Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate

Journal of Clinical Microbiology

Volumn 36, Issue 10, 1998, Pages 2810-2816

  Fredricks, David N ^a,b   Relman, David A ^a,b  

^a STANFORD UNIVERSITY MEDICAL CENTER   (United States)

^b VA PALO ALTO HEALTH CARE SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; POLYANETHOLESULFONIC ACID;

ARTICLE; BACTERIAL GENETICS; BACTERIUM CULTURE; BLOOD CULTURE; ESCHERICHIA COLI; GENE AMPLIFICATION; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0031660552     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.36.10.2810-2816.1998     Document Type: Article

References (26)

1. Ahokas, H., and M. J. Erkkila. 1993. Interference of PCR amplification by the polyamines, spermine and spermidine. PCR Methods Appl. 3:65-68.
2. Akane, A., K. Matsubara, H. Nakamura, S. Takahashi, and K. Kimura. 1994. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J. Forensic Sci. 39:362-372.
3. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
4. Benson, D. A., M. S. Boguski, D. J. Lipman, J. Ostell, and B. F. Ouellette. 1998. GenBank. Nucleic Acids Res. 26:1-7.
5. Brosius, J., T. J. Dull, D. D. Sleeter, and H. F. Noller. 1981. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148:107-127.
6. Demeke, T., and R. P. Adams. 1992. The effects of plant polysaccharides and buffer additives on PCR. BioTechniques 12:332-334.
7. Edberg, S. C., and M. K. Edberg. 1983. Inactivation of the polyanionic detergent sodium polyanetholsulfonate by hemoglobin. J. Clin. Microbiol. 18:1047-1050.
8. Escamilla, J., L. T. Santiago, C. V. Uylangco, and J. H. Cross. 1983. Evaluation of sodium polyanethanol sulfonate as a blood culture additive for recovery of Salmonella typhi and Salmonella paratyphi A. J. Clin. Microbiol. 18:380-383.
9. Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17:368-376.
10. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791.
11. Forbes, B. A., and K. E. Hicks. 1994. Ability of PCR assay to identify Mycobacterium tuberculosis in BACTEC 12B vials. J. Clin. Microbiol. 32: 1725-1728.
12. Greenfield, L., and T. J. White. 1993. Sample preparation methods, p. 122-137. In D. H. Persing, T. F. Smith, F. C. Tenover, and T. J. White (ed.) Diagnostic molecular microbiology. American Society for Microbiology, Washington, D.C.
13. Hassan, K. M., I. Baldeh, O. Secka, A. Falade, and B. Greenwood. 1994. Detection of Streptococcus pneumoniae DNA in blood cultures by PCR. J. Clin. Microbiol. 32:1721-1724.
14. Heinen, W., A. M. Lauwers, and J. W. Mulders. 1982. Bacillus flavothermus, a newly isolated facultative thermophile. Antonie Leeuwenhoek 48:265-272.
15. Holodniy, M., S. Kim, D. Katzenstein, M. Konrad, E. Groves, and T. C. Merigan. 1991. Inhibition of human immunodeficiency virus gene amplification by heparin. J. Clin. Microbiol. 29:676-679.
16. Khan, G., H. O. Kangro, P. J. Coates, and R. B. Heath. 1991. Inhibitory effects of urine on the polymerase chain reaction for cytomegalovirus DNA. J. Clin. Pathol. 44:360-365.
17. Krogstad, D. J., P. R. Murray, G. G. Granich, A. C. Niles, J. H. Ladenson, and J. E. Davis. 1981. Sodium polyanethol sulfonate inactivation of amino-glycosides. Antimicrob. Agents Chemother. 20:272-274.
18. Kulski, J. K., C. Khinsoe, T. Pryce, and K. Christiansen. 1995. Use of a multiplex PCR to detect and identify Mycobacterium avium and M. intracellulare in blood culture fluids of AIDS patients. J. Clin. Microbiol. 33:668-674.
19. Kulski, J. K., and T. Pryce. 1996. Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection. J. Clin. Microbiol. 34:1985-1991.
20. Maidak, B. L., G. J. Olsen, N. Larsen, R. Overbeek, M. J. McCaughey, and C. R. Woese. 1997. The RDP (Ribosomal Database Project). Nucleic Acids Res. 25:109-111.
21. Olsen, G. J., H. Matsuda, R. Hagstrom, and R. Overbeek. 1994. fastD-NAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput. Appl. Biosci. 10:41-48.
22. Tenney, J. H., L. B. Reller, W. L. Wang, R. L. Cox, and S. Mirrett. 1982. Comparative evaluation of supplemented peptone broth with sodium polyanetholesulfonate and Trypticase soy broth with sodium amylosulfate for detection of septicemia. J. Clin. Microbiol. 16:107-110.
23. Traub, W. H., and P. I. Fukushima. 1978. Neutralization of human serum lysozyme by sodium polyanethol sulfonate but not by sodium amylosulfate. J. Clin. Microbiol. 8:306-312.
24. Traub, W. H., and P. I. Fukushima. 1979. Variable neutralization of several nonspecific antibacterial systems in fresh, defibrinated human blood by sodium polyanetholsulfonate and sodium amylosulfate. J. Clin. Microbiol. 10: 27-31.
25. Traub, W. H., and I. Kleber. 1977. Inactivation of classical and alternative pathway-activated bactericidal activity of human serum by sodium polyanetholsulfonate. J. Clin. Microbiol. 5:278-284.
26. Wiedbrauk, D. L., J. C. Werner, and A. M. Drevon. 1995. Inhibition of PCR by aqueous and vitreous fluids. J. Clin. Microbiol. 33:2643-2646.