Pulsed interleaved excitation

Biophysical Journal

Volumn 89, Issue 5, 2005, Pages 3508-3522

  Müller, Barbara K ^a   Zaychikov, Evgeny ^b   Bräuchle, Christoph ^a   Lamb, Don C ^a,c  

^a UNIVERSITY OF MUNICH   (Germany)

^b MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^c University of Illinois at Urbana Champaign   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA;

ARTICLE; CALIBRATION; COLOR; CONFOCAL MICROSCOPY; CONTROLLED STUDY; FLUORESCENCE; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE IMAGING; FLUORESCENCE RESONANCE ENERGY TRANSFER; HELA CELL; HISTOGRAM; HUMAN; HUMAN CELL; PHOTON; QUANTITATIVE ANALYSIS; SIGNAL NOISE RATIO;

EID: 27744519499     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.105.064766     Document Type: Article

References (51)

1. Magde, D., E. L. Elson, and W. W. Webb. 1972. Thermodynamic fluctuations in a reacting system: measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
2. Elson, E. L., and D. Magde. 1974. Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers. 13:1-27. Biopolymers. 13:1-27.
3. Schwille, P., J. Bieschke, and F. Oehlenschlager. 1997. Kinetic investigations by fluorescence correlation spectroscopy: the analytical and diagnostic potential of diffusion studies. Biophys. Chem. 66:211-228.
4. Schwille, P., F. J. Meyer-Almes, and R. Rigler. 1997. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys. J. 72:1878-1886.
5. Eggeling, C., J. R. Fries, L. Brand, R. Gunther, and C. A. M. Seidel. 1998. Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy. Proc. Natl. Acad. Sci. USA. 95:1556-1561.
6. Yildiz, A., J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin. 2003. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science. 300:2061-2065.
7. Ha, T., T. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss. 1996. Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proc. Natl. Acad. Sci. USA. 93:6264-6268.
8. Deniz, A. A., M. Dahan, J. R. Grunwell, T. J. Ha, A. E. Faulhaber, D. S. Chemla, S. Weiss, and P. G. Schultz. 1999. Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Forster distance dependence and subpopulations. Proc. Natl. Acad. Sci. USA. 96:3670-3675.
9. Michalet, X., and S. Weiss. 2002. Single-molecule spectroscopy and microscopy. C. R. Phys. 3:619-644.
10. Schuler, B., E. A. Lipman, and W. A. Eaton. 2002. Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy. Nature. 419:743-747.
11. Lipman, E. A., B. Schuler, O. Bakajin, and W. A. Eaton. 2003. Single-molecule measurement of protein folding kinetics. Science. 301:1233-1235.
12. Seisenberger, G., M. U. Ried, T. Endreß, H. Büning, M. Hallek, and C. Bräuchle. 2001. Real-time single molecule imaging of the infection pathway of an adeno-associated virus. Science. 294:1929-1932.
13. Brauchle, C., G. Seisenberger, T. Endreß, M. U. Ried, H. Büning, and M. Hallek. 2002. Single virus tracing: visualization of the infection pathway of a virus into a living cell. ChemPhysChem. 3:299-303.
14. Lamb, D. C., A. Schenk, C. Röcker, and G. U. Nienhaus. 2000. Determining chemical rate coefficients using time-gated fluorescence correlation spectroscopy. J. Phys. Org. Chem. 13:654-658.
15. Eggeling, C., S. Berger, L. Brand, J. R. Fries, J. Schaffer, A. Volkmer, and C. A. Seidel. 2001. Data registration and selective single-molecule analysis using multi-parameter fluorescence detection. J. Biotechnol. 86:163-180.
16. Lamb, D. C., A. Schenk, C. Röcker, C. Scalfi-Happ, and G. U. Nienhaus. 2000. Sensitivity enhancement in fluorescence correlation spectroscopy of multiple species using time-gated detection. Biophys. J. 79:1129-1138.
17. Bohmer, M., M. Wahl, H. J. Rahn, R. Erdmann, and J. Enderlein. 2002. Time-resolved fluorescence correlation spectroscopy. Chem. Phys. Lett. 353:439-445.
18. Kuhnemuth, R., and C. A. M. Seidel. 2001. Principles of single molecule multiparameter fluorescence spectroscopy. Single Molecules. 2:251-254.
19. Rothwell, P. J., S. Berger, O. Kensch, S. Felekyan, M. Antonik, B. M. Wohrl, T. Restle, R. S. Goody, and C. A. M. Seidel. 2003. Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes. Proc. Natl. Acad. Sci. USA. 100:1655-1660.
20. Kapanidis, A. N., N. K. Lee, T. A. Laurence, S. Doose, E. Margeat, and S. Weiss. 2004. Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules. Proc. Natl. Acad. Sci. USA. 101:8936-8941.
21. Lee, N. K., A. N. Kapanidis, Y. Wang, X. Michalet, J. Mukhopadhyay, R. H. Ebright, and S. Weiss. 2005. Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation. Biophys. J. 88:2939-2953.
22. Magde, D., E. L. Elson, and W. W. Webb. 1974. Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers. 13:29-61.
23. Ehrenberg, M., and R. Rigler. 1974. Rotational Brownian motion and fluorescence intensity fluctuations. Chem. Phys. 4:390-401.
24. Aragón, S. F., and R. Pecora. 1976. Fluorescence correlation spectroscopy as a probe of molecular dynamics. J. Chem. Phys. 64:1791-1803.
25. Kask, P., P. Piksarv, M. Pooga, Ü. Mets, and E. Lippmaa. 1989. Separation of the rotational contribution in fluorescence correlation experiments. Biophys. J. 55:213-220.
26. Rauer, B., E. Neumann, J. Widengren, and R. Rigler. 1996. Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin a-bungarotoxin with Torpedo californica acetylcholine receptor. Biophys. Chem. 58:3-12.
27. Bismuto, E., E. Gratton, and D. C. Lamb. 2001. Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy. Biophys. J. 81:3510-3521.
28. Widengren, J., R. Rigler, and Ü. Mets. 1994. Triplet-state monitoring by fluorescence correlation spectroscopy. J. Fluoresc. 4:255-258.
29. Widengren, J., Ü. Mets, and R. Rigler. 1995. Fluorescence correlation spectroscopy of triplet states in solution: a theoretical and experimental study. J. Phys. Chem. 99:13368-13379.
30. Bonnet, G., O. Krichevsky, and A. Libchaber. 1998. Kinetics of conformational fluctuations in DNA hairpin-loops. Proc. Natl. Acad. Sci. USA. 95:8602-8606.
31. Haupts, U., S. Maiti, P. Schwille, and W. W. Webb. 1998. Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy. Proc. Natl. Acad. Sci. USA. 95:13573-13578.
32. Rigler, R., P. Kask, Ü. Mets, and J. Widengren. 1993. Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion. Eur. Biophys. J. 22: 169-175.
33. Eigen, M., and R. Rigler. 1994. Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc. Natl. Acad. Sci. USA. 91:5740-5747.
34. Thompson, N. L. 1991. Fluorescence correlation spectroscopy. In Topics in Fluorescence Spectroscopy: Techniques, Vol. 1. J. R. Lakowicz, editor. Plenum Press, New York. 337-378.
35. Hess, S. T., S. H. Huang, A. A. Heikal, and W. W. Webb. 2002. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry. 41:697-705.
36. Haustein, E., and P. Schwille. 2003. Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods. 29:153-166.
37. Kettling, U., A. Koltermann, P. Schwille, and M. Eigen. 1998. Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc. Natl. Acad. Sci. USA. 95:1416-1420.
38. Thews, E., M. Gerken, R. Eckert, J. Zapfel, C. Tietz, and J. Wrachtrup. 2005. Cross talk free fluorescence cross-correlation spectroscopy in live cells. Biophys. J. 89:2069-2076.
39. Förster, T. 1948. Zwischenmolekulare energiewanderung und fluoreszenz. Ann. Physik (Leipzig) [in German]. 2:55-75.
40. Clegg, R. M., A. I. H. Murchie, A. Zechel, and D. M. J. Lilley. 1993. Observing the helical geometry of double-stranded DNA in solution by fluorescence resonance energy-transfer. Proc. Natl. Acad. Sci. USA. 90:2994-2998.
41. Clegg, R. 1995. Fluorescence energy transfer microscopy. In Fluorescence Imaging Spectroscopy and Microscopy. X. F. Wang and B. Herman, editors. John Wiley & Sons, New York, NY. 170-252.
42. Selvin, P. R. 1995. Fluorescence resonance energy transfer. Methods Enzymol. 245:300-334.
43. Kohl, T., K. G. Heinze, R. Kuhlemann, A. Koltermann, and P. Schwille. 2002. A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins. Proc. Natl. Acad. Sci. USA. 99:12161-12166.
44. Widengren, J., E. Schweinberger, S. Berger, and C. A. M. Seidel. 2001. Two new concepts to measure fluorescence resonance energy transfer via fluorescence correlation spectroscopy. Theory and Experimental Realizations. J. Phys. Chem. A. 105:6851-6866.
45. Boeckle, S., K. von Gersdorff, S. van der Piepen, C. Culmsee, E. Wagner, and M. Ogris. 2004. Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer. J. Gene Med. 6:1102-1111.
46. Basselet, S., E. J. G. Peterman, A. Mityawaki, and W. E. Moerner. 2000. Single-molecule fluorescence resonant energy transfer in calcium concentration dependent cameleon. J. Phys. Chem. B. 104:3676-3682.
47. Gregor, I., D. Patra, and J. Enderlein. 2005. Optical saturation in fluorescence correlation spectroscopy under continuous-wave and pulsed excitation. ChemPhysChem. 6:164-170.
48. Chen, Y., J. D. Müller, P. T. C. So, and E. Oration. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:553-567.
49. Kask, P., K. Palo, D. Ullmann, and K. Gall. 1999. Fluorescence-intensity distribution analysis and its application in biomolecular detection technology. Proc. Natl. Acad. Sci. USA. 94:13756-13761.
50. Haustein, E., M. Jahnz, and P. Schwille. 2003. Triple FRET: a tool for studying long-range molecular interactions. ChemPhysChem. 4:745-748.
51. Hohng, S., C. Joo, and T. Ha. 2004. Single-molecule three-color FRET. Biophys. J. 87:1328-1337.