Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis

Mucosal Immunology

Volumn 7, Issue 4, 2014, Pages 818-828

  Moon, C ^a   Vandussen, K L ^a   Miyoshi, H ^a   Stappenbeck, T S ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; IMMUNOGLOBULIN A; INTERLEUKIN 17; INTERLEUKIN 1BETA; MUCIN 2; PROTEIN ZO1; SECRETORY COMPONENT; SYNDECAN 1; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; VILLIN; VILLIN 1; CYTOKINE; LIPOPOLYSACCHARIDE; TOLL LIKE RECEPTOR 4;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL DEATH; CELL DENSITY; CELL DIFFERENTIATION; CONTROLLED STUDY; ELECTRIC RESISTANCE; ENZYME INHIBITION; EPITHELIAL CELL MONOLAYER CULTURE SYSTEM; ESCHERICHIA COLI; FILTERS AND MEMBRANES; IMMUNOMODULATION; INTESTINE CELL; INTESTINE EPITHELIUM CELL; LIMIT OF DETECTION; MONOLAYER CULTURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; TRANSCYTOSIS; ANIMAL; COLON; DRUG EFFECTS; EPITHELIUM CELL; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; INTESTINE MUCOSA; KNOCKOUT MOUSE; METABOLISM; PHYSIOLOGY; PRIMARY CELL CULTURE; PROCEDURES;

ANIMALS; COLON; CYTOKINES; EPITHELIAL CELLS; GENE EXPRESSION; GENE EXPRESSION REGULATION; IMMUNOGLOBULIN A; INTESTINAL MUCOSA; LIPOPOLYSACCHARIDES; MICE; MICE, KNOCKOUT; PRIMARY CELL CULTURE; RECEPTORS, POLYMERIC IMMUNOGLOBULIN; TOLL-LIKE RECEPTOR 4; TRANSCYTOSIS;

EID: 84902603134     PISSN: 19330219     EISSN: 19353456     Source Type: Journal    
DOI: 10.1038/mi.2013.98     Document Type: Article

References (47)

1. Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262-265 (2009
2. Miyoshi, H., Ajima, R., Luo, C.T., Yamaguchi, T.P. & Stappenbeck, T.S. Wnt5a potentiates TGF-beta signaling to promote colonic crypt regeneration after tissue injury. Science 338, 108-113 (2012
3. Sambuy, Y. et al. The Caco-2 cell line as a model of the intestinal barrier: Influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol. Toxicol. 21, 1-26 (2005
4. Meunier, V., Bourrie, M., Berger, Y. & Fabre, G. The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications. Cell Biol. Toxicol. 11, 187-194 (1995
5. Rousset, M. The human colon carcinoma cell lines HT-29 and Caco-2: Two in vitro models for the study of intestinal differentiation. Biochimie 68, 1035-1040 (1986
6. Duizer, E., Penninks, A.H., Stenhuis, W.H. & Groten, J.P. Comparison of permeability characteristics of the human colonic Caco-2 and rat small intestinal IEC-18 cell lines. J. Control Rel. 49, 39-49 (1997
7. Irvine, J.D. et al. MDCK (Madin-Darby canine kidney) cells: A tool for membrane permeability screening. J. Pharm. Sci. 88, 28-33 (1999
8. Kuratnik, A. & Giardina, C. Intestinal organoids as tissue surrogates for toxicological and pharmacological studies. Biochem. Pharmacol. 85, 1721-1726 (2013
9. Sun, H., Chow, E.C.Y., Liu, S., Du, Y. & Pang, K.S. The Caco-2 cell monolayer: Usefulness and limitations. Expert Opin. Drug Metab. 4, 395-411 (2008
10. Kaetzel, C.S. The polymeric immunoglobulin receptor: Bridging innate and adaptive immune responses at mucosal surfaces. Immunol. Rev. 206, 83-99 (2005
11. Johansen, F.E. &Kaetzel, C.S. Regulation of the polymeric immunoglobulin receptor and IgA transport: New advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity. Mucosal Immunol. 4, 598-602 (2011
12. Rojas, R. & Apodaca, G. Immunoglobulin transport across polarized epithelial cells. Nat. Rev. Mol. Cell. Biol. 3, 944-955 (2002
13. Hooper, L.V. OPINION Do symbiotic bacteria subvert host immunity? Nat. Rev. Microbiol. 7, 367-374 (2009
14. Hamburger, A.E., Bjorkman, P.J. & Herr, A.B. Structural insights into antibody-mediated mucosal immunity. Curr. Top.Microbiol. Immunol. 308, 173-204 (2006
15. Brandtzaeg, P. & Prydz, H. Direct evidence for an integrated function of J chain and secretory component in epithelial transport of immunoglobulins. Nature 311, 71-73 (1984
16. Mostov, K.E., Kraehenbuhl, J.P. & Blobel, G. Receptor-mediated transcellular transport of immunoglobulin: Synthesis of secretory component as multiple and larger transmembrane forms. Proc. Natl. Acad. Sci. USA 77, 7257-7261 (1980
17. Mostov, K.E., Verges, M. & Altschuler, Y. Membrane traffic in polarized epithelial cells. Curr. Opin. Cell Biol. 12, 483-490 (2000
18. Lindh, E. Increased resistance of immunoglobulin a dimers to proteolytic degradation after binding of secretory component. J. Immunol. 114, 284-286 (1975
19. Hooper, L.V. et al. Molecular analysis of commensal host-microbial relationships in the intestine. Science 291, 881-884 (2001
20. Bruno, M.E. et al. Regulation of the polymeric immunoglobulin receptor in intestinal epithelial cells by Enterobacteriaceae: Implications for mucosal homeostasis. Immunol. Invest. 39, 356-382 (2010
21. Schneeman, T.A. et al. Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: Linking innate and adaptive immune responses. J. Immunol. 175, 376-384 (2005
22. Blanch, V.J., Piskurich, J.F. & Kaetzel, C.S. Cutting edge: Coordinate regulation of IFN regulatory factor-1 and the polymeric Ig receptor by proinflammatory cytokines. J. Immunol. 162, 1232-1235 (1999
23. Piskurich, J.F. et al. Transcriptional regulation of the human polymeric immunoglobulin receptor gene by interferon-gamma. Mol. Immunol. 34, 75-91 (1997
24. Schjerven, H., Brandtzaeg, P. & Johansen, F.E. A novel NF-kappa B/Rel site in intron 1 cooperates with proximal promoter elements to mediate TNF-Alpha-induced transcription of the human polymeric Ig receptor. J. Immunol. 167, 6412-6420 (2001
25. Hayashi, M. et al. The polymeric immunoglobulin receptor (secretory component) in a human intestinal epithelial cell line is up-regulated by interleukin-1. Immunology 92, 220-225 (1997
26. Cao, A.T., Yao, S., Gong, B., Elson, C.O. & Cong, Y. Th17 cells upregulate polymeric Ig receptor and intestinal IgA and contribute to intestinal homeostasis. J. Immunol. 189, 4666-4673 (2012
27. VanDussen, K.L. et al. Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells. Development 139, 488-497 (2012
28. van Es, J.H. et al. Notch/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells. Nature 435, 959-963 (2005
29. Bretscher, A. & Weber, K. Villin: The majormicrofilament-Associated protein of the intestinal microvillus. Proc. Natl. Acad. Sci. USA 76, 2321-2325 (1979
30. Stevenson, B.R., Siliciano, J.D., Mooseker, M.S. & Goodenough, D.A. Identification of ZO-1: A highmolecular weight polypeptide associatedwith the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103, 755-766 (1986
31. O'Connor, D.T., Burton, D. & Deftos, L.J. Chromogranin A: Immunohistology reveals its universal occurrence in normal polypeptide hormone producing endocrine glands. Life Sci. 33, 1657-1663 (1983
32. Falk, P., Roth, K.A. & Gordon, J.I. Lectins are sensitive tools for defining the differentiation programs of mouse gut epithelial cell lineages. Am. J. Physiol. 266, G987-1003 (1994
33. Cash, H.L., Whitham, C.V., Behrendt, C.L. & Hooper, L.V. Symbiotic bacteria direct expression of an intestinal bactericidal lectin. Science 313, 1126-1130 (2006
34. Maunoury, R. et al. Villin expression in the visceral endoderm and in the gut anlage during early mouse embryogenesis. EMBO J 7, 3321-3329 (1988
35. Song, W., Bomsel, M., Casanova, J., Vaerman, J.P. & Mostov, K. Stimulation of transcytosis of the polymeric immunoglobulin receptor by dimeric IgA. Proc. Natl. Acad. Sci. USA 91, 163-166 (1994
36. Diebel, L.N. & Liberati, D.M. Disparate effects of bacteria and Toll-like receptor-dependant bacterial ligand stimulation on immunoglobulin A transcytosis. J. Trauma 70, 691-700 (2011
37. Frantz, A.L. et al. Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides. Mucosal Immunol. 5, 501-512 (2012
38. Nakamura, Y. et al. Upregulation of polymeric immunoglobulin receptor expression by the heat-inactivated potential probiotic Bifidobacterium bifidum OLB6378 in a Mouse Intestinal Explant Model. Scand. J. Immunol. 75, 176-183 (2012
39. Ezeonu, I., Wang, M., Kumar, R. & Dutt, K. Density-dependent differentiation in nontransformed human retinal progenitor cells in response to basic fibroblast growth factor- And transforming growth factor-Alpha. DNA Cell Biol. 22, 607-620 (2003
40. Lee, Y.S., Yuspa, S.H. & Dlugosz, A.A. Differentiation of cultured human epidermal keratinocytes at high cell densities is mediated by endogenous activation of the protein kinase C signaling pathway. J. Invest. Dermatol. 111, 762-766 (1998
41. McBeath, R., Pirone, D.M., Nelson, C.M., Bhadriraju, K. & Chen, C.S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev. Cell 6, 483-495 (2004
42. Mestecky, J. et al. Mucosal Immunology. (Academic Press, San Diego, CA, 2005
43. Johansen, F.E. et al. Absence of epithelial immunoglobulin A transport, with increased mucosal leakiness, in polymeric immunoglobulin receptor/ secretory component-deficient mice. J. Exp. Med. 190, 915-921 (1999
44. Poltorak, A. et al. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: Mutations in Tlr4 gene. Science 282, 2085-2088 (1998
45. Bloom, S.M. et al. Commensal Bacteroides species induce colitis in host-genotype-specific fashion in a mouse model of inflammatory bowel disease. Cell Host Microbe 9, 390-403 (2011
46. Schneider, C.A., Rasband, W.S. & Eliceiri, K.W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671-675 (2012
47. Kang, S.S. et al. An antibiotic-responsive mouse model of fulminant ulcerative colitis. PLoS Med. 5, e41 (2008.