Enzymatic treatment of specimens before DNA extraction directly influences molecular detection of infectious agents

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Goldschmidt, Pablo ^a   Degorge, Sandrine ^a   Merabet, Lilia ^a   Chaumeil, Christine ^a  

^a CENTRE HOSPITALIER NATIONAL D OPHTALMOLOGIE DES QUINZE VINGTS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; ENZYME; FUNGAL DNA; LYTICASE; PROTEINASE K; UNCLASSIFIED DRUG; VIRUS DNA; GLUCAN ENDO 1,3 BETA GLUCOSIDASE; MULTIENZYME COMPLEX; PEPTIDE HYDROLASE;

ANALYTICAL EQUIPMENT; ARTICLE; ASPERGILLUS NIGER; BACTERIUM DETECTION; BINDING AFFINITY; CANDIDA ALBICANS; CHEMICAL REACTION; COLUMN BEAD; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; ELUTION; ENZYMATIC PRETREATMENT; ESCHERICHIA COLI; FUNGAL DETECTION; FUSARIUM SOLANI; GENE AMPLIFICATION; HERPES VIRUS; HIGH RESOLUTION MELTING ANALYSIS; LABORATORY DEVICE; MAGNETIC BEAD; MICROORGANISM DETECTION; MOLECULAR DIAGNOSIS; NONHUMAN; PROPIONIBACTERIUM ACNES; PROTEIN DEGRADATION; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; STAPHYLOCOCCUS EPIDERMIDIS; STREPTOCOCCUS MITIS; VIRUS DETECTION; ASCOMYCETES; BACTERIUM; GENETICS; HUMAN; ISOLATION AND PURIFICATION; SENSITIVITY AND SPECIFICITY;

ASCOMYCOTA; BACTERIA; DNA, BACTERIAL; DNA, FUNGAL; ENDOPEPTIDASE K; GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE; HUMANS; MULTIENZYME COMPLEXES; PEPTIDE HYDROLASES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84903287067     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094886     Document Type: Article

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