Quantitative Study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy

Methods in Molecular Biology

Volumn 1076, Issue , 2014, Pages 683-698

  Padilla Parra, Sergi ^a   Audugé, Nicolas ^b   Coppey Moisan, Maïté ^b   Tramier, Marc ^a  

^a UNIVERSITÉ DE RENNES 1   (France)

^b INSTITUT JACQUES MONOD   (France)

Author keywords

FCCS; FCS; FLCS; Quantitative fluorescence microscopy; TCSPC

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; PROTEIN A; RED FLUORESCENT PROTEIN;

ARTICLE; BLEACHING; CONTROLLED STUDY; DIFFUSION COEFFICIENT; FEMALE; FLUORESCENCE CORRELATION SPECTROSCOPY; HISTOGRAM; HUMAN; HUMAN CELL; PHOTON; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; QUANTITATIVE STUDY; WATER IMMERSION;

DIFFUSION; FLUORESCENCE; FLUORESCENT DYES; HUMANS; PROTEIN BINDING; PROTEIN INTERACTION MAPS; PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 84934443967     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-649-8_31     Document Type: Article

References (20)

1. Bacia K, Majoul IV, Schwille P (2002) Probing the endocytic pathway in live cells using dualcolor fluorescence cross-correlation analysis. Biophys J 83:1184-1193
2. Maeder CI, Hink MA, Kinkhabwala A et al (2007) Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol 9:1319-1326
3. Liu P, Sudhaharan T, Koh RM et al (2007) Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence crosscorrelation spectroscopy. Biophys J 93:684-698
4. Lillemeier BF, Mörtelmaier MA, Forstner MB et al (2010) TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation. Nat Immunol 11:90-96
5. Huet S, Avilov SV, Ferbitz L et al (2010) Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy. J Virol 84:1254-1264
6. Bacia K, Schwille P (2007) Practical guidelines for dual-color fluorescence cross-correlation spectroscopy. Nat Protoc 2:2842-2856
7. Kogure T, Karasawa S, Araki T et al (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color singlelaser fluorescence cross-correlation spectroscopy. Nat Biotechnol 24:577-581
8. Shcherbakova DM, Hink MA, Joosen L et al (2012) An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging. J Am Chem Soc 134:7913-7923
9. Muller BK, Zaychikov E, Brauchle C, Lamb DC (2005) Pulsed interleaved excitation. Biophys J 89:3508-3522
10. Thews E,GerkenM, Eckert R et al (2005) Cross talk free fluorescence cross correlation spectroscopy in live cells. Biophys J 89:2069-2076
11. Takahashi Y, Nishimura J, Suzuki A et al (2008) Cross-talk-free fluorescence crosscorrelation spectroscopy by the switching method. Cell Struct Funct 33:143-150
12. Ries J, Yu SR, Burkhardt M et al (2009) Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms. Nat Methods 6:643-645
13. Böhmer M, Enderlein J (2003) Fluorescence spectroscopy of single molecules under ambient conditions: methodology and technology. Chemphyschem 4:793-808
14. Kapusta P,Wahl M, Benda A et al (2007) Fluorescence lifetime correlation spectroscopy. J Fluoresc 17:43-48
15. Gregor I, Enderlein J (2007) Time-resolved methods in biophysics. Fluorescence lifetime correlation spectroscopy. Photochem Photobiol Sci 6:13-18
16. Ray K, Zhang J, Lakowicz JR (2008) Fluorescence lifetime correlation spectroscopic study of fluorophore-labeled silver nanoparticles. Anal Chem 80:7313-7318
17. Ruttinger S, Kapusta P, Patting M et al (2010) On the resolution capabilities and limits of fluorescence lifetime correlation spectroscopy (FLCS) measurements. J Fluoresc 20: 105-114
18. Chen J, Irudayaraj J (2010) Fluorescence lifetime cross correlation spectroscopy resolves EGFR and antagonist interaction in live cells. Anal Chem 82:6415-6421
19. Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M (2011) Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell. Microsc Res Tech 74:788-793
20. Michelman-Ribeiro A, Mazza D, Rosales T et al (2009) Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy. Biophys J 97(1):337-346