Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers

Enzyme and Microbial Technology

Volumn 60, Issue , 2014, Pages 1-8

  dos Santos, Jose C S ^a,b   Garcia Galan, Cristina ^a   Rodrigues, Rafael C ^c   de Sant' Ana, Hosiberto Batista ^b   Gonçalves, Luciana R B ^b   Fernandez Lafuente, Roberto ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

^c FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Enzyme hyperactivation; Enzyme stabilization; Interfacial activation; Lecitase Ultra; Polyethylenimine; Sulfate dextran

Indexed keywords

ENZYME STABILIZATION; HYPERACTIVATION; INTERFACIAL ACTIVATION; LECITASE; POLYETHYLENIMINES;

ENZYMES; ISOMERS; POLYMERS; STABILIZATION; SUBSTRATES;

PLASTIC COATINGS;

ACETIC ACID DERIVATIVE; ACETONITRILE; DEXTRAN SULFATE; IMMOBILIZED ENZYME; PARAOXON; POLYETHYLENEIMINE; BIOMATERIAL; CYANOGEN BROMIDE; HYBRID PROTEIN; PHOSPHOLIPASE A1; SEPHAROSE;

ARTICLE; COVALENT BOND; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME STABILITY; HALF LIFE TIME; HYDROLYSIS; PH; BIOTECHNOLOGY; CATALYSIS; CHEMISTRY; METABOLISM;

BIOTECHNOLOGY; CATALYSIS; COATED MATERIALS, BIOCOMPATIBLE; CYANOGEN BROMIDE; DEXTRAN SULFATE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; PHOSPHOLIPASES A1; POLYETHYLENEIMINE; RECOMBINANT FUSION PROTEINS; SEPHAROSE;

EID: 84903365325     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.03.001     Document Type: Article

References (44)

1. Havn S.S., Ipsen R., Nielsen P.M., Lilbæk H.M. Improving the foaming properties and heat stability of whey protein concentrates by phospholipase treatment. Milchwissenschaft 2006, 61:188-191.
2. Yang J.G., Wang Y.H., Yang B., Mainda G., Guo Y. Degumming of vegetable oil by a new microbial lipase. Food Technol Biotechnol 2006, 44:101-104.
3. Na A., Eriksson C., Eriksson S.G., Österberg E., Holmberg K. Synthesis of phosphatidylcholine with (n-3) fatty acids by phospholipase A 2 in microemulsion. J Am Oil Chem Soc 1990, 67:766-770.
4. Devos M., Poisson L., Ergan F., Pencreac'h G. Enzymatic hydrolysis of phospholipids from Isochrysis galbana for docosahexaenoic acid enrichment. Enzyme Microb Technol 2006, 39:548-554.
5. Yamamoto Y., Hosokawa M., Miyashita K. Production of phosphatidylcholine containing conjugated linoleic acid mediated by phospholipase A2. J Mol Catal B: Enzym 2006, 41:92-96.
6. De Maria L., Vind J., Oxenbøll K.M., Svendsen A., Patkar S. Phospholipases and their industrial applications. Appl Microbiol Biotechnol 2007, 74:290-300.
7. ® Ultra. J Mol Catal B: Enzym 2007, 47:99-104.
8. Yang B., Wang Y.H., Yang J.G. Optimization of enzymatic degumming process for rapeseed oil. J Am Oil Chem Soc 2006, 83:653-658.
9. Kim I.H., Garcia H.S., Hill C.G. Phospholipase A1-catalyzed synthesis of phospholipids enriched in n-3 polyunsaturated fatty acid residues. Enzyme Microb Technol 2007, 40:1130-1135.
10. Gonçalves K.M., Sutili F.K., Júnior I.I., Flores M.C., Soter De Mariz L., Miranda E., et al. A comprehensive study on the activity and deactivation of immobilized lecitase ultra in esterifications of food waste streams to monoacylglycerols. ChemSusChem 2013, 6:872-879.
11. ® Ultra onto a novel polystyrene DA-201 resin: characterization and biochemical properties. Appl Biochem Biotechnol 2012, 168:1108-1120.
12. Liu N., Wang Y., Zhao Q., Cui C., Fu M., Zhao M. Immobilisation of Lecitase® Ultra for production of diacylglycerols by glycerolysis of soybean oil. Food Chem 2012, 134:301-307.
13. Liu N., Wang Y., Zhao Q., Zhang Q., Zhao M. Fast synthesis of 1 3-DAG by Lecitase® Ultra-catalyzed esterification in solvent-free system. Eur J Lipid Sci Technol 2011, 113:973-979.
14. Mishra M.K., Harini M., Kumaraguru T., Lakshmi Prasanna T., Fadnavis N.W. A porous vessel bioreactor for gel entrapped biocatalysts: kinetic resolution of trans-methyl (4-methoxyphenyl)glycidate by Lecitase® Ultra in gelatin organogel (Gelozyme). J Mol Catal B: Enzym 2011, 71:56-62.
15. Wang Y., Zhao M., Song K., Wang L., Tang S., Riley W.W. Partial hydrolysis of soybean oil by phospholipase A1 (Lecitase Ultra). Food Chem 2010, 121:1066-1072.
16. Wang L., Wang Y., Hu C., Cao Q., Yang X., Zhao M. Preparation of diacylglycerol-enriched oil from free fatty acids using lecitase ultra-catalyzed esterification. J Am Oil Chem Soc 2011, 88:1557-1565.
17. Rodrigues R.C., Ortiz C., Berenguer-Murcia A., Torres R., Fernandez-Lafuente R. Modifying enzyme activity and selectivity by immobilization. Chem Soc Rev 2013, 42:6290-6307.
18. Miled N., Beisson F., De Caro J., De Caro A., Arondel V., Verger R. Interfacial catalysis by lipases. J Mol Catal B: Enzym 2001, 11:165-171.
19. Derewenda U., Swenson L., Green R., Wei Y., Yamaguchi S., Joerger R., et al. Current progress in crystallographic studies of new lipases from filamentous fungi. Protein Eng 1994, 7:551-557.
20. ® Ultra as regioselective biocatalyst in the hydrolysis of fully protected carbohydrates. Strong modulation by using different immobilization protocols. J Mol Catal B: Enzym 2008, 51:110-117.
21. ®. Effect of the immobilization protocol on its catalytic properties. Enzyme Microb Technol 2008, 43:531-536.
22. Barbosa O., Ruiz M., Ortiz C., Fernández M., Torres R., Fernandez-Lafuente R. Modulation of the properties of immobilized CALB by chemical modification with 2,3,4-trinitrobenzenesulfonate or ethylendiamine. Advantages of using adsorbed lipases on hydrophobic supports. Process Biochem 2012, 47:867-876.
23. Rodrigues R.C., Berenguer-Murcia Á., Fernandez-Lafuente R. Coupling chemical modification and immobilization to improve the catalytic performance of enzymes. Adv Synth Catal 2011, 353:2216-2238.
24. Garcia-Galan C.J.C.S., Santos d., Barbosa O., Torres R., Pereira E.B., Corberan V.C., et al. Tuning of Lecitase features via solid-phase chemical modification: effect of the immobilization protocol. Process Biochem 2014, 49(4):604-616. 10.1016/j.procbio.01. 028.
25. Fernández-Lorente G., Betancor L., Carrascosa A.V., Palomo J.M., Guisan J.M. Modulation of the selectivity of immobilized lipases by chemical and physical modifications: release of omega-3 fatty acids from fish oil. J Am Oil Chem Soc 2012, 89:97-102.
26. Cabrera Z., Gutarra M.L.E., Guisan J.M., Palomo J.M. Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine. Catal Commun 2010, 11:964-967.
27. Cabrera Z., Fernandez-Lorente G., Fernandez-Lafuente R., Palomo J.M., Guisan J.M. Enhancement of Novozym-435 catalytic properties by physical or chemical modification. Process Biochem 2009, 44:226-231.
28. Fuentes M., Pessela B.C.C., Maquiese J.V., Ortiz C., Segura R.L., Palomo J.M., et al. Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate-dextran. Biotechnol Prog 2004, 20:1134-1139.
29. Mateo C., Abian O., Fernandez-Lafuente R., Guisan J.M. Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites. Biotechnol Bioeng 2000, 68:98-105.
30. Garcia-Galan C., Barbosa O., Fernandez-Lafuente R. Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine. Enzyme Microb Technol 2013, 52:211-217.
31. Bolivar J.M., Rocha-Martin J., Mateo C., Cava F., Berenguer J., Fernandez-Lafuente R., et al. Coating of soluble and immobilized enzymes with ionic polymers: full stabilization of the quaternary structure of multimeric enzymes. Biomacromolecules 2009, 10:742-747.
32. Fernandez-Lafuente R. Stabilization of multimeric enzymes: strategies to prevent subunit dissociation. Enzyme Microb Technol 2009, 45:405-418.
33. Andersson M.M., Hatti-Kaul R. Protein stabilising effect of polyethyleneimine. J Biotechnol 1999, 72:21-31.
34. Bryjak J. Storage stabilization of enzyme activity by poly(ethyleneimine). Bioprocess Engineering 1995, 13:177-181.
35. Mateo C., Fernandes B., Van Rantwijk F., Stolz A., Sheldon R.A. Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine). J Mol Catal B: Enzym 2006, 38:154-157.
36. Wilson L., Illanes A., Abián O., Pessela B.C.C., Fernández-Lafuente R., Guisán J.M. Co-aggregation of penicillin G acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media. Biomacromolecules 2004, 5:852-857.
37. Fernandez-Lafuente R., Rosell C.M., Caanan-Haden L., Rodes L., Guisan J.M. Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: dramatic stabilization of penicillin acylase versus organic solvents. Enzyme Microb Technol 1999, 24:96-103.
38. Fernandez-Lafuente R., Armisén P., Sabuquillo P., Fernández-Lorente G., Guisán J.M. Immobilization of lipases by selective adsorption on hydrophobic supports. Chem Phys Lipids 1998, 93:185-197.
39. Fernández-Lorente G., Palomo J.M., Cabrera Z., Guisán J.M., Fernández-Lafuente R. Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports. Enzyme Microb Technol 2007, 41:565-569.
40. Schnapp J., Shalitin Y. Immobilization of enzymes by covalent binding to amine supports via cyanogen bromide activation. Biochem Biophys Res Commun 1976, 70:8-14.
41. Mateo C., Abian O., Bernedo M., Cuenca E., Fuentes M., Fernandez-Lorente G., et al. Some special features of glyoxyl supports to immobilize proteins. Enzyme Microb Technol 2005, 37:456-462.
42. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976, 72:248-254.
43. Carrasco-López C., Godoy C., de las Rivas B., Fernández-Lorente G., Palomo J.M., Guisán J.M., et al. Activation of bacterial thermo alkalophilic lipases is spurred by dramatic structural rearrangements. J Biol Chem 2009, 284:4365-4372.
44. Fernández-Lorente G., Palomo J.M., Mateo C., Munilla R., Ortiz C., Cabrera Z., et al. Glutaraldehyde cross-linking of lipases adsorbed on aminated supports in the presence of detergents leads to improved performance. Biomacromolecules 2006, 7:2610-2615.