (FSCS) for probes with highly overlapping emission spectra. Opt Express 22(3):2973-2988. doi: 10.1364/oe.22.002973
23. Felekyan S, Kalinin S, Sanabria H, Valeri A, Seidel CA (2012) Filtered FCS: species auto- and cross-correlation functions highlight binding and dynamics in biomolecules. ChemPhysChem 13(4):1036-1053. doi: 10.1002/cphc.201100897
24. Felekyan S, Sanabria H, Kalinin S, Kuhnemuth R, Seidel CAM (2013) Analyzing forster resonance energy transfer with fluctuation algorithms. In: Tetin SY (ed) Fluorescence fluctuation spectroscopy, vol 519. Methods in enzymology. Elsevier Academic Press Inc, San Diego, pp 39-85. doi: 10.1016/b978-0-12-405539-1.00002-6
25. Felekyan S, Kalinin S, Valeri A, Seidel CAM (2009) Filtered FCS and species cross correlation function. In: Periasamy A, So PTC (eds) Multiphoton Microscopy in the Biomedical Sciences IX, vol 7183. Proceedings of SPIE. Spie-Int Soc Optical Engineering, Bellingham, p 71830D. doi: 10.1117/12.814876
26. Müller BK, Zaychikov E, Brauchle C, Lamb DC (2005) Pulsed interleaved excitation. Biophys J 89(5):3508-3522. doi: 10.1529/biophysj.105. 064766
27. Lee NK, Kapanidis AN, Wang Y, Michalet X, Mukhopadhyay J, Ebright RH, Weiss S (2005) Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation. Biophys J 88(4):2939-2953. doi: 10.1529/biophysj.104.054114
28. Chen JJ, Irudayaraj J (2010) Fluorescence lifetime cross correlation spectroscopy resolves EGFR and antagonist interaction in live cells. Anal Chem 82(15):6415-6421. doi: 10.1021/ac101236t
29. Boswell C, Utzinger U (2011) Spectra Database hosted at the University of Arizona. University of Arizona. http://www.spectra.arizona.edu/. Accessed 3 Jan 2014
30. Kapusta P, Wahl M, Benda A, Hof M, Enderlein J (2007) Fluorescence lifetime correlation spectroscopy. J Fluoresc 17(1):43-48. doi: 10.1007/s10895-006-0145-1
31. Humpolíčková J, Beranová L, Štěpánek M, Benda A, Procházka K, Hof M (2008) Fluorescence lifetime correlation spectroscopy reveals compaction mechanism of 10 and 49 kbp DNA and differences between polycation and cationic surfactant. J Phys Chem B 112(51):16823-16829. doi: 10.1021/jp806358w
32. Hendrix J, Schrimpf W, Holler M, Lamb DC (2013) Pulsed interleaved excitation fluctuation imaging. Biophys J 105(4):848-861. doi: 10.1016/j.bpj.2013.05.059
33. Humpolíčková J, Sýkora J, Kapusta P, Wahl M, Benda A, Enderlein J, Hof M (2007) Fluorescence lifetime correlation spectroscopy. Proc Soc Photo Opt Instrum Eng 6583:658308. doi: 10.1117/12.736637
34. Ortmann U, Wahl M, Kapusta P (2008) Time-resolved fluorescence: novel technical solutions. In: Resch-Genger U (ed) Standardization and quality assurance in fluorescence measurements I, vol 5. Springer series on fluorescence. Springer, Berlin Heidelberg, pp 259-275. doi: 10.1007/4243-2008- 048
35. Benda A, Hof M, Wahl M, Patting M, Erdmann R, Kapusta P (2005) TCSPC upgrade of a confocal FCS microscope. Rev Sci Instrum 76(3):033106. doi: 10.1063/1.1866814
36. Mütze J, Ohrt T, Schwille P (2011) Fluorescence correlation spectroscopy in vivo. Laser Photon Rev 5(1):52-67. doi: 10.1002/lpor.200910041
37. Patting M, Wahl M, Kapusta P, Erdmann R (2007) Dead-time effects in TCSPC data analysis. In: Prochazka I, Migdall AL, Pauchard A, Dusek M, Hillery MS, Schleich WP (eds) Photon Counting Applications, Quantum Optics, and Quantum Cryptography, vol 6583. Proceedings of SPIE. Spie-Int Soc Optical Engineering, Bellingham, p 658307. doi: 10.1117/12.722804
38. Otosu T, Ishii K, Tahara T (2013) Note: simple calibration of the counting-rate dependence of the timing shift of single photon avalanche diodes by photon interval analysis. Rev Sci Instrum 84(3):036105. doi: 10.1063/1.4794769
39. Paredes JM, Casares S, Ruedas-Rama MJ, Fernandez E, Castello F, Varela L, Orte A (2012) Early amyloidogenic oligomerization studied through fluorescence lifetime correlation spectroscopy. Int J Mol Sci 13(8):9400-9418. doi: 10.3390/ijms13089400
40. Padilla-Parra S, Auduge N, Coppey-Moisan M, Tramier M (2011) Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell. Microsc Res Tech 74(8):788-793. doi: 10.1002/jemt.21015
41. Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M (2014) Quantitative Study of Protein-Protein Interactions in Live Cell by Dual-Color Fluorescence Correlation Spectroscopy. In: Engelborghs Y, Visser AJWG (eds) Fluorescence Spectroscopy and Microscopy, vol 1076. Methods in Molecular Biology. Humana Press, pp 683-698. doi: 10.1007/978-1-62703-649-8-31
42. Paredes JM, Crovetto L, Orte A, Alvarez-Pez JM, Talavera EM (2011) Influence of the solvent on the ground- and excited-state buffer-mediated proton-transfer reactions of a xanthenic dye. Phys Chem Chem Phys 13(4):1685-1694. doi: 10.1039/c0cp01232g
43. Paredes JM, Crovetto L, Orte A, Lopez SG, Talavera EM, Alvarez-Pez JM (2011) Photophysics of the interaction between a fluorescein derivative and Ficoll. J Phys Chem A 115(46):13242-13250. doi: 10.1021/jp204666j
44. Paredes JM, Garzon A, Crovetto L, Orte A, Lopez SG, Alvarez-Pez JM (2012) Effects of the anion salt nature on the rate constants of the aqueous proton exchange reactions. Phys Chem Chem Phys 14(16):5795-5800. doi: 10.1039/c2cp24058k
45. Orte A, Ruedas-Rama MJ, Paredes JM, Crovetto L, Alvarez-Pez JM (2011) Dynamics of water-in-oil nanoemulsions revealed by fluorescence lifetime correlation spectroscopy. Langmuir 27(21):12792-12799. doi: 10.1021/la202004d
46. Humpolíčková J, Benda A, Sýkora J, Macháň R, Kral T, Gasinska B, Enderlein J, Hof M (2008) Equilibrium dynamics of spermine-induced plasmid DNA condensation revealed by fluorescence lifetime correlation spectroscopy. Biophys J 94(3):L17-L19. doi: 10.1529/biophysj.107.122408
47. Humpolíčková J, Štěpánek M, Kral T, Benda A, Procházka K, Hof M (2008) On mechanism of intermediate-sized circular DNA compaction mediated by spermine: contribution of fluorescence lifetime correlation spectroscopy. J Fluoresc 18(3-4):679-684. doi: 10.1007/s10895-008-0345-y
48. Humpolíčková J, Benda A, Beranová L, Hof M (2009) Compaction mechanism of intermediate-sized DNA elucidated by fluorescence lifetime correlation spectroscopy. Chem Listy 103(11):911-914
49. Benda A, Fagul'ova V, Deyneka A, Enderlein J, Hof M (2006) Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: new perspectives in supported phospholipid bilayer research. Langmuir 22(23):9580-9585. doi: 10.1021/la061573d
50. Ray K, Zhang J, Lakowicz JR (2009) Fluorophore conjugated silver nanoparticles: a time-resolved fluorescence correlation spectroscopic study. Proc Soc Photo Opt Instrum Eng 7185:71850C. doi: 10.1117/12.808958
51. Ray K, Zhang J, Lakowicz JR (2008) Fluorescence lifetime correlation spectroscopic study of fluorophore-labeled silver nanoparticles. Anal Chem 80(19):7313-7318. doi: 10.1021/ac8009356
52. Štefl M, Benda A, Gregor I, Hof M (2014) The fast polarization modulation based dual-focus fluorescence correlation spectroscopy. Opt Express 22(1):885-899. doi: 10.1364/oe.22.000885
53. Dertinger T, Pacheco V, von der Hocht I, Hartmann R, Gregor I, Enderlein J (2007) Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements. ChemPhysChem 8(3):433-443. doi: 10.1002/cphc.200600638
54. Enderlein J, Gregor I, Patra D, Dertinger T, Kaupp UB (2005) Performance of fluorescence correlation spectroscopy for measuring diffusion and concentration. ChemPhysChem 6(11):2324-2336. doi: 10.1002/cphc.200500414
55. Gregor I, Patra D, Enderlein J (2005) Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation. ChemPhysChem 6(1):164-170. doi: 10.1002/cphc.200400319
56. Enderlein J, Gregor I, Patra D, Fitter J (2004) Art and artefacts of fluorescence correlation spectroscopy. Curr Pharm Biotechnol 5(2):155-161. doi: 10.2174/1389201043377020
57. Rüttinger S, Kapusta P, Patting M, Wahl M, Macdonald R (2010) On the resolution capabilities and limits of fluorescence lifetime correlation spectroscopy (FLCS) measurements. J Fluoresc 20(1):105-114. doi: 10.1007/s10895-009-0528-1
58. Rüttinger S, Kapusta P, Patting M, Wahl M, Macdonald R (2009) Comparison of background corrected fluorescence correlation spectroscopy and fluorescence lifetime correlation spectroscopy: dilution series revisited. Proc Soc Photo Opt Instrum Eng 7185:718508. doi: 10.1117/12.808883
59. Enderlein J, Gregor I (2005) Using fluorescence lifetime for discriminating detector afterpulsing in fluorescence-correlation spectroscopy. Rev Sci Instrum 76(3):033102. doi: 10.1063/1.1863399
60. Gärtner M, Mütze J, Ohrt T, Schwille P (2009) Fluorescence lifetime correlation spectroscopy for precise concentration detection in vivo by background subtraction. Proc Soc Photo Opt Instrum Eng 7368:73681V. doi: 10.1117/12.831572
61. Tabarin T, Martin A, Forster RJ, Keyes TE (2012) Poly-ethylene glycol induced super-diffusivity in lipid bilayer membranes. Soft Matter 8(33):8743. doi: 10.1039/c2sm25742d
62. Pembouong G, Morellet N, Kral T, Hof M, Scherman D, Bureau MF, Mignet N (2011) A comprehensive study in triblock copolymer membrane interaction. J Control Release 151(1):57-64. doi: 10.1016/j.jconrel.2011.01.007
63. Yuan CT, Lin CA, Lin TN, Chang WH, Shen JL, Cheng HW, Tang J (2013) Probing the photoluminescence properties of gold nanoclusters by fluorescence lifetime correlation spectroscopy. J Chem Phys 139(23):234311. doi: 10.1063/1.4848695
64. Cui Y, Cho IH, Chowdhury B, Irudayaraj J (2013) Real-time dynamics of methyl-CpG-binding domain protein 3 and its role in DNA demethylation by fluorescence correlation spectroscopy. Epigenetics 8(10):1089-1100. doi: 10.4161/epi.25958
65. Macháň R, Hof M, Chernovets T, Zhmak MN, Ovchinnikova TV, Sýkora J (2011) Formation of arenicin-1 microdomains in bilayers and their specific lipid interaction revealed by Z-scan FCS. Anal Bioanal Chem 399(10):3547-3554. doi: 10.1007/s00216-011-4694-z
66. Breton M, Berret J-F, Bourgaux C, Kral T, Hof M, Pichon C, Bessodes M, Scherman D, Mignet N (2011) Protonation of lipids impacts the supramolecular and biological properties of their self-assembly. Langmuir 27(20):12336-12345. doi: 10.1021/la202439s
67. Macháň R, Miszta A, Hermens W, Hof M (2010) Real-time monitoring of melittin-induced pore and tubule formation from supported lipid bilayers and its physiological relevance. Chem Phys Lipids 163(2):200-206. doi: 10.1016/j.chemphyslip.2009.11.005
68. Krämer B, Buschmann V, Ortmann U, Koberling F, Wahl M, Patting M, Kapusta P, Bülter A, Erdmann R (2008) Advanced FRET and FCS measurements with laser scanning microscopes based on time-resolved techniques. Proc Soc Photo Opt Instrum Eng 6860:68601D. doi: 10.1117/12.761141
69. Basit H, Lopez SG, Keyes TE (2014) Fluorescence correlation and lifetime correlation spectroscopy applied to the study of supported lipid bilayer models of the cell membrane. Methods. doi: 10.1016/j.ymeth.2014.02.005
70. Luchowski R, Gryczynski Z, Sarkar P, Borejdo J, Szabelski M, Kapusta P, Gryczynski I (2009) Instrument response standard in time-resolved fluorescence. Rev Sci Instrum 80(3):033109. doi: 10.1063/1.3095677
71. Szabelski M, Ilijev D, Sarkar P, Luchowski R, Gryczynski Z, Kapusta P, Erdmann R, Gryczynski I (2009) Collisional quenching of erythrosine b as a potential reference dye for impulse response function evaluation. Appl Spectrosc 63(3):363-368. doi: 10.1366/000370209787598979
72. Szabelski M, Luchowski R, Gryczynski Z, Kapusta P, Ortmann U, Gryczynski I (2009) Evaluation of instrument response functions for lifetime imaging detectors using quenched Rose Bengal solutions. Chem Phys Lett 471(1-3):153-159. doi: 10.1016/j.cplett.2009.02.001
73. Luchowski R, Kapusta P, Szabelski M, Sarkar P, Borejdo J, Gryczynski Z, Gryczynski I (2009) Förster resonance energy transfer (FRET)-based picosecond lifetime reference for instrument response evaluation. Meas Sci Technol 20(9):095601. doi: 10.1088/0957-0233/20/9/095601
74. Rich RM, Mummert M, Gryczynski Z, Borejdo J, Gryczynski I, Sørensen TJ, Laursen BW, Fudala R (2013) Detection of hyaluronidase activity using fluorescence lifetime correlation spectroscopy to separate diffusing species and eliminate autofluorescence. Proc Soc Photo Opt Instrum Eng 8590:859003. doi: 10.1117/12.2007261
75. Rich RM, Mummert M, Gryczynski Z, Borejdo J, Sorensen TJ, Laursen BW, Foldes-Papp Z, Gryczynski I, Fudala R (2013) Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAngulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC). Anal Bioanal Chem 405(14):4887-4894. doi: 10.1007/s00216-013-6879-0
76. Schleifenbaum F, Elgass K, Sackrow M, Caesar K, Berendzen K, Meixner AJ, Harter K (2010) Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging: a novel technique for fluorescence microscopy of endogenously expressed fusion-proteins. Mol Plant 3(3):555-562. doi: 10.1093/mp/ssp110
77. Bhatta H, Enderlein J, Rosengarten G (2009) Fluorescence correlation spectroscopy to study diffusion through diatom nanopores. J Nanosci Nanotechnol 9(11):6760-6766. doi: 10.1166/jnn.2009.2020
78. Lamb DC, Schenk A, Röcker C, Nienhaus GU (2000) Determining chemical rate coefficients using time-gated fluorescence correlation spectroscopy. J Phys Org Chem 13(10):654-658. doi: 10.1002/1099-1395(200010)13:10<654::aid- poc294>3.0.co;2-s
79. Lamb DC, Schenk A, Röcker C, Scalfi-Happ C, Nienhaus GU (2000) Sensitivity enhancement in fluorescence correlation spectroscopy of multiple species using time-gated detection. Biophys J 79(2):1129-1138. doi: 10.1016/S0006-3495(00)76366-1
80. Paredes JM, Orte A, Crovetto L, Alvarez-Pez JM, Rios R, Ruedas-Rama MJ, Talavera EM (2010) Similarity between the kinetic parameters of the buffer-mediated proton exchange reaction of a xanthenic derivative in its ground- and excited-state. Phys Chem Chem Phys 12(2):323-327. doi: 10.1039/b917333c
81. Paredes JM, Crovetto L, Rios R, Orte A, Alvarez-Pez JM, Talavera EM (2009) Tuned lifetime, at the ensemble and single molecule level, of a xanthenic fluorescent dye by means of a buffer-mediated excited-state proton exchange reaction. Phys Chem Chem Phys 11(26):5400-5407. doi: 10.1039/b820742a
82. Bestvater F, Seghiri Z, Kang MS, Groner N, Lee JY, Im KB, Wachsmuth M (2010) EMCCD-based spectrally resolved fluorescence correlation spectroscopy. Opt Express 18(23):23818-23828. doi: 10.1364/OE.18.023818
83. Ishii K, Tahara T (2010) Resolving inhomogeneity using lifetime-weighted fluorescence correlation spectroscopy. J Phys Chem B 114(38):12383-12391. doi: 10.1021/jp104234c
84. Yang H, Xie XS (2002) Probing single-molecule dynamics photon by photon. J Chem Phys 117(24):10965-10979. doi: 10.1063/1.1521154
85. Yang H, Xie XS (2002) Statistical approaches for probing single-molecule dynamics photon-by-photon. Chem Phys 284(1-2):423-437. doi: 10.1016/s0301- 0104(02)00672-9
86. Ishii K, Tahara T (2012) Extracting decay curves of the correlated fluorescence photons measured in fluorescence correlation spectroscopy. Chem Phys Lett 519-520:130-133. doi: 10.1016/j.cplett.2011.11.024
87. Ishii K, Tahara T (2013) Two-dimensional fluorescence lifetime correlation spectroscopy. 2. Application. J Phys Chem B. doi: 10.1021/jp406864e
88. Ishii K, Tahara T (2013) Two-dimensional fluorescence lifetime correlation spectroscopy. 1. Principle. J Phys Chem B. doi: 10.1021/jp406861u
89. Anthony N, Berland K (2013) Chapter Seven - Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy. In: Sergey YT (ed) Methods in Enzymology, vol Volume 518. Academic Press, pp 145-173. doi: 10.1016/B978-0-12-388422-0.00007-8
90. Berland KM, Anthony NR (2013) Enhanced resolution and sensitivity in fluorescence fluctuation measurements using multi-modal data acquisition and global analysis. Proc Soc Photo Opt Instrum Eng 8588:858801. doi: 10.1117/12.2008872
91. Guo P (2009) Lifetime Resolved Fluorescence Correlation Spectroscopy and Two-Photon Spectroscopy of Amyloid Nanotube Bundles. Dissertation, Emory University, Atlanta
92. Anthony NR, Berland KM (2014) tauFCS: Multi-Method Global Analysis Enhances Resolution and Sensitivity in Fluorescence Fluctuation Measurements. PLoS One 9(2):e90456. doi: 10.1371/journal.pone.0090456
93. Elgass K, Caesar K, Harter K, Meixner AJ, Schleifenbaum F (2011) Combining ocFLIM and FIDSAM reveals fast and dynamic physiological responses at subcellular resolution in living plant cells. J Microsc 242(2):124-131. doi: 10.1111/j.1365-2818.2010.03446.x
94. Elgass K, Caesar K, Wanke D, Harter K, Meixner AJ, Schleifenbaum F (2010) Application of FLIM-FIDSAM for the in vivo analysis of hormone competence of different cell types. Anal Bioanal Chem 398(5):1919-1925. doi: 10.1007/s00216-010-4127-4
95. Pattern Matching Analysis - Advanced FLIM analysis feature of the SymPhoTime 64 software. (2014) PicoQuant. http://picoquant.com/applications/ category/life-science/pattern-matching-analysis. Accessed 17 Jan 2014
96. Gregor I, Krämer B, Koberling F, Erdmann R, Enderlein J, Wahl M, Fore S (2011) Fast algorithms for the analysis of spectral FLIM data. Proc Soc Photo Opt Instrum Eng 7903:790330. doi: 10.1117/12.873386
97. Kumar ATN, Chung E, Raymond SB, van de Water JAJM, Shah K, Fukumura D, Jain RK, Bacskai BJ, Boas DA (2009) Feasibility of in vivo imaging of fluorescent proteins using lifetime contrast. Opt Lett 34(13):2066-2068. doi: 10.1364/ol.34.002066
98. Zimmermann T, Rietdorf J, Pepperkok R (2003) Spectral imaging and its applications in live cell microscopy. FEBS Lett 546(1):87-92. doi: 10.1016/S0014-5793(03)00521-0
99. Neher RA, Mitkovski M, Kirchhoff F, Neher E, Theis FJ, Zeug A (2009) Blind source separation techniques for the decomposition of multiply labeled fluorescence images. Biophys J 96(9):3791-3800. doi: 10.1016/j.bpj.2008.10.068
100. Zimmermann T, Marrison J, Hogg K, O'Toole P (2014) Clearing up the signal: spectral imaging and linear unmixing in fluorescence microscopy. In: Paddock SW (ed) Confocal microscopy, vol 1075. Methods in molecular biology. Springer, New York, pp 129-148
101. Muñoz-Barrutia A, García-Muñoz J, Ucar B, Fernández-García I, Ortiz-De-Solorzano C Blind Spectral Unmixing of M-FISH Images by Non-negative Matrix Factorization. In: Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE, 22-26 Aug. 2007. pp 6247-6250. doi: 10.1109/iembs.2007. 4353783
102. Digman MA, Caiolfa VR, Zamai M, Gratton E (2008) The phasor approach to fluorescence lifetime imaging analysis. Biophys J 94(2):L14-L16. doi: 10.1529/biophysj.107.120154
103. Štefl M, Šachl R, Humpolíčková J, Cebecauer M, Macháň R, Kolářová M, Johansson LB, Hof M (2012) Dynamics and size of cross-linking-induced lipid nanodomains in model membranes. Biophys J 102(9):2104-2113. doi: 10.1016/j.bpj.2012.03.054
104. Fereidouni F, Bader AN, Gerritsen HC (2012) Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images. Opt Express 20(12):12729-12741. doi: 10.1364/oe.20.012729
105. Kolin DL, Wiseman PW (2007) Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells. Cell Biochem Biophys 49(3):141-164. doi: 10.1007/s12013-007-9000-5
106. Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J (2009) Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI). Proc Natl Acad Sci U S A 106(52):22287-22292. doi: 10.1073/pnas.0907866106
107. Dertinger T, Colyer R, Vogel R, Enderlein J, Weiss S (2010) Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI). Opt Express 18(18):18875-18885. doi: 10.1364/oe.18.018875
108. van den Bogaart G, Kusters I, Velasquez J, Mika JT, Krasnikov V, Driessen AJ, Poolman B (2008) Dual-color fluorescence-burst analysis to study pore formation and protein-protein interactions. Methods 46(2):123-130. doi: 10.1016/j.ymeth.2008.06.016