Combining ocFLIM and FIDSAM reveals fast and dynamic physiological responses at subcellular resolution in living plant cells

Journal of Microscopy

Volumn 242, Issue 2, 2011, Pages 124-131

  Elgass, K ^a   Caesar, K ^a   Harter, K ^a   Meixner, A J ^a   Schleifenbaum, F ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

EGFP; FIDSAM; Fluorescence lifetime; LTI6b; OcFLIM

Indexed keywords

CELLS; CYTOLOGY; FLUORESCENCE IMAGING; PHYSIOLOGICAL MODELS; PLANTS (BOTANY); PROTEINS; TEMPERATURE;

EGFP; FIDSAM; FLUORESCENCE LIFETIMES; INTRACELLULAR PROCESS; LTI6B; MOLECULAR MECHANISM; OCFLIM; PHYSIOLOGICAL RESPONSE; PLANTS CELLS; SUBCELLULAR RESOLUTION;

CHROMOPHORES;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARABIDOPSIS; ARTICLE; AUTOFLUORESCENCE; CELL MEMBRANE; CELLULAR DISTRIBUTION; FLUORESCENCE; FLUORESCENCE INTENSITY DECAY SHAPE ANALYSIS MICROSCOPY; HYPERPOLARIZATION; IMAGING; LOW TEMPERATURE; MICROSCOPY; NONHUMAN; ONE CHROMOPHORE FLUORESCENCE LIFETIME IMAGING MICROSCOPY; PLANT CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SALT STRESS; SIGNAL TRANSDUCTION;

ARABIDOPSIS;

EID: 79953306240     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2010.03446.x     Document Type: Article

References (27)

1. Allen, G.J., Chu, S.P., Harrington, C.L. et al (2001) A defined range of guard cell calcium oscillation parameters encodes stomatal movements. Nature 411, 1053-1057.
2. Blum, C., Stracke, F., Becker, S., Mullen, K. & Meixner, A.J. (2001) Discrimination and interpretation of spectral phenomena by room-temperature single-molecule spectroscopy. J. Phys. Chem. A 105, 6983-6990.
3. Cutler, S.R., Ehrhardt, D.W., Griffitts, J.S. & Somerville, C.R. (2000) Random GFP:: cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc. Natl. Acad. Sci. USA 97, 3718-3723.
4. Dettmer, J., Hong-Hermesdorf, A., Stierhof, Y.D. & Schumacher, K. (2006) Vacuolar H+-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis. Plant Cell 18, 715-730.
5. Elgass, K., Caesar, K., Schleifenbaum, F., Stierhof, Y.-D., Meixner, A.J. & Harter, K. (2009) Novel application of fluorescence lifetime and fluorescence microscopy enables quantitative access to subcellular dynamics in plant cells. PLoS ONE 4, e5716, 1-13.
6. Elgass, K., Caesar, K., Schleifenbaum, F., Meixner, A.J. & Harter, K. (2010) The fluorescence lifetime of BRI1-GFP as probe for the noninvasive determination of the membrane potential in living cells. Proc. SPIE 7568, 1-8.
7. Grabov, A. & Blatt, M.R. (1998) Membrane voltage initiates Ca2+ waves and potentiates Ca2+ increases with abscisic acid in stomatal guard cells. Proc. Natl. Acad. Sci. USA 95, 4778-4783.
8. Hink, M.A., Shah, K., Russinova, E., de Vries, S.C. & Visser, A. (2008) Fluorescence fluctuation analysis of Arabidopsis thaliana somatic embryogenesis receptor-like kinase and brassinosteroid insensitive 1 receptor oligomerization. Biophys. J. 94, 1052-1062.
9. Horcas, I., Fernandez, R., Gomez-Rodriguez, J.M., Colchero, J., Gomez-Herrero, J. & Baro, A.M. (2007) WSXM: a software for scanning probe microscopy and a tool for nanotechnology. Rev. Sci. Instrum. 78, 013705-1-013705-8.
10. Kim, S.H., Kim, J.Y., Kim, S.J., An, K.S., An, G. & Kim, S.R. (2007) Isolation of cold stress-responsive genes in the reproductive organs, and characterization of the OsLti6b gene from rice (Oryza sativa L.). Plant Cell Rep. 26, 1097-1110.
11. Kriegsmann, J., Gregor, I., von der Hocht, I., Klare, J.P., Engelhard, M., Enderlein, J. & Fitter, J. (2009) Translational diffusion and interaction of a photoreceptor and its cognate transducer observed in giant unilamellar vesicles by using dual-focus FCS. ChemBioChem 10, 1823-1829.
12. Lakowicz, J.R. (2006) Principles of Fluorescence Spectroscopy. Kluwer Academic, Plenum Publishers, New York.
13. Martini, J., Schmied, K., Palmisano, R., Toensing, K., Anselmetti, D. & Merkle, T. (2007) Multifocal two-photon laser scanning microscopy combined with photo-activatable GFP for in vivo monitoring of intracellular protein dynamics in real time. J. Struct. Biol. 158, 401-409.
14. Medina, J., Catala, R. & Salinas, J. (2001) Developmental and stress regulation of RCI2A and RCI2B, two cold-inducible genes of arabidopsis encoding highly conserved hydrophobic proteins. Plant Physiol. 125, 1655-1666.
15. Medina, J., Ballesteros, M.L. & Salinas, J. (2007) Phylogenetic and functional analysis of Arabidopsis RCI2 genes. J. Exp. Bot. 58, 4333-4346.
16. Mitsuya, S., Taniguchi, M., Miyake, H. & Takabe, T. (2005) Disruption of RCI2A leads to over-accumulation of Na+ and increased salt sensitivity in Arabidopsis thaliana plants. Planta 222, 1001-1009.
17. Mitsuya, S., Taniguchi, M., Miyake, H. & Takabe, T. (2006) Overexpression of RC12A decreases Na+ uptake and mitigates salinity-induced damages in Arabidopsis thaliana plants. Physiol. Plant. 128, 95-102.
18. Nakabayashi, T., Wang, H.P., Kinjo, M. & Ohta, N. (2008) Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements. Photochem. Photobiol. Sci. 7, 668-670.
19. Navarre, C. & Goffeau, A. (2000) Membrane hyperpolarization and salt sensitivity induced by deletion of PMP3, a highly conserved small protein of yeast plasma membrane. EMBO J. 19, 2515-2524.
20. Nylander, M., Heino, P., Helenius, E., Palva, E.T., Ronne, H. & Welin, B.V. (2001) The low-temperature- and salt-induced RCI2A gene of Arabidopsis complements the sodium sensitivity caused by a deletion of the homologous yeast gene SNA1. Plant Mol. Biol. 45, 341-352.
21. Ries, J. & Schwille, P. (2008) New concepts for fluorescence correlation spectroscopy on membranes. Phys. Chem. Chem. Phys. 10, 3487-3497.
22. Schleifenbaum, F., Blum, C., Elgass, K., Subramaniam, V. & Meixner, A.J. (2008) New insights into the photophysics of DsRed by multiparameter spectroscopy on single proteins. J. Phys. Chem. B 112, 7669-7674.
23. Schleifenbaum, F., Elgass, K., Sackrow, M., Caesar, K., Berendzen, K., Meixner, A.J. & Harter, K. (2009) Fluorescence intensity decay shape analysis microscopy (FIDSAM) for quantitative and sensitive live-cell imaging. Mol. Plant 3(3), 555-562.
24. Toyooka, K., Goto, Y., Asatsuma, S., Koizumi, M., Mitsui, T. & Matsuoka, K. (2009) A mobile secretory vesicle cluster involved in mass transport from the golgi to the plant cell exterior. Plant Cell 21, 1212-1229.
25. van Manen, H.J., Verkuijlen, P., Wittendorp, P., Subramaniam, V., van den Berg, T.K., Roos, D. & Otto, C. (2008) Refractive index sensing of green fluorescent proteins in living cells using fluorescence lifetime imaging microscopy. Biophys. J. 94, L67-L69.
26. Westphal, V., Rizzoli, S.O., Lauterbach, M.A., Kamin, D., Jahn, R. & Hell, S.W. (2008) Video-rate far-field optical nanoscopy dissects synaptic vesicle movement. Science 320, 246-249.
27. Willig, K.I., Rizzoli, S.O., Westphal, V., Jahn, R. & Hell, S.W. (2006) STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440, 935-939.