Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

Protein Science

Volumn 23, Issue 6, 2014, Pages 769-789

  Yang, Zhengrong ^a   Wang, Chi ^b   Zhou, Qingxian ^a   An, Jianli ^a   Hildebrandt, Ellen ^c   Aleksandrov, Luba A ^d,e   Kappes, John C ^f,g   DeLucas, Lawrence J ^a   Riordan, John R ^d,e   Urbatsch, Ina L ^c,h   Hunt, John F ^b   Brouillette, Christie G ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

^c TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^e UNIVERSITY OF NORTH CAROLINA   (United States)

^f University of Alabama at Birmingham   (United States)

^g BIRMINGHAM VA MEDICAL CENTER   (United States)

^h TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

CD; CFTR; Detergent interaction; DSC; Extramembrane domain; Membrane protein; NBD1; Soluble domain; Thermal unfolding

Indexed keywords

CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DETERGENT; LIPID; MEMBRANE PROTEIN; MONOMER;

ARTICLE; CIRCULAR DICHROISM; CRYSTALLIZATION; DENATURATION; DIFFERENTIAL SCANNING CALORIMETRY; MICELLE; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SPECTROSCOPY; THERMODYNAMICS; CHEMISTRY; METABOLISM; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE;

CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; DETERGENTS; MEMBRANE PROTEINS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 84904174102     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2460     Document Type: Article

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