The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples

Journal of Infection and Chemotherapy

Volumn 17, Issue 3, 2011, Pages 327-333

  Obara, Hideaki ^a   Aikawa, Naoki ^a   Hasegawa, Naoki ^a   Hori, Shingo ^a   Ikeda, Yasuo ^a   Kobayashi, Yoshio ^a   Murata, Mitsuru ^a   Okamoto, Shinichiro ^a   Takeda, Junzo ^a   Tanabe, Minoru ^a   Sakakura, Yasuhiko ^c   Ginba, Hiroyuki ^c   Kitajima, Masaki ^a,b   Kitagawa, Yuko ^a  

^a KEIO UNIVERSITY   (Japan)

^b INTERNATIONAL UNIVERSITY OF HEALTH AND WELFARE   (Japan)

^c Roche Diagnostics KK   (Japan)

Author keywords

Blood culture; Real time polymerase chain reaction (RT PCR); Sepsis; Systemic inflammatory response syndrome (SIRS)

Indexed keywords

METICILLIN;

ACINETOBACTER BAUMANNII; ACINETOBACTER CALCOACETICUS; ADULT; AGED; ARTICLE; BACTERIAL INFECTION; BACTERIUM IDENTIFICATION; BLOOD CULTURE; BLOOD SAMPLING; CANDIDA ALBICANS; COLONY FORMING UNIT; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; EARLY DIAGNOSIS; ENTEROBACTER CLOACAE; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; FEMALE; FUNGUS IDENTIFICATION; HUMAN; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MALE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 80051586700     PISSN: 1341321X     EISSN: 14377780     Source Type: Journal    
DOI: 10.1007/s10156-010-0168-z     Document Type: Article

References (22)

1. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med. 2001;29:1303-10. (Pubitemid 32641789)
2. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ. Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med. 2008;36:1487-92. (Pubitemid 351620647)
3. Kollef MH. Inadequate antimicrobial treatment: an important determinant of outcome for hospitalized patients. Clin Infect Dis. 2000;31(Suppl 4):S131-8. (Pubitemid 32291120)
4. Harbarth S, Garbino J, Pugin J, Romand JA, Lew D, Pittet D. Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am J Med. 2003;115:529-35. (Pubitemid 37352798)
5. Wellinghausen N, Wirths B, Franz AR, Karolyi L, Marre R, Reischl U. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn Microbiol Infect Dis. 2004;48:229-41. (Pubitemid 38519579)
6. Bone RC. Toward an epidemiology and natural history of SIRS (systemic inflammatory response syndrome). JAMA. 1992;268:3452-5. (Pubitemid 23002750)
7. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA. 1995;273:117-23.
8. Peterson LR, Dalhoff A. Towards targeted prescribing: will the cure for antimicrobial resistance be specific, directed therapy through improved diagnostic testing? J Antimicrob Chemother. 2004;53:902-5. (Pubitemid 38807629)
9. Avolio M, Diamante P, Zamparo S, Modolo ML, Grosso S, Zigante P, et al. Molecular identification of bloodstream pathogens in patients presenting to the emergency department with suspected sepsis. Shock. 2010;34:27-30.
10. Wallet F, Nseir S, Baumann L, Herwegh S, Sendid B, Boulo M, et al. Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect. 2010;16:774-9.
11. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, Salzberger B. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis. 2009;9:126.
12. Cockerill FR 3rd, Hughes JG, Vetter EA, Mueller RA, Weaver AL, Ilstrup DM, et al. Analysis of 281, 797 consecutive blood cultures performed over an eight-year period: trends in microorganisms isolated and the value of anaerobic culture of blood. Clin Infect Dis. 1997;24:403-18. (Pubitemid 27096795)
13. Paterson DL, Rice LB. Empirical antibiotic choice for the seriously ill patient: are minimization of selection of resistant organisms and maximization of individual outcome mutually exclusive? Clin Infect Dis. 2003;36:1006-12. (Pubitemid 36513286)
14. Headley AS, Tolley E, Meduri GU. Infections and the inflammatory response in acute respiratory distress syndrome. Chest. 1997;111:1306-21. (Pubitemid 27200090)
15. Klaschik S, Lehmann LE, Raadts A, Book M, Hoeft A, Stuber F. Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria. J Clin Microbiol. 2002;40:4304-7. (Pubitemid 35285831)
16. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med. 2006;34:1589-96. (Pubitemid 43810035)
17. Jordan JA, Durso MB. Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn. 2005;7:575-81. (Pubitemid 41601508)
18. Cursons RT, Jeyerajah E, Sleigh JW. The use of polymerase chain reaction to detect septicemia in critically ill patients. Crit Care Med. 1999;27:937-40. (Pubitemid 29266011)
19. Wheeler J, Murphy OM, Freeman R, Kearns AM, Steward M, Lee MJ. PCR can add to detection of pneumococcal disease in pneumonic patients receiving antibiotics at admission. J Clin Microbiol. 2000;38:3907.
20. Vince A, Lepej SZ, Barsic B, Dusek D, Mitrovic Z, Serventi-Seiwerth R, et al. LightCycler SeptiFast assay as a tool for the rapid diagnosis of sepsis in patients during antimicrobial therapy. J Med Microbiol. 2008;57:1306-7.
21. Mermel LA, Maki DG. Detection of bacteremia in adults: consequences of culturing an inadequate volume of blood. Ann Intern Med. 1993;119:270-2. (Pubitemid 23319882)
22. Shafazand S, Weinacker AB. Blood cultures in the critical care unit: improving utilization and yield. Chest. 2002;122:1727-36. (Pubitemid 35366089)