Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization

Journal of Clinical Microbiology

Volumn 43, Issue 7, 2005, Pages 3390-3397

  Poppert, Sven ^a   Essig, Andreas ^a   Stoehr, Barbara ^a   Steingruber, Adelinde ^a   Wirths, Beate ^a   Juretschko, Stefan ^c,d   Reischl, Udo ^b   Wellinghausen, Nele ^a  

^a UNIVERSITY OF ULM   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

^c UNIVERSITY OF WASHINGTON   (United States)

^d ARKANSAS CHILDREN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHM; AMPLICON; ARTICLE; BACTERIAL MENINGITIS; CEREBROSPINAL FLUID EXAMINATION; EARLY DIAGNOSIS; EUBACTERIUM; FLUORESCENCE IN SITU HYBRIDIZATION; HAEMOPHILUS INFLUENZAE; LABORATORY DIAGNOSIS; MICROSCOPY; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; STREPTOCOCCUS AGALACTIAE;

CEREBROSPINAL FLUID; CULTURE MEDIA; DNA PROBES; DNA, BACTERIAL; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE COCCI; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MENINGITIS, BACTERIAL; MICROSCOPY; POLYMERASE CHAIN REACTION; TIME FACTORS;

HAEMOPHILUS INFLUENZAE; NEISSERIA MENINGITIDIS; STREPTOCOCCUS AGALACTIAE;

EID: 22144485955     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.7.3390-3397.2005     Document Type: Article

References (30)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
3. Balganesh, M., M. K. Lalitha, and R. Nathaniel. 2000. Rapid diagnosis of acute pyogenic meningitis by a combined PCR dot-blot assay. Mol. Cell. Probes 14:61-69.
4. Fuchs, B. M., G. Wallner, W. Beisker, I. Schwippl, W. Ludwig, and R. Amann. 1998. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl. Environ. Microbiol. 64:4973-4982.
5. Greisen, K., M. Loeffelholz, A. Purohit, and D. Leong. 1994. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Clin. Microbiol. 32:335-351.
6. Hall, L. M., B. Duke, and G. Urwin. 1995. An approach to the identification of the pathogens of bacterial meningitis by the polymerase chain reaction. Eur. J. Clin. Microbiol. Infect. Dis. 14:1090-1094.
7. Hogardt, M., K. Trebesius, A. M. Geiger, M. Hornef, J. Rosenecker, and J. Heesemann. 2000. Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J. Clin. Microbiol. 38:818-825.
8. Hussein, A. S., and S. D. Shafran. 2000. Acute bacterial meningitis in adults. A 12-year review. Medicine (Baltimore) 79:360-368.
9. Jansen, G. J., M. Mooibroek, J. Idema, H. J. Harmsen, G. W. Welling, and J. E. Degener. 2000. Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes. J. Clin. Microbiol. 38: 814-817.
10. Ke, D., C. Menard, F. J. Picard, M. Boissinot, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2000. Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin. Chem. 46:324-331.
11. Kearns, A. M., R. Freeman, O. M. Murphy, P. R. Seiders, M. Steward, and J. Wheeler. 1999. Rapid PCR-based detection of Streptococcus pneumoniae DNA in cerebrospinal fluid. J. Clin. Microbiol. 37:3434.
12. Kempf, V. A., K. Trebesius, and I. B. Autenrieth. 2000. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38:830-838.
13. Kotilainen, P., J. Jalava, O. Meurman, O. P. Lehtonen, E. Rintala, O. P. Seppala, E. Eerola, and S. Nikkari. 1998. Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid. J. Clin. Microbiol. 36:2205-2209.
14. Lu, J. J., C. L. Perng, S. Y. Lee, and C. C. Wan. 2000. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid. J. Clin. Microbiol. 38:2076-2080.
15. Manz W., R. Amann, W. Ludwig, M. Wagner, and K.-H. Schleifer. 1992 Phylogenetic oligodeoxynucleotide probes for the major subclasses of Proteobacteria: problems and solutions. Syst. Appl. Microbiol. 1:593-600.
16. Newcombe, J., K. Cartwright, W. H. Palmer, and J. McFadden. 1996. PCR of peripheral blood for diagnosis of meningococcal disease. J. Clin. Microbiol. 34:1637-1640.
17. Oliveira, K., G. W. Procop, D. Wilson, J. Coull, and H. Stender. 2002. Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J. Clin. Microbiol. 40:247-251.
18. Perry-O'Keefe, H., H. Stender, A. Broomer, K. Oliveira, J. Coull, and J. J. Hyldig-Nielsen. 2001. Filter-based PNA in situ hybridization for rapid detection, identification and enumeration of specific micro-organisms. J. Appl. Microbiol. 90:180-189.
19. Poppert, S., A. Essig, R. Marre, M. Wagner, and M. Horn. 2002. Detection and differentiation of chlamydiae by fluorescence in situ hybridization. Appl. Environ. Microbiol. 68:4081-4089.
20. Reischl, U., H. J. Linde, B. Leppmeier, N. Lehn. 2002. Duplex LightCycler PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation, p. 93-105. In U. Reischl, C. Wittwer, and F. Cockerill (ed.), Rapid cycle real-time PCR. Springer Verlag, Berlin, Germany.
21. Richardson, D. C., L. Louie, M. Louie, and A. E. Simor. 2003. Evaluation of a rapid PCR assay for diagnosis of meningococcal meningitis. J. Clin. Microbiol. 41:3851-3853.
22. Saez-Llorens, X., and G. H. McCracken, Jr. 2003. Bacterial meningitis in children. Lancet 361:2139-2148.
23. Saravolatz, L. D., O. Manzor, N. VanderVelde, J. Pawlak, and B. Belian. 2003. Broad-range bacterial polymerase chain reaction for early detection of bacterial meningitis. Clin. Infect. Dis. 36:40-45.
24. Schuchat, A., K. Robinson, J. D. Wenger, L. H. Harrison, M. Farley, A. L. Reingold, L. Lefkowitz, and B. A. Perkins. 1997. Bacterial meningitis in the United States in 1995. Active Surveillance Team. N. Engl. J. Med. 337:970-976.
25. Segreti, J., and A. A. Harris. 1996. Acute bacterial meningitis. Infect. Dis. Clin. N. Am. 10:797-809.
26. Stevens, J. P., M. Eames, A. Kent, S. Halket, D. Holt, and D. Harvey. 2003. Long term outcome of neonatal meningitis. Arch. Dis. Child. Fetal Neonatal Ed. 88:F179-F184.
27. Trebesius, K., L. Leitritz, K. Adler, S. Schubert, I. B. Autenrieth, and J. Heesemann. 2000. Culture independent and rapid identification of bacterial pathogens in necrotising fasciitis and streptococcal toxic shock syndrome by fluorescence in situ hybridisation. Med. Microbiol. Immunol. (Berlin) 188:169-175.
28. Trotha, R., T. Hanck, W. Konig, and B. Konig. 2001. Rapid ribosequencing - an effective diagnostic tool for detecting microbial infection. Infection 29:12-16.
29. van Haeften, R., S. Palladino, I. Kay, T. Keil, C. Heath, and G. W. Waterer. 2003. A quantitative LightCycler PCR to detect Streptococcus pneumoniae in blood and CSF. Diagn. Microbiol. Infect. Dis. 47:407-414.
30. Wellinghausen, N., B. Wirths, A. R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time LightCycler polymerase chain reaction (PCR) with sequence-specific probes. Diagn. Microbiol. Infect. Dis. 48:229-241.