Comparison of permeability characteristics of the human colonic Caco-2 and rat small intestinal IEC-18 cell lines

Journal of Controlled Release

Volumn 49, Issue 1, 1997, Pages 39-49

  Duizer, E ^a   Penninks, A H ^a   Stenhuis, W H ^a   Groten, J P ^a  

^a TNO   (Netherlands)

Author keywords

Caco 2; IEC 18; Paracellular transport; Tight junction

Indexed keywords

ACETYLSALICYLIC ACID; ALKALINE PHOSPHATASE; FLUORESCEIN; FLUORESCEIN ISOTHIOCYANATE DEXTRAN; ISOENZYME; MACROGOL 4000; MANNITOL; PROPRANOLOL; SALICYLIC ACID; SUCRASE ISOMALTASE; TESTOSTERONE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CELL MEMBRANE; CELL STRAIN CACO 2; COLON; COLON ABSORPTION; COLON CARCINOMA; CONTROLLED STUDY; ELECTRIC RESISTANCE; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HYDROPHILICITY; IN VITRO STUDY; INTESTINE ABSORPTION; INTESTINE CELL; MEMBRANE PERMEABILITY; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0030826965     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-3659(97)00058-8     Document Type: Article

References (37)

1. Hidalgo I.J., Raub T.J., Borchardt R.T. Characterization of the human colon carcinoma cell line (Caco-2) as a model for intestinal epithelial permeability. Gastroenterology. 96:1989;736-749.
2. Hilgers A.R., Conradi R.A., Burton P.S. Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa. Pharm. Res. 7:1990;902-910.
3. Artursson P., Karlsson J. Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells. Biochem. Biophys. Res. Commun. 175:1991;880-885.
4. Hovgaard L., Brontsted H. Drug delivery in Caco-2 monolayers. IV. Absorption enhancer effects of cyclodextrins. Pharm. Res. 9:1995;1328-1332.
5. Walter E., Kissel T. Heterogeneity in the human intestinal cell line Caco-2 leads to differences in transepithelial transport. Eur. J. Pharm. Sci. 3:1995;215-230.
6. Dharmsathaphorn K., Madara J.L. Established intestinal cell lines as model systems for electrolyte transport studies. Methods Enzymol. 192:1990;345-389.
7. Madara J.L., Stafford J., Dharmsathaphorn K., Carlson S. Structural analysis of a human intestinal epithelial cell line. Gastroenterology. 92:1987;1133-1145.
8. Karlsson J., Wikman A., Artursson P. The mucus layer as barrier to drug absorption in monolayers of human intestinal epithelial HT29-H goblet cells. Int. J. Pharm. 99:1993;209-218.
9. Wils P., Warnery A., Puhung-Ba V., Scherman D. Differentiated intestinal epithelial cell lines as in vitro models for predicting the intestinal absorption of drugs. Cell Biol. Toxicol. 10:1994;393-397.
10. Quaroni A., Wands J., Trelstad R.L., Isselbacher K.J. Epithelioid cell cultures from rat small intestine. J. Cell Biol. 80:1979;248-265.
11. Wilson G., Hassan I.F., Dix C.J., Williamson I., Shah R., Mackay M. Transport and permeability properties of human Caco-2 cells: an in vitro model of the intestinal epithelial cell barrier. J. Control. Release. 11:1990;25-40.
12. Hillgren K.M., Kato A., Borchardt R.T. In vitro systems for studying intestinal drug absorption. Med. Res. Rev. 15:1995;83-109.
13. Blomquist L., Bark T., Hedenborg G., Svenberg T., Norman A. Comparison between the lactulose/mannitol and Cr-EDTA/Mannitol methods for intestinal permeability. Scand. J. Gastroenterol. 28:1993;274-280.
14. Bijlsma P.B., Peeters R.A., Groot J.A., Dekker P.R., Taminiau J.A.J.M., vanderMeer R. Differential in vivo and in vitro intestinal permeability to lactulose and mannitol in animals and humans: a hypothesis. Gastroenterology. 108:1995;687-696.
15. Ma T.Y., Hollander D., Erickson R.A., Truong H., Nguyen H., Krugliak P. Mechanism of colonic permeation of inulin: is rat colon more permeable than small intestine? Gastroenterology. 108:1995;12-20.
16. Nellans H.N. (B) Mechanisms of peptide and protein absorption. Adv. Drug. Deliv. Rev. 7:1991;339-364.
17. Quaroni A., Isselbacher K.J. Cytotoxic effects and metabolism of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67:1981;1353-1362.
18. Ling K., Bhalla D., Hollander D. Mechanism of carrageenan injury of IEC18 small intestinal epithelial cell monolayers. Gastroenterology. 95:1988;1487-1495.
19. Ma T.Y., Hollander D., Freeman D., Nguyen T., Krugliak P. Oxygen free radical injury of IEC-18 small intestinal epithelial cell monolayers. Gastroenterology. 100:1991;1533-1543.
20. Deitch E.A., Haskel Y., Cruz N., Xu D., Kvietys P.R. Caco-2 and IEC-18 intestinal epithelial cells exert bactericidal activity through an oxidant-dependent pathway. Shock. 4:1995;345-350.
21. Ma T.Y., Hollander D., Bhalla D., Nguyen H., Krugliak P. IEC-18, a non-transformed small intestinal cell line for studying epithelial permeability. J. Lab. Clin. Med. 120:1992;329-341.
22. Tietz N.W. IFCC methods for the measurement of catalytic concentration of enzymes, part 5. Clin. Chim. Acta. 135:1983;339F-349F.
23. Hoffman W.E., Everds N., Pignatello M., Solter P.F. Automated and semiautomated analaysis of rat alkaline phosphatase isoenzymes. Toxicol. Pathol. 22:1994;633-638.
24. Dahlqvist A. Assay of intestinal disaccharidases. Scand. J. Clin. Lab. Invest. 44:1984;169-172.
25. Robinson P.J., Rapoport S.L. Size selectivity of blood-brain barrier permeability at various times after osmotic opening. Am. J. Physiol. 253:1987;R459-R466.
26. Groten J.P., Luten J.B., Bruggeman I.M., Temmink J.H.M., van Bladeren P.J. Comparative toxicity and accumulation of cadmium chloride and cadmium methallothionein in primary cells and cell lines of rat intestine, liver and kidney. Toxic. In Vitro. 6:1992;509-517.
27. Chantert I., Rodolosse A., Barbat A., Dussaulx E., Brot-Laroche E., Zweibaum A., Rousset M. Differential expression of sucrase isomaltase in clones isolated from early and late passages of the cell line Caco-2: evidence for glucose-dependent negative regulation. J. Cell Sci. 107:1994;213-225.
28. Pinto M., Robine-Leon S., Appay M.D., Kedinger M., Triadou N., Dussaulx E., Lacroix B., Simon-Assman P., Haffen K., Fogh J., Zweibaum A. Enterocyte-like differentiation and polarization of the human colon carcinoma cell lined Caco-2 in culture. Biol. Cell. 47:1983;323-330.
29. Claude P. Morphologic factors influencing transepithelial permeability: a model for the resistance of the zonula occludens. J. Membr. Biol. 39:1987;219-232.
30. Stevenson B.R., Anderson J.M., Goodenough D.A., Mooseker M.S. Tight junctional structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance. J. Cell Biol. 107:1988;2401-2408.
31. Powell D.W. Barrier function of epithelia. Am. J. Physiol. 241:1981;G275-G288.
32. Heresbach D., Le Gall R., Bretagne J.F., Gosselin M. Etude de la permeabilite intestinale chez l'homme. Gastroenterol. Clin. Biol. 18:1994;638-648.
33. Karlsson J., Artursson P. A method for the determination of cellular permeability coefficients and aquous boundary layer thickness in monolayers of intestinal epithelial (Caco-2) cells grown in permeable filter chambers. Int. J. Pharm. 71:1991;55-64.
34. Dulfer W.J., Groten J.P., Govers A.J. Effect of fatty acids and the aqueous diffusion barrier on the uptake and transport of polychlorinated biphenyls in Caco-2 cells. J. Lipid Res. 37:1996;950-961.
35. Griffiths N.M., Hirst B.H., Simmons N.L. Active intestinal secretion of the fluoroquinolone antibacterials ciprofloxacin, norfloxacin and pefloxacin: a common secretory pathway? J. Pharmacol. Exp. Ther. 269:1994;496-502.
36. Pappenheimer J.R., Reiss K.Z. Contribution of solvent drag through intercellular junctions to absorption of nutrients by the small intestine of the rat. J. Membr. Biol. 100:1987;123-136.
37. Stewart B.H., Chan O.H., Lu R.H., Reyner R.L., Schmid H.L., Hamilton H.W., Steinbaugh B.A., Taylor M.D. Comparison of intestinal permeabilities determined in multiple in vitro and in situ models: relationship to absorption in humans. Pharm. Res. 12:1995;693-699.