MDCK (Madin-Darby canine kidney) cells: A tool for membrane permeability screening

Journal of Pharmaceutical Sciences

Volumn 88, Issue 1, 1999, Pages 28-33

  Irvine, Jennifer D ^a   Takahashi, Lori ^a   Lockhart, Karen ^a   Cheong, Jonathan ^a   Tolan, John W ^a   Selick, H E ^a   Grove, J Russell ^a  

^a AFFYMAX RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLSALICYLIC ACID; ACICLOVIR; ACRIVASTINE; ALPRENOLOL; AMFEBUTAMONE; AMOXICILLIN; ATENOLOL; BRETYLIUM TOSYLATE; CEFALEXIN; CHLOROTHIAZIDE; CORTICOSTERONE; DIMETHYL SULFOXIDE; FLUPAROXAN; FUROSEMIDE; GABAPENTIN; LAMOTRIGINE; LORACARBEF; NETIVUDINE; OLSALAZINE; ONDANSETRON; PARACETAMOL; PHENAZONE; PHENYTOIN; PINDOLOL; PRACTOLOL; SOTALOL; SUMATRIPTAN SUCCINATE; TRIMETHOPRIM; UNINDEXED DRUG; ZIDOVUDINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL STRAIN; DRUG ABSORPTION; HUMAN; HUMAN CELL; INTESTINE ABSORPTION; MEMBRANE; MEMBRANE PERMEABILITY; NONHUMAN;

EID: 0032949615     PISSN: 00223549     EISSN: None     Source Type: Journal    
DOI: 10.1021/js9803205     Document Type: Article

References (34)

1. Pinto, M.; Robine-Leon, S.; Appay, M.-D.; Kedinger, M.; Triadou, N.; Dussaulx, E.; LaCroix, B.; Simon-Assmann, P.; Haffen, K.; Fogh, J.; Zweibaum, A. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol. Cell 1983, 47, 323-330.
2. Hidalgo, I. J.; Raub, T. J.; Borchardt, R. T. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology 1989, 96, 736-749.
3. Wilson, G.; Hassan, I. F.; Dix, C. J.; Williamson, I.; Shah, R.; Mackay, M. Transport and permeability properties of human Caco-2 cells: An in vitro model of the intestinal epithelial cell barrier. J. Controlled Release 1990, 11, 25-40.
4. Artursson, P. Epithelial transport of drugs in cell culture. I: A model for studying the passive diffusion of drugs over intestinal absorbtive (Caco-2) cells. J. Pharm. Sci. 1990, 79, 476-482.
5. Hilgers, A. R.; Conradi, R. A.; Burton, P. S. Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa. Pharm. Res. 1990, 7, 902-910.
6. Artursson, P.; Karlsson, J. Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells. Biochem. Biophys. Res. Comm. 1991, 175, 880-885.
7. Stewart, B. H.; Chan, O. H.; Lu, R. H.; Reyner, E. L.; Schmid, H. L.; Hamilton, H. W.; Steinbaugh, B. A.; Taylor, M. D. Comparison of intestinal permeabilities determined in multiple in vitro and in situ models: relationship to absorption in humans. Pharm. Res. 1995, 12, 693-699.
8. Artursson, P.; Palm, K.; Luthman, K. Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv. Drug Delivery Rev. 1996, 22, 67-84.
9. Hidalgo, I. J. Cultured intestinal epithelial cell models. In Models for Assessing Drug Absorption and Metabolism; Borchardt, R. T., Smith, P. L., Wilson, G., Eds.; Plenum: New York, 1996; pp 35-50.
10. Delie, F.; Rubas, W. A human colonic cell line sharing similarities with enterocytes as a model to examine oral absorption: Advantages and limitations of the Caco-2 model. Crit. Rev. Ther. Drug Carrier Syst. 1997, 14, 221-286.
11. Chong, S. Evaluation of Biocoat intestinal epithelium differentiation environment as an absorption screening model with improved productivity. Pharm. Res. 1996, 13, S-240.
12. Horster, M.; Stopp, M. Transport and metabolic functions in cultured renal tubule cells. Kidney Int. 1986, 29, 46-53.
13. Horio, M.; Chin, K.-V.; Currier, S. J.; Goldenberg, S.; Williams, C.; Pastan, I.; Gottesman, M. M.; Handler, J. Transepithelial transport of drugs by the multidrug transporter in cultured Madin-Darby canine kidney cell epithelia. J. Biol. Chem. 1989, 264, 14880-14884.
14. Hunter, J.; Hirst, B. H.; Simmons, N. L. Transepithelial secretion, cellular accumulation and cytotoxicity of vinblastine in defined MDCK cell strains. Biochim. Biophys. Acta 1993, 1179, 1-10.
15. Hunter, J.; Jepson, M. A.; Tsuruo, T.; Simmons, N. L.; Hirst, B. H. Functional expression of p-glycoprotein in apical membranes of human intestinal Caco-2 cells. Kinetics of vinblastine secretion and interaction with modulators. J. Biol. Chem. 1993, 268, 14991-14997.
16. Brandsch, M.; Ganapathy, V.; Leibach, F. H. H+-peptide contransport in Madin-Darby canine kidney cells: expression and calmodulin-dependent regulation. Am. J. Physiol. 1995, 268, F391-F397.
17. Ganapathy, M. E.; Brandsch, M.; Prasad, P. D.; Ganapathy, V.; Leibach, F. H. Differential recognition of beta-lactam antibiotics by intestinal and renal peptide transporters, PEPT1 and PEPT2. J. Biol. Chem. 1995, 270, 25672-25677.
18. Misfeldt, D. S.; Hamamoto, S. T.; Pitelka, D. R. Transepitheleal transport in cell culture. Proc. Natl. Acad. Sci. U.S.A. 1976, 73, 1212-1216.
19. Cereijido, M.; Bobbins, E. S.; Dolan, W. J.; Rotunno, C. A.; Sabatini, D. D. Polarized monolayers formed by epithelial cells on a permeable and translucent support. J. Cell Biol. 1978, 77, 853-880.
20. Cho, M. J.; Thompson, D. P.; Cramer, C. T.; Vidmar, T. J.; Scieszka, J. F. The Madin-Darby canine kidney (MDCK) epithelial cell monolayer as a model cellular transport barrier. Pharm. Res. 1989, 6, 71-77.
21. Cho, M. J.; Adson, A.; Kezdy, F. J. Transepithelial transport of aliphatic carboxylic acids studied in Madin-Darby canine kidney (MDCK) cell monolayers. Pharm. Res. 1990, 7, 325-331.
22. Ranaldi, G.; Islam, K.; Sambuy, Y. Epithelial cells in culture as a model for the intestinal transport of antimicrobial agents. Antimicrob. Agents Chemother. 1992, 36, 1374-1381.
23. Ranaldi, G.; Seneci, P.; Guba, W.; Islam, K.; Sambuy, Y. Transport of the antibacterial agent oxazolidin-2-one and derivatives across intestinal (Caco-2) and renal (MDCK) epithelial cell lines. Antimicrob. Agents Chemother. 1996, 40, 652-658.
24. Rothen-Rutishauser, B.; Kraemer, S. D.; Braun, A.; Guenthert, M.; Wunderli-Allenspach, H. MDCK cell cultures as an epithelial in vitro model: cytoskeleton and tight junctions as indicators for the definition of age-related stages by confocal microscopy. Pharm. Res. 1998, 15, 964-971.
25. Brown, B. W. Jr.; Hollander, M. Statistics: A Biomedical Introduction; John Wiley & Sons: New York, 1977.
26. Bryan, C. K.; Darby, M. H. Bretylium tosylate: A review. Am. J. Pharm. 1979, 36, 1189-1192.
27. Dressman, J. B.; Amidon, G. L.; Fleisher, D. Absorption potential: estimating the fraction absorbed for orally administered compounds. J. Pharm. Sci. 1985, 74, 588-589.
28. Amidon, G. L.; Sinko, P. J.; Fleisher, D. Estimating human oral fraction dose absorbed: a correlation using rat intestinal membrane permeability for passive and carrier-mediated compounds. Pharm. Res. 1988, 5, 651-654.
29. Gan, L.-S.; Hsyu, P. H.; Pritchard, J. F.; Thakker, D. Mechanism of Intestinal Absorption of Ranitidine and Ondansetron: Transport Across Caco-2 Cell Monolayers. Pharm. Res. 1993, 10, 1722-1725.
30. Rubas, W.; Jezyk, N.; Grass, G. Comparison of the permeability characteristics of a human colonic epithelial (Caco-2) cell line to colon of rabbit, monkey, and dog intestine and human drug absorption. Pharm. Res. 1993, 10, 113-118.
31. Hu, M.; Chen, J.; Zhu, Y.; Dantzig, A. H.; Stratford, R. E.; Kuhfeld, M. T. Mechanism and kinetics of transcellular transport of a new beta-lactam antibiotic loracarbef across an intestinal epithelial membrane model system (Caco-2). Pharm. Res. 1994, 11, 1405-1413.
32. Chong, S.; Dando, S. A.; Soucek, K. M.; Morrison, R. A. In Vitro permeability through Caco-2 cells is not quantitatively predictive of in vivo absorption for peptide-like drugs absorbed via the dipeptide transporter system. Pharm. Res. 1996, 13, 120-123.
33. Wessel, M. D.; Jurs, P. C.; Tolan, J. W.; Muskal, S. Prediction of human intestinal absorption of drug compounds from molecular structure. J. Chem. Info. Comput. Sci. 1998, 38, 726-735.
34. Thwaites, D. T.; Hirst, B. H.; Simmons, N. L. Passive transepithelial absorption of thyrotropin-releasing hormone (TRH) via a paracellular route in cultured intestinal and renal epithelial cell lines. Pharm. Res. 1993, 10, 674-681.