Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM)

Biophysical Journal

Volumn 83, Issue 3, 2002, Pages 1631-1649

  Clayton, Andrew H A ^a,b,c   Hanley, Quentin S ^a,b,c   Arndt Jovin, Donna J ^a,b,c   Subramaniam, Vinod ^a,b,c   Jovin, Thomas M ^a,b,c,d  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b ROYAL MELBOURNE HOSPITAL   (Australia)

^c UNIVERSITY OF THE WEST INDIES   (Jamaica)

^d ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCEROL; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

ANISOTROPY; ARTICLE; CHROMATOPHORE; DEPOLARIZATION; ENERGY TRANSFER; EXCITATION; FLUORESCENCE; FLUORESCENCE ANISOTROPY IMAGING MICROSCOPY; FLUOROPHORE; FREQUENCY DISCRIMINATION; MATHEMATICAL ANALYSIS; MICROSCOPY; MOLECULAR DYNAMICS; MOLECULAR MODEL; OPTICAL ROTATION; POLARIZATION; PROTEIN EXPRESSION; PROTEIN STRUCTURE; BIOPHYSICS; CHEMISTRY; DOSE RESPONSE; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; INSTRUMENTATION; METABOLISM; METHODOLOGY; THEORETICAL MODEL; ULTRASTRUCTURE;

ANISOTROPY; BIOPHYSICS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; GLYCEROL; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, VIDEO; MODELS, THEORETICAL;

EID: 0036708469     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)73932-5     Document Type: Article

References (70)

1. Polarisations and assignments of transitions: The method of photoselection
2. Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarisation
3. Fluorescence polarisation microscopy
4. Depolarization by high aperture focusing
5. Detection of receptor clustering by flow cytometric fluorescence anisotropy measurements
6. Oligomeric state of human eythrocyte Band 3 measured by fluorescence resonance energy homotransfer
7. Energy transfer and migration in fluorescent solutions
8. Time-resolved polarization imaging by pump-probe (stimulated emission) fluorescence microscopy
9. Internal dynamics and overall motion of lysozyme studied by fluorescence depolarization of the eosin lysozyme complex
10. Analysis of the backbone dynamics of interleukin-1-beta using two-dimensional inverse detected heteronuclear N-15 H-1 NMR spectroscopy
11. Whole-field optically sectioned fluorescence lifetime imaging
12. Excited-state dynamics in the enhanced green fluorescent protein mutant probed by picosecond time-resolved single photon counting spectroscopy
13. Influence of inhibitor binding on the internal motions of lysozyme
14. Multiparameter image cytometry: From confocal micrographs to subcellular fluorograms
15. Multicolour analysis and local image correlation in confocal microscopy
16. Mapping of fluorescence anisotropy in living cells by ratio imaging
17. Ultrafast dynamics microscopy
18. Fluorescence anisotropy of rotating molecules in the presence of energy migration
19. Direct observation of rotational diffusion by picosecond spectroscopy
20. Phospholipid order in gel- and fluid-phase cell-size liposomes measured by digitized video fluorescence polarization microscopy
21. Cell-membrane fluidity in the intact kidney proximal tubule measured by orientation-independent fluorescence anisotropy imaging
22. Fluorescence lifetime imaging microscopy (FLIM)-spatial-resolution of microstructures on the nanosecond time-scale
23. Backbone flexibility of five sites on the catalytic subunit of CAMP-dependant protein kinase in the open and closed conformations
24. Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins
25. Fluorescence lifetime measurement using a novel fibreoptic laser scanning confocal microscope
26. Polarization spectroscopy of single fluorescent molecules
27. Fluorescence lifetime imaging: Multi-point calibration, minimum resolvable differences, and artifact suppression
28. Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP); acid-base specificity
29. On the notion of the emission anisotropy
30. EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
31. Fluorescence investigations of the elongation factor TU system
32. Fluorescence anisotropy applied to biomolecular interactions
33. Fluorescence polarization and energy transfer: Theory and application
34. On the wobbling-incone analysis of fluorescence anisotropy decay
35. Differential polarized phase fluorometric investigations of diphenylhexatriene in lipid bilayers. Quantitation of hindered depolarizing rotations
36. Theory and application of differential polarized phase fluorometry
37. Theory of phase-modulation fluorescence spectroscopy for excited-state processes
38. Fluorescence lifetime imaging
39. Effect of librational motion on fluorescence depolarization and nuclear magnetic relaxation data in macromolecules and membranes
40. Solvent dependence of the fluorescence lifetimes of xanthene dyes
41. Rotational anisotropy and solvent-fluophore bonds: An investigation by differential polarized phase fluorometry
42. Subnanosecond polarised microfluorimetry in the time domain: An instrument for studying receptor trafficking in live cells
43. Time resolved imaging microscopy-phosphorescence and delayed fluorescence imaging
44. Applications of FCS to protein-ligand interactions: Comparison with fluorescence polarization
45. Monitoring protein conformation and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein
46. Subtraction of background fluorescence in multiharmonic frequency-domain fluorimetry
47. Rapid diffusion of green fluorescent protein in the mitochondrial matrix
48. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy
49. Förster distances between green fluorescent protein pairs
50. Time-resolved fluorescence imaging and background rejection by two-photon excitation in laser-scanning microscopy
51. Picosecond rotational diffusion in kinetic and steady-state fluorescence spectroscopy
52. Rapid acquisition, analysis and display of fluorescence lifetime-resolved images for real-time applications
53. Application of fluorescence lifetime imaging microscopy to the investigation of intracellular PDT mechanisms
54. An electromagnetic theory of imaging in fluorescence microscopy, and imaging in polarization fluorescence microscopy
55. Multiple-frequency fluorescence lifetime imaging microscopy
56. Photophysics of green and red fluorescent proteins: Some implications for quantitative microscopy
57. Demonstration of an associated anisotropy decay by frequency-domain fluorometry
58. Segmental dynamics of the cytoplasmic domain of erythrocyte band 3 determined by time-resolved fluorescence anisotropy: Sensitivity to pH and ligand binding
59. Restrained torsional dynamics of nuclear DNA in living proliferative mammalian cells
60. GPI-anchored proteins are organized in submicron domains at the cell surface
61. Backbone dynamics of Tet repressor α8nα9 loop
62. Construction and evaluation of a frequency-domain epifluorescence microscope for lifetime and anisotropy decay measurements in subcellular domains
63. Global analysis of fluorescence lifetime imaging microscopy data
64. One- and two-photon excited fluorescence lifetimes and anisotropy decays of green fluorescent proteins
65. Fluorescence pattern analysis based on the time-resolved method
66. Theory of differential phase fluorometry: Detection of anisotropic molecular rotations
67. Limited rotational motion: Recognition by differential phase fluorometry
68. Fluidity, dynamics and order