Mg2+-dependent ATP occlusion at the first nucleotide-binding domain (NBD1) of CFTR does not require the second (NBD2)

Biochemical Journal

Volumn 416, Issue 1, 2008, Pages 129-135

  Aleksandrov, Luba ^a   Aleksandrov, Andrei ^a   Riordan, John R ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

ATP occlusion; ATP binding cassette transporter protein (ABC protein); Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator (CFTR); Nucleotide sandwich

Indexed keywords

ATP BINDING; ATP HYDROLYSIS; ATP OCCLUSION; ATP-BINDING CASSETTE TRANSPORTER PROTEIN (ABC PROTEIN); BINDING POCKETS; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (CFTR); CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATORS; NO REDUCTION; NUCLEOTIDE-BINDING DOMAIN; P-GLYCOPROTEIN; TRANSIENT INTERMEDIATE; WILD TYPES;

CELL MEMBRANES; GLYCOPROTEINS; HYDROLYSIS; POSITIVE IONS;

NUCLEOTIDES;

ADENOSINE TRIPHOSPHATE; MAGNESIUM ION; TRANSMEMBRANE CONDUCTANCE REGULATOR; 8 AZIDOADENOSINE TRIPHOSPHATE; 8-AZIDOADENOSINE 5'-TRIPHOSPHATE; AZIDE; DRUG DERIVATIVE; MAGNESIUM;

ANIMAL CELL; ARTICLE; CHANNEL GATING; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN DOMAIN; TEMPERATURE DEPENDENCE; ANIMAL; CELL CULTURE; COLD; DIMERIZATION; HAMSTER; METABOLISM; PROTEIN TERTIARY STRUCTURE;

ADENOSINE TRIPHOSPHATE; ANIMALS; AZIDES; CELLS, CULTURED; COLD; CRICETINAE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; DIMERIZATION; MAGNESIUM; PROTEIN STRUCTURE, TERTIARY;

EID: 56049099012     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20081068     Document Type: Article

References (24)

1. Al-Shawi, M. K. and Senior, A. E. (1993) Characterization of the adenosine triphosphatase activity of Chinese hamster P-glycoprotein. J. Biol. Chem. 268, 4197-4206
2. Tombline, G. and Senior, A. E. (2005) The occluded nucleotide conformation of p-glycoprotein. J. Bioenerg. Biomembr. 37, 497-500
3. Senior, A. E., al-Shawi, M. K. and Urbatsch, I. L. (1995) The catalytic cycle of P-glycoprotein. FEBS Lett. 377, 285-289
4. Tombline, G., Muharemagic, A., White, L. B. and Senior, A. E. (2005) Involvement of the occluded nucleotide conformation- of P-glycoprotein in the catalytic pathway. Biochemistry 44, 12879-12886
5. Sauna, Z. E., Smith, M. M., Muller, M., Kerr, K. M. and Ambudkar, S. V. (2001) The mechanism of action of multidrug-resistance-linked P-glycoprotein. J. Bioenerg. Biomembr. 33, 481-491
6. Van der Does, C., Presenti, C., Schulze, K., Dinkelaker, S. and Tampe, R. (2006) Kinetics of the ATP hydrolysis cycle of the nucleotide-binding domain of Mdl1 studied by a novel site-specific labeling technique. J. Biol. Chem. 281, 5694-5701
7. Tombline, G., Bartholomew, L. A., Urbatsch, I. L. and Senior, A. E. (2004) Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly. J. Biol. Chem. 279, 31212-31220
8. Sauna, Z. E., Nandigama, K. and Ambudkar, S. V. (2006) Exploiting reaction intermediates of the ATPase reaction to elucidate the mechanism of transport by P-glycoprotein (ABCB1). J. Biol. Chem. 281, 26501-26511
9. Martin, C., Higgins, C. F. and Callaghan, R. (2001) The vinblastine binding site adopts high- and low-affinity conformations during a transport cycle of P-glycoprotein. Biochemistry 40, 15733-15742
10. Aleksandrov, L., Aleksandrov, A. A., Chang, X. B. and Riordan, J. R. (2002) The first nucleotide binding domain of cystic fibrosis transmembrane conductance regulator is a site of stable nucleotide interaction, whereas the second is a site of rapid turnover. J. Biol. Chem. 277, 15419-15425
11. Basso, C., Vergani, P., Nairn, A. C. and Gadsby, D. C. (2003) Prolonged nonhydrolytic interaction of nucleotide with CFTR's NH2-terminal nucleotide binding domain and its role in channel gating. J. Gen. Physiol. 122, 333-348
12. Aleksandrov, L., Mengos, A., Chang, X., Aleksandrov, A. and Riordan, J. R. (2001) Differential interactions of nucleotides at the two nucleotide binding domains of the cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 276, 12918-12923
13. Cui, L., Aleksandrov, L., Chang, X. B., Hou, Y. X., He, L., Hegedus, T., Gentzsch, M., Aleksandrov, A., Balch, W. E. and Riordan, J. R. (2007) Domain interdependence in the biosynthetic assembly of CFTR. J. Mol. Biol. 365, 981-994
14. Kleizen, B., van Vlijmen, T., de Jonge, H. R. and Braakman, I. (2005) Folding of CFTR is predominantly cotranslational. Mol. Cell 20, 277-287
15. Lewis, H. A., Buchanan, S. G., Burley, S. K., Conners, K., Dickey, M., Dorwart, M., Fowler, R., Gao, X., Guggino, W. B., Hendrickson, W. A. et al. (2004) Structure of nucleotide-binding domain 1 of the cystic fibrosis transmembrane conductance regulator. EMBO J. 23, 282-293
16. Younger, J. M., Chen, L., Ren, H. Y., Rosser, M. F., Turnbull, E. L., Fan, C. Y., Patterson, C. and Cyr, D. M. (2006) Sequential quality-control checkpoints triage misfolded cystic fibrosis transmembrane conductance regulator. Cell 126, 571-582
17. Karpowich, N., Martsinkevich, O., Millen, L., Yuan, Y. R., Dai, P. L., MacVey, K., Thomas, P. J. and Hunt, J. F. (2001) Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter. Structure 9, 571-586
18. Jones, P. M. and George, A. M. (2000) Symmetry and structure in P-glycoprotein and ABC transporters: what goes around comes around. Eur. J. Biochem. 267, 5298-5305
19. Zaitseva, J., Jenewein, S., Jumpertz, T., Holland, I. B. and Schmitt, L. (2005) H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB. EMBO J. 24, 1901-1910
20. 2+-dependent steps rate limit opening and closing of a single CFTR Cl- channel. J. Gen. Physiol. 119, 545-559
21. Serohijos, A. W., Hegedus, T., Aleksandrov, A. A., He, L., Cui, L., Dokholyan, N. V. and Riordan, J. R. (2008) Phenylalanine-508 mediates a cytoplasmic-membrane domain contact in the CFTR 3D structure crucial to assembly and channel function. Proc. Natl. Acad. Sci. U.S.A. 105, 3256-3261
22. Li, C., Ramjeesingh, M. and Bear, C. E. (1996) Purified cystic fibrosis transmembrane conductance regulator (CFTR) does not function as an ATP channel. J. Biol. Chem. 271, 11623-11626
23. Mense, M., Vergani, P., White, D. M., Altberg, G., Nairn, A. C. and Gadsby, D. C. (2006) In vivo phosphorylation of CFTR promotes formation of a nucleotide-binding domain heterodimer. EMBO J. 25, 4728-4739
24. Powe, Jr, A. C., Al-Nakkash, L., Li, M. and Hwang, T. C. (2002) Mutation of Walker-A lysine 464 in cystic fibrosis transmembrane conductance regulator reveals functional interaction between its nucleotide-binding domains. J. Physiol. 539, 333-346