Integration analysis of MicroRNA and mRNA expression profiles in human peripheral blood lymphocytes cultured in modeled microgravity

BioMed Research International

Volumn 2014, Issue , 2014, Pages

  Girardi, C ^a   De Pitta C ^a   Casara, S ^a   Calura, E ^a   Romualdi, C ^a   Celotti, L ^a,b   Mognato, M ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b LABORATORI NAZIONALI DI LEGNARO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 17F; LYMPHOCYTE ANTIGEN; MESSENGER RNA; MICRORNA; MICRORNA 150 3P; MICRORNA 155 5P; MICRORNA 378 3P; MICRORNA 9 3P; MICRORNA 9 5P; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PROGRAMMED CELL DEATH 4 PROTEIN; UNCLASSIFIED DRUG; TRANSCRIPTOME;

APOPTOSIS; ARTICLE; BIOINFORMATICS; CELL PROLIFERATION; CELL VIABILITY ASSAY; CONTROLLED STUDY; CORRELATIONAL STUDY; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IMMUNE RESPONSE; INFLAMMATION; LYMPHOCYTE CULTURE; MICROGRAVITY; PERIPHERAL LYMPHOCYTE; REGULATORY MECHANISM; RNA ANALYSIS; SIGNAL TRANSDUCTION; STRESS; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENETICS; LYMPHOCYTE; METABOLISM; NORMAL HUMAN; PATHOLOGY; WEIGHTLESSNESS;

APOPTOSIS; CELL PROLIFERATION; GENE EXPRESSION REGULATION, NEOPLASTIC; HEALTHY VOLUNTEERS; HUMANS; LYMPHOCYTES; MICRORNAS; RNA, MESSENGER; SIGNAL TRANSDUCTION; TRANSCRIPTOME; WEIGHTLESSNESS;

EID: 84904102940     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/296747     Document Type: Article

References (98)

1. Fitts R. H., Riley D. R., Widrick J. J., Functional and structural adaptations of skeletal muscle to microgravity. Journal of Experimental Biology 2001 204 18 3201 3208 2-s2.0-0035745756 (Pubitemid 34861363)
2. Narici M., Kayser B., Barattini P., Cerretelli P., Effects of 17-day spaceflight on electrically evoked torque and cross-sectional area of the human triceps surae. European Journal of Applied Physiology 2003 90 3-4 275 282 2-s2.0-0345688651 10.1007/s00421-003-0955-7 (Pubitemid 37455687)
3. Trappe S., Costill D., Gallagher P., Creer A., Peters J. R., Evans H., Riley D. A., Fitts R. H., Exercise in space: human skeletal muscle after 6 months aboard the International Space Station. Journal of Applied Physiology 2009 106 4 1159 1168 2-s2.0-66149124537 10.1152/japplphysiol.91578.2008
4. Carlsson S. I. M., Bertilaccio M. T. S., Ballabio E., Maier J. A. M., Endothelial stress by gravitational unloading: effects on cell growth and cytoskeletal organization. Biochimica et Biophysica Acta 2003 1642 3 173 179 2-s2.0-0142169368 10.1016/j.bbamcr.2003.08.003 (Pubitemid 37315660)
5. Infanger M., Kossmehl P., Shakibaei M., Baatout S., Witzing A., Grosse J., Bauer J., Cogoli A., Faramarzi S., Derradji H., Neefs M., Paul M., Grimm D., Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor. Apoptosis 2006 11 5 749 764 2-s2.0-33745050928 10.1007/s10495-006-5697-7 (Pubitemid 43876844)
6. Baevsky R. M., Baranov V. M., Funtova I. I., Diedrich A., Pashenko A. V., Chernikova A. G., Drescher J., Jordan J., Tank J., Autonomic cardiovascular and respiratory control during prolonged spaceflights aboard the International Space Station. Journal of Applied Physiology 2007 103 1 156 161 2-s2.0-34447627941 10.1152/japplphysiol.00137.2007 (Pubitemid 47092287)
7. Sibonga J. D., Evans H. J., Sung H. G., Spector E. R., Lang T. F., Oganov V. S., Bakulin A. V., Shackelford L. C., LeBlanc A. D., Recovery of spaceflight-induced bone loss: bone mineral density after long-duration missions as fitted with an exponential function. Bone 2007 41 6 973 978 2-s2.0-36148938367 10.1016/j.bone.2007.08.022 (Pubitemid 350116745)
8. Keyak J. H., Koyama A. K., LeBlanc A., Lu Y., Lang T. F., Reduction in proximal femoral strength due to long-duration spaceflight. Bone 2009 44 3 449 453 2-s2.0-59649095748 10.1016/j.bone.2008.11.014
9. Ullrich O., Huber K., Lang K., Signal transduction in cells of the immune system in microgravity. Cell Communication and Signaling 2008 6, article 9 2-s2.0-66149170571 10.1186/1478-811X-6-9
10. Crucian B. E., Stowe R. P., Pierson D. L., Sams C. F., Immune system dysregulation following short- vs long-duration spaceflight. Aviation Space and Environmental Medicine 2008 79 9 835 843 2-s2.0-51149084801 10.3357/ASEM.2276. 2008
11. Sonnenfeld G., Butel J. S., Shearer W. T., Effects of the space flight environment on the immune system. Reviews on Environmental Health 2003 18 1 1 17 2-s2.0-0141432227 (Pubitemid 37236502)
12. Sonnenfeld G., Editorial: space flight modifies T cell activation-role of microgravity. Journal of Leukocyte Biology 2012 92 6 1125 1126
13. Semov A., Semova N., Lacelle C., Marcotte R., Petroulakis E., Proestou G., Wang E., Alterations in TNF- and IL-related gene expression in space-flown WI38 human fibroblasts. The FASEB Journal 2002 16 8 899 901 2-s2.0-0036615634
14. Chang T. T., Walther I., Li C.-F., Boonyaratanakornkit J., Galleri G., Meloni M. A., Pippia P., Cogoli A., Hughes-Fulford M., The Rel/NF- B pathway and transcription of immediate early genes in T cell activation are inhibited by microgravity. Journal of Leukocyte Biology 2012 92 6 1133 1145 2-s2.0-84870448663 10.1189/jlb.0312157
15. Lewis M. L., Cubano L. A., Zhao B., Dinh H. K., Pabalan J. G., Piepmeier E. H., Bowman P. D., cDNA microarray reveals altered cytoskeletal gene expression in space-flown leukemic T lymphocytes (Jurkat). The FASEB Journal 2001 15 10 1783 1785 2-s2.0-0035432020
16. Pardo S. J., Patel M. J., Sykes M. C., Platt M. O., Boyd N. L., Sorescu G. P., Xu M., Van Loon J. J. W. A., Wang M. D., Jo H., Simulated microgravity using the Random Positioning Machine inhibits differentiation and alters gene expression profiles of 2T3 preosteoblasts. American Journal of Physiology 2005 288 6 C1211 C1221 2-s2.0-21144445382 10.1152/ajpcell.00222.2004 (Pubitemid 40991058)
17. Monticone M., Liu Y., Pujic N., Cancedda R., Activation of nervous system development genes in bone marrow derived mesenchymal stem cells following spaceflight exposure. Journal of Cellular Biochemistry 2010 111 2 442 452 2-s2.0-77957269573 10.1002/jcb.22765
18. Grimm D., Bauer J., Kossmehl P., Shakibaei M., Schöberger J., Pickenhahn H., Schulze-Tanzil G., Vetter R., Eilles C., Paul M., Cogoli A., Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells. The FASEB Journal 2002 16 6 604 606 2-s2.0-0036548160
19. Maccarrone M., Battista N., Meloni M., Bari M., Galleri G., Pippia P., Cogoli A., Finazzi-Agrò A., Creating conditions similar to those that occur during exposure of cells to microgravity induces apoptosis in human lymphocytes by 5-lipoxygenase-mediated mitochondrial uncoupling and cytochrome c release. Journal of Leukocyte Biology 2003 73 4 472 481 2-s2.0-0037645143 10.1189/jlb.0602295 (Pubitemid 38040093)
20. Crawford-Young S. J., Effects of microgravity on cell cytoskeleton and embryogenesis. International Journal of Developmental Biology 2006 50 2-3 183 191 2-s2.0-33646358503 10.1387/ijdb.052077sc
21. Ward N. E., Pellis N. R., Risin S. A., Risin D., Gene expression alterations in activated human T-cells induced by modeled microgravity. Journal of Cellular Biochemistry 2006 99 4 1187 1202 2-s2.0-33750545620 10.1002/jcb.20988 (Pubitemid 44673974)
22. Clement J. Q., Lacy S. M., Wilson B. L., Gene expression profiling of human epidermal keratinocytes in simulated microgravity and recovery cultures. Genomics, Proteomics and Bioinformatics 2008 6 1 8 28 2-s2.0-45349101589 10.1016/S1672-0229(08)60017-0 (Pubitemid 351847758)
23. Kumari R., Singh K. P., DuMond J. W. Jr., Simulated microgravity decreases DNA repair capacity and induces DNA damage in human lymphocytes. Journal of Cellular Biochemistry 2009 107 4 723 731 2-s2.0-67650064636 10.1002/jcb.22171
24. Kang C.-Y., Zou L., Yuan M., Wang Y., Li T.-Z., Zhang Y., Wang J.-F., Li Y., Deng X.-W., Liu C.-T., Impact of simulated microgravity on microvascular endothelial cell apoptosis. European Journal of Applied Physiology 2011 111 9 2131 2138 2-s2.0-80053924623 10.1007/s00421-011-1844-0
25. Krützfeldt J., Poy M. N., Stoffel M., Strategies to determine the biological function of microRNAs. Nature Genetics 2006 38 1 S14 S19 2-s2.0-33745279604 10.1038/ng1799
26. Nickerson C. A., Ott C. M., Wilson J. W., Ramamurthy R., LeBlanc C. L., Höner zu Bentrup K., Hammond T., Pierson D. L., Low-shear modeled microgravity: a global environmental regulatory signal affecting bacterial gene expression, physiology, and pathogenesis. Journal of Microbiological Methods 2003 54 1 1 11 2-s2.0-0038218004 10.1016/S0167-7012(03)00018-6 (Pubitemid 36559595)
27. Arunasri K., Adil M., Venu Charan K., Suvro C., Himabindu Reddy S., Shivaji S., Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression. PLoS ONE 2013 8 3 2-s2.0-84874593121 10.1371/journal.pone. 0057860 e57860
28. Marcu O., Lera M. P., Sanchez M. E., Levic E., Higgins L. A., Shmygelska A., Fahlen T. F., Nichol H., Bhattacharya S., Innate immune responses of Drosophila melanogaster are altered by spaceflight. PLoS ONE 2011 6 1 2-s2.0-79251574946 10.1371/journal.pone.0015361 e15361
29. Honda Y., Higashibata A., Matsunaga Y., Yonezawa Y., Kawano T., Higashitani A., Kuriyama K., Shimazu T., Tanaka M., Szewczyk N. J., Ishioka N., Honda S., Genes down-regulated in spaceflight are involved in the control of longevity in Caenorhabditis elegans. Scientific Reports 2012 2, article 487 2-s2.0-84863800657 10.1038/srep00487
30. Manzano A. I., van Loon J. J. W. A., Christianen P. C. M., Gonzalez-Rubio J. M., Medina F. J., Herranz R., Gravitational and magnetic field variations synergize to cause subtle variations in the global transcriptional state of Arabidopsis in vitro callus cultures. BMC Genomics 2012 13 1, article 105 2-s2.0-84858606723 10.1186/1471-2164-13-105
31. Bartel D. P., MicroRNAs: target recognition and regulatory functions. Cell 2009 136 2 215 233 2-s2.0-58249088751 10.1016/j.cell.2009.01.002
32. Guo H., Ingolia N. T., Weissman J. S., Bartel D. P., Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 2010 466 7308 835 840 2-s2.0-77955644289 10.1038/nature09267
33. Huang V., Qin Y., Wang J., Wang X., Place R. F., Lin G., Lue T. F., Li L.-C., RNAa is conserved in mammalian cells. PLoS ONE 2010 5 1 2-s2.0-77749320938 10.1371/journal.pone.0008848 e8848
34. Frankel L. B., Christoffersen N. R., Jacobsen A., Lindow M., Krogh A., Lund A. H., Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells. The Journal of Biological Chemistry 2008 283 2 1026 1033 2-s2.0-38149042783 10.1074/jbc.M707224200
35. Poy M. N., Spranger M., Stoffel M., microRNAs and the regulation of glucose and lipid metabolism. Diabetes, Obesity and Metabolism 2007 9 2 67 73 2-s2.0-35148819250 10.1111/j.1463-1326.2007.00775.x (Pubitemid 47543128)
36. Stern-Ginossar N., Elefant N., Zimmermann A., Wolf D. G., Saleh N., Biton M., Horwitz E., Prokocimer Z., Prichard M., Hahn G., Goldman-Wohl D., Greenfield C., Yagel S., Hengel H., Altuvia Y., Margalit H., Mandelboim O., Host immune system gene targeting by a viral miRNA. Science 2007 317 5836 376 381 2-s2.0-34547132780 10.1126/science.1140956 (Pubitemid 47106378)
37. Marsit C. J., Eddy K., Kelsey K. T., MicroRNA responses to cellular stress. Cancer Research 2006 66 22 10843 10848 2-s2.0-33845312687 10.1158/0008-5472.CAN-06-1894 (Pubitemid 44876980)
38. Voorhoeve P. M., le Sage C., Schrier M., Gillis A. J. M., Stoop H., Nagel R., Liu Y.-P., van Duijse J., Drost J., Griekspoor A., Zlotorynski E., Yabuta N., De Vita G., Nojima H., Looijenga L. H. J., Agami R., A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 2006 124 6 1169 1181 2-s2.0-33646056975 10.1016/j.cell.2006.02.037
39. Wiemer E. A. C., The role of microRNAs in cancer: no small matter. European Journal of Cancer 2007 43 10 1529 1544 2-s2.0-34250201468 10.1016/j.ejca.2007.04.002 (Pubitemid 46901017)
40. Cho W. C. S., OncomiRs: the discovery and progress of microRNAs in cancers. Molecular Cancer 2007 6, article 60 2-s2.0-36649025655 10.1186/1476-4598-6-60 (Pubitemid 350193892)
41. Mi S., Lu J., Sun M., Li Z., Zhang H., Neilly M. B., Wang Y., Qian Z., Jin J., Zhang Y., Bohlander S. K., Le Beau M. M., Larson R. A., Golub T. R., Rowley J. D., Chen J., MicroRNA expression signatures accurately discriminate acute lymphoblastic leukemia from acute myeloid leukemia. Proceedings of the National Academy of Sciences of the United States of America 2007 104 50 19971 19976 2-s2.0-38049123402 10.1073/pnas.0709313104
42. Hammond S. M., MicroRNAs as tumor suppressors. Nature Genetics 2007 39 5 582 583 2-s2.0-34247555491 10.1038/ng0507-582 (Pubitemid 46676097)
43. Bisognin A., Sales G., Coppe A., Bortoluzzi S., Romualdi C., MAGIA2: from miRNA and genes expression data integrative analysis to microRNA-transcription factor mixed regulatory circuits (2012 update). Nucleic Acids Research 2012 40 1 W13 W21 2-s2.0-84864443057 10.1093/nar/gks460
44. Mognato M., Celotti L., Modeled microgravity affects cell survival and HPRT mutant frequency, but not the expression of DNA repair genes in human lymphocytes irradiated with ionising radiation. Mutation Research 2005 578 1-2 417 429 2-s2.0-25844492588 10.1016/j.mrfmmm.2005.06.011 (Pubitemid 41395342)
45. Unsworth B. R., Lelkes P. I., Growing tissues in microgravity. Nature Medicine 1998 4 8 901 907 2-s2.0-0031904597 10.1038/nm0898-901 (Pubitemid 28364028)
46. Hou S.-M., Van Dam F. J., De Zwart F., Warnock C., Mognato M., Turner J., Podlutskaja N., Podlutsky A., Becker R., Barnett Y., Barnett C. R., Celotti L., Davies M., Hüttner E., Lambert B., Tates A. D., Validation of the human T-lymphocyte cloning assay-ring test report from the EU concerted action on HPRT mutation (EUCAHM). Mutation Research 1999 431 2 211 221 2-s2.0-0033381999 10.1016/S0027-5107(99)00164-5 (Pubitemid 30017792)
47. Girardi C., de Pittà C., Casara S., Sales G., Lanfranchi G., Celotti L., Mognato M., Analysis of miRNA and mRNA expression profiles highlights alterations in ionizing radiation response of human lymphocytes under modeled microgravity. PLoS ONE 2012 7 2 2-s2.0-84856786498 10.1371/journal. pone.0031293 e31293
48. Wang H., Ach R. A., Curry B. O., Direct and sensitive miRNA profiling from low-input total RNA. RNA 2007 13 1 151 159 2-s2.0-33845876339 10.1261/rna.234507 (Pubitemid 46026189)
49. Bolstad B. M., Irizarry R. A., Åstrand M., Speed T. P., A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics 2003 19 2 185 193 2-s2.0-0037316303 10.1093/bioinformatics/19.2.185 (Pubitemid 36181903)
50. Tusher V. G., Tibshirani R., Chu G., Diagnosis of multiple cancer types by shrunken centroids of gene expression. Proceedings of the National Academy of Sciences of the United States of America 2001 98 5116 5121
51. Sales G., Calura E., Martini P., Romualdi C., Graphite web: web tool for gene set analysis exploiting pathway topology. Nucleic Acids Research 2013 41 89 97
52. Xin F., Li M., Balch C., Thomson M., Fan M., Liu Y., Hammond S. M., Kim S., Nephew K. P., Computational analysis of microRNA profiles and their target genes suggests significant involvement in breast cancer antiestrogen resistance. Bioinformatics 2009 25 4 430 434 2-s2.0-60149084355 10.1093/bioinformatics/ btn646
53. Wang H., Li W.-H., Increasing MicroRNA target prediction confidence by the relative R2 method. Journal of Theoretical Biology 2009 259 4 793 798 2-s2.0-67650725491 10.1016/j.jtbi.2009.05.007
54. Huang D. W., Sherman B. T., Lempicki R. A., Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols 2009 4 1 44 57 2-s2.0-61449172037 10.1038/nprot.2008.211
55. -ΔΔCT method. Methods 2001 25 4 402 408 2-s2.0-0035710746 10.1006/meth.2001.1262 (Pubitemid 34164012)
56. Albertini R. J., Castle K. L., Borcherding W. R., T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood. Proceedings of the National Academy of Sciences of the United States of America 1982 79 21 I 6617 6621 2-s2.0-0342412296 (Pubitemid 13220226)
57. Mognato M., Girardi C., Fabris S., Celotti L., DNA repair in modeled microgravity: double strand break rejoining activity in human lymphocytes irradiated with γ -rays. Mutation Research 2009 663 1-2 32 39 2-s2.0-63549120722 10.1016/j.mrfmmm.2009.01.002
58. Canova S., Fiorasi F., Mognato M., Grifalconi M., Reddi E., Russo A., Celotti L., "Modeled microgravity" affects cell response to ionizing radiation and increases genomic damage. Radiation Research 2005 163 2 191 199 2-s2.0-12844266749 10.1667/RR3304 (Pubitemid 40170769)
59. Lewis B. P., Burge C. B., Bartel D. P., Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 2005 120 1 15 20 2-s2.0-11844278458 10.1016/j.cell.2004.12.035 (Pubitemid 40094598)
60. Cline M. S., Smoot M., Cerami E., Kuchinsky A., Landys N., Workman C., Christmas R., Avila-Campilo I., Creech M., Gross B., Hanspers K., Isserlin R., Kelley R., Killcoyne S., Lotia S., Maere S., Morris J., Ono K., Pavlovic V., Pico A. R., Vailaya A., Wang P.-L., Adler A., Conklin B. R., Hood L., Kuiper M., Sander C., Schmulevich I., Schwikowski B., Warner G. J., Ideker T., Bader G. D., Integration of biological networks and gene expression data using Cytoscape. Nature Protocols 2007 2 10 2366 2382 2-s2.0-38449101120 10.1038/nprot.2007.324
61. Censi F., Giuliani A., Bartolini P., Calcagnini G., A multiscale graph theoretical approach to gene regulation networks: a case study in atrial fibrillation. IEEE Transactions on Biomedical Engineering 2011 58 10 2943 2946 2-s2.0-80053143005 10.1109/TBME.2011.2150747
62. Chen P., Price C., Li Z., Li Y., Cao D., Wiley A., He C., Gurbuxani S., Kunjamma R. B., Huang H., Jiang X., Arnovitz S., Xu M., Hong G.-M., Elkahloun A. G., Neilly M. B., Wunderlich M., Larson R. A., Le Beau M. M., Mulloy J. C., Liu P. P., Rowley J. D., Chen J., miR-9 is an essential oncogenic microRNA specifically overexpressed in mixed lineage leukemia-rearranged leukemia. Proceedings of the National Academy of Sciences of the United States of America 2013 110 28 11511 11516 2-s2.0-84879932160 10.1073/pnas.1310144110
63. Pignot G., Cizeron-Clairac G., Vacher S., Susini A., Tozlu S., Vieillefond A., Zerbib M., Lidereau R., Debre B., Amsellem-Ouazana D., Bieche I., MicroRNA expression profile in a large series of bladder tumors: identification of a 3-miRNA signature associated with aggressiveness of muscle-invasive bladder cancer. International Journal of Cancer 2013 132 11 2479 2491 2-s2.0-84875614996 10.1002/ijc.27949
64. Namløs H. M., Meza-Zepeda L. A., Barøy T., Østensen I. H. G., Kresse S. H., Kuijjer M. L., Serra M., Bürger H., Cleton-Jansen A.-M., Myklebost O., Modulation of the osteosarcoma expression phenotype by microRNAs. PLoS ONE 2012 7 10 2-s2.0-84868110139 10.1371/journal.pone.0048086 e48086
65. Eis P. S., Tam W., Sun L., Chadburn A., Li Z., Gomez M. F., Lund E., Dahlberg J. E., Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proceedings of the National Academy of Sciences of the United States of America 2005 102 10 3627 3632 2-s2.0-14844354250 10.1073/pnas.0500613102 (Pubitemid 40354662)
66. Pan Y., Meng M., Zhang G., Han H., Zhou Q., Oncogenic microRNAs in the genesis of leukemia and lymphoma. Current Pharmaceutical Design 2014 10.2174/1381612820666140128211724
67. Iorio M. V., Ferracin M., Liu C.-G., Veronese A., Spizzo R., Sabbioni S., Magri E., Pedriali M., Fabbri M., Campiglio M., Ménard S., Palazzo J. P., Rosenberg A., Musiani P., Volinia S., Nenci I., Calin G. A., Querzoli P., Negrini M., Croce C. M., MicroRNA gene expression deregulation in human breast cancer. Cancer Research 2005 65 16 7065 7070 2-s2.0-23844555119 10.1158/0008-5472.CAN-05-1783 (Pubitemid 41161233)
68. Lu Z., Ye Y., Jiao D., Qiao J., Cui S., Liu Z., MiR-155 and miR-31 are differentially expressed in breast cancer patients and are correlated with the estrogen receptor and progesterone receptor status. Oncology Letters 2012 4 5 1027 1032 2-s2.0-84866141386 10.3892/ol.2012.841
69. Gao F., Chang J., Wang H., Zhang G., Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients. Oncology Reports 2014 31 1 351 357
70. Higgs G., Slack F., The multiple roles of microRNA-155 in oncogenesis. Journal of Clinical Bioinformatics 2013 3 1 17
71. Fayyad-Kazan H., Bitar N., Najar M., Lewalle P., Fayyad-Kazan M., Badran R., Hamade E., Daher A., Hussein N., ELDirani R., Berri F., Vanhamme L., Burny A., Martiat P., Rouas R., Badran B., Circulating miR-150 and miR-342 in plasma are novel potential biomarkers for acute myeloid leukemia. Journal of Translational Medicine 2013 11 1, article 31 2-s2.0-84874191746 10.1186/1479-5876-11-31
72. Yanlei M., Zhang P., Wang F., Zhang H., Yang J., Peng J., Liu W., Qin H., miR-150 as a potential biomarker associated with prognosis and therapeutic outcome in colorectal cancer. Gut 2012 61 10 1447 1453 2-s2.0-84866098272 10.1136/gutjnl-2011-301122
73. Jacob N. K., Cooley J. V., Yee T. N., Jacob J., Alder H., Wickramasinghe P., Maclean K. H., Chakravarti A., Identification of Sensitive Serum microRNA Biomarkers for Radiation Biodosimetry. PLoS ONE 2013 8 2 2-s2.0-84874345725 10.1371/journal.pone.0057603 e57603
74. Zhang G. J., Zhou H., Xiao H. X., Li Y., Zhou T., MiR-378 is an independent prognostic factor and inhibits cell growth and invasion in colorectal cancer. BMC Cancer 2014 14 1 109
75. Sand M., Skrygan M., Georgas D., Sand D., Hahn S. A., Gambichler T., Altmeyer P., Bechara F. G., Microarray analysis of microRNA expression in cutaneous squamous cell carcinoma. Journal of Dermatological Science 2012 68 3 119 126 2-s2.0-84868592288 10.1016/j.jdermsci.2012.09.004
76. Hauser S., Wulfken L. M., Holdenrieder S., Moritz R., Ohlmann C.-H., Jung V., Becker F., Herrmann E., Walgenbach-Brünagel G., von Ruecker A., Müller S. C., Ellinger J., Analysis of serum microRNAs (miR-26a-2- miR-191, miR-337-3p and miR-378) as potential biomarkers in renal cell carcinoma. Cancer Epidemiology 2012 36 4 391 394 2-s2.0-84863465388 10.1016/j.canep.2012.04.001
77. Mangala L. S., Zhang Y., He Z., Emami K., Ramesh G. T., Story M., Rohde L. H., Wu H., Effects of simulated microgravity on expression profile of microRNA in human lymphoblastoid cells. The Journal of Biological Chemistry 2011 286 37 32483 32490 2-s2.0-80052783075 10.1074/jbc.M111.267765
78. Alexiou P., Maragkakis M., Papadopoulos G. L., Reczko M., Hatzigeorgiou A. G., Lost in translation: an assessment and perspective for computational microrna target identification. Bioinformatics 2009 25 23 3049 3055 2-s2.0-74049088856 10.1093/bioinformatics/btp565
79. Nunez-Iglesias J., Liu C.-C., Morgan T. E., Finch C. E., Zhou X. J., Joint genome-wide profiling of miRNA and mRNA expression in Alzheimer's disease cortex reveals altered miRNA regulation. PLoS ONE 2010 5 2 2-s2.0-77953312769 10.1371/journal.pone.0008898 e8898
80. Ma L., Huang Y., Zhu W., Zhou S., Zhou J., Zeng F., Liu X., Zhang Y., Yu J., An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers. PLoS ONE 2011 6 10 2-s2.0-80055026432 10.1371/journal.pone.0026502 e26502
81. Engelmann J. C., Spang R., A least angle regression model for the prediction of canonical and non-canonical miRNA-mRNA interactions. PLoS ONE 2012 7 7 2-s2.0-84863995800 10.1371/journal.pone.0040634 e40634
82. Bossel Ben-Moshe N., Avraham R., Kedmi M., Zeisel A., Yitzhaky A., Yarden Y., Domany E., Context-specific microRNA analysis: identification of functional microRNAs and their mRNA targets. Nucleic Acids Research 2012 40 21 10614 10627 2-s2.0-84870612323 10.1093/nar/gks841
83. Artmann S., Jung K., Bleckmann A., Beißbarth T., Detection of simultaneous group effects in microRNA expression and related target gene sets. PLoS ONE 2012 7 6 2-s2.0-84862562497 10.1371/journal.pone.0038365 e38365
84. Germain R. N., MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation. Cell 1994 76 2 287 299 2-s2.0-0028038426 10.1016/0092-8674(94)90336-0
85. Konstantinova I. V., Antropova E. N., Legenkov V. I., Zazhirey V. D., Study of the reactivity of blood lymphoid cells in crew members of Soyuz 6, 7 and 8 before and after space flight Kosmicheskaia Biologiia i Meditsina 1973 7 6 35 40 2-s2.0-0015817944
86. Cogoli A., Tschopp A., Lymphocyte reactivity during spaceflight. Immunology Today 1985 6 1 1 4 2-s2.0-0022008097
87. Guéguinou N., Huin-Schohn C., Bascove M., Bueb J.-L., Tschirhart E., Legrand-Frossi C., Frippiat J.-P., Could spaceflight-associated immune system weakening preclude the expansion of human presence beyond Earth's orbit? Journal of Leukocyte Biology 2009 86 5 1027 1038 2-s2.0-70350741504 10.1189/jlb.0309167
88. Nunez Y. O., Truitt J. M., Gorini G., Ponomareva O. N., Blednov Y. A., Harris R. A., Mayfield R. D., Positively correlated miRNA-mRNA regulatory networks in mouse frontal cortex during early stages of alcohol dependence. BMC Genomics 2013 14 725
89. Sullivan R. P., Fogel L. A., Leong J. W., MicroRNA-155 tunes both the threshold and extent of NK cell activation via targeting of multiple signaling pathways. The Journal of Immunology 2013 191 12 5904 5913 10.4049/jimmunol. 1301950
90. Gao F., Zhao Z.-L., Zhao W.-T., Fan Q.-R., Wang S.-C., Li J., Zhang Y.-Q., Shi J.-W., Lin X.-L., Yang S., Xie R.-Y., Liu W., Zhang T.-T., Sun Y.-L., Xu K., Yao K.-T., Xiao D., MiR-9 modulates the expression of interferon-regulated genes and MHC class I molecules in human nasopharyngeal carcinoma cells. Biochemical and Biophysical Research Communications 2013 431 3 610 616 2-s2.0-84873711276 10.1016/j.bbrc.2012.12.097
91. Bazzoni F., Rossato M., Fabbri M., Gaudiosi D., Mirolo M., Mori L., Tamassia N., Mantovani A., Cassatella M. A., Locati M., Induction and regulatory function of miR-9 in human monocytes and neutrophils exposed to proinflammatory signals. Proceedings of the National Academy of Sciences of the United States of America 2009 106 13 5282 5287 2-s2.0-65249131826 10.1073/pnas.0810909106
92. + T cells by repressing Blimp-1. European Journal of Immunology 2012 42 8 2100 2108 2-s2.0-84864754110 10.1002/eji.201142203
93. Singh M., Del carpio-Cano F., Belcher J. Y., Crawford K., Frara N., Owen T. A., Popoff S. N., Safadi F. F., Functional roles of osteoactivin in normal and disease processes. Critical Reviews in Eukaryotic Gene Expression 2010 20 4 341 357 2-s2.0-79955027278
94. Cogoli-Greuter M., Meloni M. A., Sciola L., Spano A., Pippia P., Monaco G., Cogoli A., Movements and interactions of leukocytes in microgravity. Journal of Biotechnology 1996 47 2-3 279 287 2-s2.0-0030603759 10.1016/0168-1656(96) 01380-6 (Pubitemid 26276444)
95. Walther I., Pippia P., Meloni M. A., Turrini F., Mannu F., Cogoli A., Simulated microgravity inhibits the genetic expression of interleukin-2 and its receptor in mitogen-activated T lymphocytes. FEBS Letters 1998 436 1 115 118 2-s2.0-0032566605 10.1016/S0014-5793(98)01107-7 (Pubitemid 28454617)
96. Ma X., Pietsch J., Wehland M., Differential gene expression profile and altered cytokine secretion of thyroid cancer cells in space. The FASEB Journal 2014 28 2 813 835
97. Chang D., Xu H., Guo Y., Jiang X., Liu Y., Li K., Pan C., Yuan M., Wang J., Li T., Liu C., Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line. In Vitro Cellular and Developmental Biology-Animal 2013 49 3 170 177 2-s2.0-84877824274 10.1007/s11626-013-9581-9
98. Hong S., Li X., Zhao Y., Yang Q., Kong B., 53BP1 suppresses tumor growth and promotes susceptibility to apoptosis of ovarian cancer cells through modulation of the Akt pathway. Oncology Reports 2012 27 4 1251 1257 2-s2.0-84863023461 10.3892/or.2012.1641