Over-expression of native Saccharomyces cerevisiae exocytic SNARE genes increased heterologous cellulase secretion

Applied Microbiology and Biotechnology

Volumn 98, Issue 12, 2014, Pages 5567-5578

  Van Zyl, John Henry D ^a   Den Haan, Riaan ^a   Van Zyl, Willem H ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Cellulases; Improvement; Secretion; SNAREs; Yeast

Indexed keywords

CELL MEMBRANES; PHYSIOLOGY; PROTEINS; YEAST;

CELLULASES; ENGINEERING COMPONENTS; IMPROVEMENT; INTRACELLULAR MEMBRANES; PROTEIN TRAFFICKING; SECRETION; SNARES; TALAROMYCES EMERSONII;

GENE EXPRESSION;

CELLULASE; FUNGAL PROTEIN; SNARE PROTEIN; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

CONCENTRATION (COMPOSITION); ENZYME ACTIVITY; GENE EXPRESSION; OSMOSIS; PLASMA; PROTEIN; SECRETION; SODIUM CHLORIDE; YEAST;

ALCOHOL TOLERANCE; ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; FUNGUS; GENE DOSAGE; HETEROLOGOUS EXPRESSION; INTRACELLULAR MEMBRANE; LIMIT OF QUANTITATION; NONHUMAN; OSMOTIC STRESS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SUBUNIT; QUANTITATIVE ANALYSIS; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCOPSIS FIBULIGERA; SECRETORY PATHWAY; TALAROMYCES EMERSONII; BIOSYNTHESIS; ENZYMOLOGY; EUROTIALES; GENE EXPRESSION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES; SECRETION (PROCESS);

SACCHAROMYCES CEREVISIAE; SACCHAROMYCOPSIS FIBULIGERA; TALAROMYCES EMERSONII;

CELLULASE; EUROTIALES; GENE EXPRESSION; GENES, REPORTER; METABOLIC ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SNARE PROTEINS;

EID: 84903821579     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5647-1     Document Type: Article

References (46)

1. Burri L, Lithgow T (2004) A complete set of SNAREs in yeast. Traffic 5:45-52 (Pubitemid 38159403)
2. Byrne KP, Wolfe KH (2005) The yeast gene order browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Gen Res 15:1456-1461 (Pubitemid 41400914)
3. Çelik E, Çalik P (2012) Production of recombinant proteins by yeast cells. Biotechnol Adv 30:1108-1118
4. Demain A, Vaishnav P (2009) Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 27:297-306
5. Den Haan R, Rose S, Lynd L, Van Zyl WH (2007) Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng 9:87-94 (Pubitemid 44960875)
6. Den Haan R, Kroukamp H, Van Zyl JHDV, Van Zyl WH (2013) Cellobiohydrolase secretion by yeast: current state and prospects for improvement. Process Biochem 48:1-12
7. Di Sansebastiano G (2013) Defining new SNARE functions: the i-SNARE. Front Plant Sci 4:99
8. Galdieri L, Mehrotra S, Yu S, Vancura A (2010) Transcriptional regulation in yeast during diauxic shift and stationary phase. OMICS 14:629-638
9. Gasser B, Sauer M, Maurer M, Stadlmayr G, Mattanovich D (2007) Transcriptomics-based identification of novel factors enhancing heterologous protein secretion in yeasts. Appl Environ Microbiol 73:6499-6507 (Pubitemid 47623708)
10. Harford N, Cabezon T, Colau B, Delisse AM, Rutgers T, De Wilde M (1987) Construction and characterization of a Saccharomyces cerevisiae strain (RIT4376) expressing hepatitis B surface antigen. Postgrad Med J 63(2):65-70 (Pubitemid 17094403)
11. Hill J, Donald KA, Griffiths DE, Donald G (1991) DMSO-enhanced whole cell yeast transformation. Nucleic Acids Res 19(20):5791
12. Hoffman CS, Winston F (1987) A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57:267-272 (Pubitemid 18007116)
13. Hou J, Tyo K, Liu Z, Petranovic D, Nielsen J (2012) Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae. Metab Eng 14:120-127
14. Idiris A, Tohda H, Kumagai H, Takegawa K (2010) Engineering of protein secretion in yeast: strategies and impact on protein production. Appl Microbiol Biotechnol 86:403-417
15. Ilmén M, Den Haan R, Brevnova E, McBride J, Wiswall E, Froehlich A, Koivula A, Voutilainen SP, Siika-aho M, La Grange DC, Thorngren N, Ahlgren S, Mellon M, Deleault K, Rajgarhia V, Van Zyl WH, Pentillä M (2011) High level secretion of cellobiohydrolases by Saccharomyces cerevisiae. Biotechnol Biofuels 4:30
16. Jahn R, Scheller RH (2006) SNAREs - engines for membrane fusion. Nat Rev Mol Cell Bio 7:631-643 (Pubitemid 44268209)
17. Jäntti J, Aalto M, Oyen M, Sundqvist L, Keränen S, Ronne H (2002) Characterization of temperature-sensitive mutations in the yeast syntaxin 1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J Cell Sci 115:409-420 (Pubitemid 34145189)
18. Jüschke C, Wächter A, Schwappach B, Seedorf M (2005) SEC18/NSF-independent, protein sorting pathway from the yeast cortical ER to the plasma membrane. J Cell Biol 169(4):613-622 (Pubitemid 41002854)
19. Kloepper TH, Kienle CN, Fasshauer D (2008) SNAREing the basis of multicellularity: consequences of protein family expansion during evolution. Mol Biol Evol 25(9):2055-2068
20. Krantz M, Ahmadpour D, Ottosson L, Warringer J, Waltermann C, Nordlander B, Klipp E, Blomberg A, Hohmann S, Kitano H (2009) Robustness and fragility in the yeast high osmolarity glycerol (HOG) signal transduction pathway. Mol Syst Biol 5:281
21. Kroukamp H, Den Haan R, Van Wyk N, Van Zyl WH (2013) Overexpression of native PSE1 and SOD1 in Saccharomyces cerevisiae improved heterologous cellulase secretion. Appl Energy 102:150-156
22. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680-685
23. La Grange DC, Pretorius IS, Claeyssens M, Van Zyl WH (2001) Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei xylanase II (Xyn2) genes. Appl Environ Microbiol 67(12), 5512v5519
24. Lee FW, Da Silva NA (1997) Sequential delta integration for the regulated insertion of cloned genes in Saccharomyces cerevisiae. Biotechnol Progr 13(4):368-373 (Pubitemid 27335823)
25. Lynd L, Van Zyl W, McBride J, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16:577-583 (Pubitemid 41393839)
26. Malsam J, Kreye S, Söllner T (2008) Membrane fusion: SNAREs and regulation. CMLS Cell Mol Life S 65:2814-2832
27. McBride JE, Zietsman JJ, Van Zyl WH, Lynd LR (2005) Utilization of cellobiose by recombinant β-glucosidase-expressing strains of Saccharomyces cerevisiae: characterization and evaluation of the sufficiency of expression. Enzyme Microb Technol 37:93-101 (Pubitemid 40615648)
28. McBride JEE, Deleault KM, Lynd LR, Pronk JT (2008) Recombinant yeast strains expressing tethered cellulase enzymes. Patent PCT/US2007/085390
29. Nigam JM, Ireland RS, Margaritus A, Lachance MA (1985) Isolation and screening of yeasts that ferment D-xylose directly to ethanol. Appl Environ Microbiol 50(6):1486-1489 (Pubitemid 16213366)
30. Ostergaard S, Olsson L, Nielsen J (2000) Metabolic engineering of Saccharomyces cerevisiae. Microbiol Mol Biol R 64(1):34-50 (Pubitemid 30143839)
31. Protopopov V, Govindan B, Novick P, Gerst JE (1993) Homologues of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae. Cell 74(5):855-861 (Pubitemid 23270605)
32. Rader RA (2007) Biopharmaceutical products in the US and European markets, 6th edn. BioPlan Associates, Rockville
33. Romanos MA, Scorer CA, Clarke JJ (1992) Foreign gene expression in yeast: a review. Yeast 8:423-488
34. Romanos M (1995) Advances in the use of Pichia pastoris for high-level gene expression. Curr Opin Biotechnol 6:527-533
35. Ruohonen L, Toikkanen J, Tieaho V, Outola M, Soderlund H, Keranen S (1997) Enhancement of protein secretion in Saccharomyces cerevisiae by overproduction of Sso protein, a late-acting component of the secretory pathway. Yeast 13(4):337-351 (Pubitemid 27192244)
36. Sambrook J, Russel DB (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
37. Schmidt FR (2004) Recombinant expression systems in the pharmaceutical industry. Appl Microbiol Biotechnol 65:363-372
38. Schröder M (2008) Engineering eukaryotic protein factories. Biotechnol Lett 30:187-196
39. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, Reinisch K, McNew JA, Novick P (2013) The synaptobrevin homologue Snc2p recruits the exocyst to the secretory vesicle by binding to Sec6p. J Cell Biol 202(3):509-526
40. Stephen JD, Mabee WE, Saddler JN (2012) Will second-generation ethanol be able to compete with first-generation ethanol? Opportunities for cost reduction. Biofuels Bioprod Bioref 6:159-176
41. Teste M, Duquenne M, François JM, Parrou J (2009) Validation of reference genes for quantitative expression analysis by real-time RT-PCR in Saccharomyces cerevisiae. BMC Mol Biol 10:99
42. Van Rensburg E, Den Haan R, Smith J, Van Zyl WH, Görgens JF (2012) The metabolic burden of cellulase expression by recombinant Saccharomyces cerevisiae Y294 in aerobic batch culture. Appl Microbiol Biotechnol 96(1):197-209
43. Van Rooyen R, Hahn-Hägerdal B, La Grange DC, Van Zyl WH (2005) Construction of cellobiose-growing Saccharomyces cerevisiae strains. J Biotechnol 120:284-295 (Pubitemid 41540385)
44. Weinberger A, Kamena F, Kama R, Spang A, Gerst J (2005) Control of Golgimorphology and function by Sed5 t-SNARE phosphorylation. Mol Biol Cell 16:4918-4930 (Pubitemid 41416470)
45. Xu L, Shen Y, Hou J, Peng B, Tang H, Bao X (2013) Secretory pathway engineering enhances secretion of cellobiohydrolase I from Trichoderma reesei in Saccharomyces cerevisiae. J Biosci Bioeng. doi:10.1016/j.jbiosc.2013.06.017
46. YoshikawaK, Tanaka T, Ida Y, Furusawa C, Hirasawa T, Shimizu H (2011) Comprehensive phenotypic analysis of single-gene deletion and overexpression strains of Saccharomyces cerevisiae. Yeast 28:349-361