Extremely elevated room-temperature kinetic isotope effects quantify the critical role of barrier width in enzymatic C-H activation

Journal of the American Chemical Society

Volumn 136, Issue 23, 2014, Pages 8157-8160

  Hu, Shenshen ^a,b   Sharma, Sudhir C ^a,b   Scouras, Alexander D ^a,b   Soudackov, Alexander V ^c   Carr, Cody A Marcus ^a,b,d   Hammes Schiffer, Sharon ^c   Alber, Tom ^a,b   Klinman, Judith P ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^d Kiverdi Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; CARRIER MOBILITY; CONFORMATIONS; HYDROGEN; ISOTOPES;

CATALYTIC BEHAVIOR; DONOR-ACCEPTOR DISTANCE; HYDROPHOBIC RESIDUES; KINETIC ISOTOPE EFFECTS; QUANTUM TUNNELING; SITE-SPECIFIC MUTAGENESIS; SOYBEAN LIPOXYGENASE; TEMPERATURE DEPENDENCIES;

KINETICS;

CARBON; ENZYME SOYBEAN LIPOXYGENASE; HYDROGEN; ISOTOPE; LIPOXYGENASE; UNCLASSIFIED DRUG; LINOLEIC ACID;

ARTICLE; CHEMICAL REACTION KINETICS; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME STABILITY; GEL PERMEATION CHROMATOGRAPHY; KINETIC ISOTOPE EFFECT; KINETICS; LIMIT OF QUANTITATION; PROTEIN CONFORMATION; PROTEIN UNFOLDING; QUANTUM THEORY; ROOM TEMPERATURE; SITE DIRECTED MUTAGENESIS; STEADY STATE; TEMPERATURE DEPENDENCE; TURNOVER TIME; ULTRAVIOLET SPECTROSCOPY; X RAY; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; ELECTRON TRANSPORT; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTATION; SOYBEAN; TEMPERATURE;

BINDING SITES; CARBON; CATALYSIS; COMPUTER SIMULATION; ELECTRON TRANSPORT; ENZYME ACTIVATION; HYDROGEN; ISOTOPES; KINETICS; LINOLEIC ACIDS; LIPOXYGENASE; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; QUANTUM THEORY; SOYBEANS; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84902239807     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja502726s     Document Type: Article

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