Immobilization of lecitase® ultra onto a novel polystyrene DA-201 resin: Characterization and biochemical properties

Applied Biochemistry and Biotechnology

Volumn 168, Issue 5, 2012, Pages 1108-1120

  Liu, Ning ^a   Fu, Min ^a   Wang, Yong ^b   Zhao, Qiangzhong ^a   Sun, Weizheng ^a   Zhao, Mouming ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b JINAN UNIVERSITY   (China)

Author keywords

Adsorption; Esterification; Ftir; Immobilization; Lecitase ultra

Indexed keywords

ADSORPTION PROCESS; BIOCHEMICAL PROPERTIES; ENZYME CONCENTRATIONS; ESTERIFICATION REACTIONS; FREEFORMS; FTIR; FTIR SPECTROSCOPY; HELIX CONTENT; HYDROPHOBIC SUPPORTS; IMMOBILIZATION CONDITIONS; IMMOBILIZATION EFFECTS; IMMOBILIZED ENZYME; INTERFACIAL ADSORPTION; KINETIC BEHAVIOR; KINETIC CONSTANT; LANGMUIR AND FREUNDLICH EQUATIONS; LECITASE; LIPASE ACTIVITY; MACROPOROUS RESIN; PHOSPHOLIPASES; POLYSTYRENE SUPPORT; SCANNING ELECTRON MICROGRAPHS; SECONDARY CONFORMATION;

ADSORPTION; CONFORMATIONS; ESTERIFICATION; ESTERS; FOURIER TRANSFORM INFRARED SPECTROSCOPY; GLYCEROL; OLEIC ACID; POLYSTYRENES; RADIOACTIVE WASTE VITRIFICATION; RESINS; REUSABILITY; SCANNING ELECTRON MICROSCOPY; THERMOGRAVIMETRIC ANALYSIS;

ENZYME IMMOBILIZATION;

GLYCEROL; LECITASE; OLEIC ACID; PHOSPHOLIPASE; POLYSTYRENE; RESIN; TRANSITION ELEMENT; UNCLASSIFIED DRUG;

ADSORPTION; ALPHA HELIX; ARTICLE; BETA SHEET; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; ENZYME SUBSTRATE; ESTERIFICATION; HYDROPHOBICITY; INFRARED SPECTROSCOPY; ISOTHERM; PH; SCANNING ELECTRON MICROSCOPY; THERMOGRAVIMETRY; THERMOSTABILITY;

ANION EXCHANGE RESINS; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; KINETICS; LIPIDS; PHOSPHOLIPASES; POLYSTYRENES; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TEMPERATURE;

EID: 84871905734     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-012-9845-y     Document Type: Article

References (28)

1. Bojsen, K., Svendsen, A., Fuglsang, C. C., Patear, S., Borch, K., Vind, J., et al.. (2000). Novozymes A/S, Denmark. PCT International Application WO2000/32758.
2. Fernandez-Lorente, G., Filice, M., Terreni, M., Guisan, J. M., Fernandez-Lafuente, R., & Palomo, J. M. (2008). Lecitase® ultra as regioselective biocatalyst in the hydrolysis of fully protected carbohydrates strong modulation by using different immobilization protocols. Journal of Molecular Catalysis B: Enzymatic, 51, 110-117.
3. Sheelu, G., Kavitha, G., & Fadnavis, N. W. (2008). Efficient immobilization of lecitase in gelatin hydrogel and degumming of rice bran oil using a spinning basket reactor. Journal of the American Oil Chemists' Society, 85, 739-748.
4. Yang, B., Wang, Y. H., & Yang, J. G. (2006). Optimization of enzymatic degumming process for rapeseed oil. Journal of the American Oil Chemists' Society, 83, 653-658.
5. Mishra, M. K., Kumaraguru, T., Sheelu, G., & Fadnavis, N.W. (2009). Lipase activity of Lecitase® Ultra: characterization and applications in enantioselective reactions. Tetrahedron: Asymmetry, 20, 2854-2860.
6. Wang, Y., Zhao, M., Song, K., Wang, L., Tang, S., &William, W. R. (2010). Partial hydrolysis of soybean oil by phospholipase A1 (Lecitase Ultra). Food Chemistry, 121, 1066-1072.
7. Liu, N., Wang, Y., Zhao, Q., Zhang, Q., & Zhao, M. (2011). Fast synthesis of 1, 3-DAG by Lecitase® Ultra-catalyzed esterification in solvent-free system. European Journal of Lipid Science and Technology, 113, 973-979.
8. Iyer, P. V., & Ananthanarayan, L. (2008). Enzyme stability and stabilization-aqueous and non-aqueous environment. Process Biochemistry, 43, 1019-1032.
9. Fernandez-Lorente, G., Palomo, J. M., Guisan, J. M., & Fernandez-Lafuente, R. (2007). Effect of the immobilization protocol in the activity, stability, and enantioselectivity of Lecitase® Ultra. Journal of Molecular Catalysis B: Enzymatic, 47, 99-104.
10. Cabrera, Z., Fernandez-Lorente, G., Palomo, J. M., Guisan, J. M., & Fernandez-Lafuente, R. (2008). Asymmetric hydrolysis of dimethyl 3-phenylglutarate catalyzed by Lecitase Ultra®: effect of the immobilization protocol on its catalytic properties. Enzyme and Microbial Technology, 43, 531-536.
11. Garcia-Galan, C., Berenguer-Murcia, A., Fernandez-Lafuente, R., & Rodrigues, R. C. (2011). Potential of different enzyme immobilization strategies to improve enzyme performance. Advanced Synthesis and Catalysis, 353, 2885-2904.
12. Mateo, C., Palomo, J. M., Fernandez-Lorente, G., Guisan, J. M., & Fernandez-Lafuente, R. (2007). Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme and Microbial Technology, 40, 1451-1463.
13. Hernandez, K., Garcia-Galan, C. & Fernandez-Lafuente, R. (2011). Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads. Enzyme and Microbial Technology, 49, 72-78.
14. Liu, T., Liu, Y., Wang, X., Li, Q., Wang, J., & Yan, Y. (2011). Improving catalytic performance of Burkholderia cepacia lipase immobilized on macroporous resin NKA. Journal of Molecular Catalysis B: Enzymatic, 71, 45-50.
15. Shi, Z., & Shi, R. (2005). The application of resin for adsorption and separation in medical industry. Beijing: Chemical Industry.
16. Cheirsilp, B., Jeamjounkhaw, P., & H-Kittikun, A. (2009). Optimizing an alginate immobilized lipase for monoacylglycerol production by the glycerolysis reaction. Journal of Molecular Catalysis B: Enzymatic, 59, 206-211.
17. Byler, D. M., & Susi, H. (1986). Examination of the secondary structure of proteins by deconvolved FTIR spectra. Biopolymers, 25, 469-487.
18. Gao, Y., Tan, T. W., Nie, K. L., & Wang, F. (2006). Immobilization of lipase on macroporous resin and its application in synthesis of biodiesel in low aqueous media. Chinese Journal of Biotechnology, 22, 114- 118.
19. Rodrigues, R. C., & Fernandez-Lafuente, R. (2010). Lipase from Rhizomucor miehei as a biocatalyst in fats and oils modification. Journal of Molecular Catalysis B: Enzymatic, 66, 15-32.
20. Fernandez-Lorente, G., Palomo, J. M., Cabrera, Z., Guisan, J. M., & Fernandez-Lafuente, R. (2007). Specificity enhancement towards hydrophobic substrates by immobilization of lipases by interfacial activation on hydrophobic supports. Enzyme and Microbial Technology, 41, 565-569.
21. Wilson, L., Palomo, J. M., Fernandez-Lorente, G., Illanes, A., Guisan, J. M., & Fernandez-Lafuente, R. (2006). Effect of lipase-lipase interactions in the activity, stability and specificity of a lipase from Alcaligenes sp. Enzyme and Microbial Technology, 39, 259-264.
22. Al-Duri, B., & Yong, Y. P. (1997). Characterisation of the equilibrium behaviour of lipase PS (from Pseudomonas) and lipolase 100L (from Humicola) onto Accurel EP100. Journal of Molecular Catalysis B: Enzymatic, 3, 177-188.
23. Hoshino, K., Taniguchi, M., Katagiri, M., & Fujii, M. (1992). Properties of amylase immobilized on a new reversibly soluble-insoluble polymer and its application to repeated hydrolysis of soluble starch. Journal of Chemical Engineering of Japan, 25, 569-574.
24. Serefoglou, E., Litina, K., Gournis, D., Kalogeris, E., Tzialla, A. A., Pavlidis, I. V., et al. (2000). Smectite clays as solid supports for immobilization of glucosidase: synthesis, characterization, and biochemical properties. Chemistry of Materials, 28, 4106-4115.
25. Kharrat, N., Ali, Y. B., Marzouk, S., Gargouri, Y., & Karra-Chaabouni, M. (2011). Immobilization of Rhizopus oryzae lipase on silica aerogels by adsorption: comparison with the free enzyme. Process Biochemistry, 46, 1083-1089.
26. Peng, R., Lin, J., & Wei, D. (2010). Purification and characterization of an organic solventtolerant lipase from Pseudomonas aeruginosa CS-2. Applied Biochemistry and Biotechnology, 162, 733-743.
27. Rahman, R. N. Z. R. A., Bahrum, S. N., Salleh, A. B., & Basri, M. (2006). S5 lipase: an organic solvent tolerant enzyme. Journal of Microbiology, 44, 583-590.
28. Brady, D., & Jordaan, J. (2009). Advances in enzyme immobilisation. Biotechnology Letters, 31, 1639- 1650.