Modulation of Eg5 activity contributes to mitotic spindle checkpoint activation and Tat-mediated apoptosis in CD4-positive T-lymphocytes

Journal of Pathology

Volumn 233, Issue 2, 2014, Pages 138-147

  Liu, Min ^a   Li, Dengwen ^b   Sun, Lei ^a   Chen, Jie ^b   Sun, Xiaodong ^b   Zhang, Linlin ^b   Huo, Lihong ^b   Zhou, Jun ^b  

^a TIANJIN MEDICAL UNIVERSITY   (China)

^b NANKAI UNIVERSITY   (China)

Author keywords

apoptosis; CD4; Eg5; HIV 1, Tat; mitosis; mitotic spindle checkpoint; T lymphocyte

Indexed keywords

EG5 PROTEIN; LYSINE; MOLECULAR MOTOR; PROTEIN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CD4+ T LYMPHOCYTE; CELL CYCLE PROGRESSION; CONTROLLED STUDY; HUMAN; HUMAN CELL; M PHASE CELL CYCLE CHECKPOINT; MITOSIS; MITOSIS SPINDLE; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN INTERACTION;

APOPTOSIS; CD4; EG5; HIV-1, TAT; MITOSIS; MITOTIC SPINDLE CHECKPOINT; T-LYMPHOCYTE;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALLOSTERIC REGULATION; APOPTOSIS; CD4-POSITIVE T-LYMPHOCYTES; HIV-1; HUMANS; HYDROLYSIS; JURKAT CELLS; KINESIN; LYSINE; M PHASE CELL CYCLE CHECKPOINTS; MITOSIS; PROTEIN BINDING; PROTEIN INTERACTION DOMAINS AND MOTIFS; SIGNAL TRANSDUCTION; SPINDLE APPARATUS; TAT GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; TRANSFECTION;

EID: 84900811585     PISSN: 00223417     EISSN: 10969896     Source Type: Journal    
DOI: 10.1002/path.4333     Document Type: Article

References (39)

1. Meyaard L, Otto S, Jonker R, et al., Programmed death of T cells in HIV-1 infection. Science 1992; 257: 217-219.
2. Romani B, Engelbrecht S, Glashoff RH,. Functions of Tat: the versatile protein of human immunodeficiency virus type 1. J Gen Virol 2010; 91: 1-12.
3. Peruzzi F., The multiple functions of HIV-1 Tat: proliferation versus apoptosis. Front Biosci 2006; 11: 708-717.
4. Pugliese A, Vidotto V, Beltramo T, et al., A review of HIV-1 Tat protein biological effects. Cell Biochem Funct 2005; 23: 223-227.
5. Feng S, Holland EC,. HIV-1 tat trans-activation requires the loop sequence within tar. Nature 1988; 334: 165-167.
6. Berkhout B, Jeang KT,. trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis. J Virol 1989; 63: 5501-5504.
7. Mann DA, Frankel AD,. Endocytosis and targeting of exogenous HIV-1 Tat protein. EMBO J 1991; 10: 1733-1739.
8. Vives E, Brodin P, Lebleu B,. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J Biol Chem 1997; 272: 16010-16017.
9. Debaisieux S, Rayne F, Yezid H, et al., The ins and outs of HIV-1 Tat. Traffic 2012; 13: 355-363.
10. Chen D, Wang M, Zhou S, et al., HIV-1 Tat targets microtubules to induce apoptosis, a process promoted by the pro-apoptotic Bcl-2 relative Bim. EMBO J 2002; 21: 6801-6810.
11. Huo L, Li D, Sun L, et al., Tat acetylation regulates its actions on microtubule dynamics and apoptosis in T lymphocytes. J Pathol 2011a; 223: 28-36.
12. Rath O, Kozielski F,. Kinesins and cancer. Nature Rev Cancer 2012; 12: 527-539.
13. Valentine MT, Gilbert SP,. To step or not to step? How biochemistry and mechanics influence processivity in Kinesin and Eg5. Curr Opin Cell Biol 2007; 19: 75-81.
14. Kapitein LC, Peterman EJ, Kwok BH, et al., The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks. Nature 2005; 435: 114-118.
15. El-Nassan HB,. Advances in the discovery of kinesin spindle protein (Eg5) inhibitors as antitumor agents. Eur J Med Chem 2013; 62: 614-631.
16. Liu M, Wang X, Yang Y, et al., Ectopic expression of the microtubule-dependent motor protein Eg5 promotes pancreatic tumourigenesis. J Pathol 2010; 221: 221-228.
17. He M, Zhang L, Wang X, et al., Systematic analysis of the functions of lysine acetylation in the regulation of Tat activity. PLoS One 2013; 8: e67186.
18. Huo L, Li D, Sun X, et al., Regulation of Tat acetylation and transactivation activity by the microtubule-associated deacetylase HDAC6. J Biol Chem 2011b; 286: 9280-9286.
19. Sun XD, Shi XJ, Sun XO, et al., Dimethylenastron suppresses human pancreatic cancer cell migration and invasion in vitro via allosteric inhibition of mitotic kinesin Eg5. Acta Pharmacol Sin 2011; 32: 1543-1548.
20. Turner J, Anderson R, Guo J, et al., Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker. J Biol Chem 2001; 276: 25496-25502.
21. Bayer P, Kraft M, Ejchart A, et al., Structural studies of HIV-1 tat protein. J Mol Biol 1995; 247: 529-535.
22. Koopman G, Reutelingsperger CP, Kuijten GA, et al., Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 1994; 84: 1415-1420.
23. Zhou J, Yao J, Joshi HC,. Attachment and tension in the spindle assembly checkpoint. J Cell Sci 2002; 115: 3547-3555.
24. Martinou JC, Desagher S, Antonsson B,. Cytochrome c release from mitochondria: all or nothing. Nature Cell Biol 2000; 2: E41-E43.
25. Heeres JT, Hergenrother PJ,. Poly(ADP-ribose) makes a date with death. Curr Opin Chem Biol 2007; 11: 644-653.
26. Battaglia PA, Ponti D, Naim V, et al., The HIV-Tat protein induces chromosome number aberrations by affecting mitosis. Cell Motil Cytoskeleton 2005; 61: 129-136.
27. Hamy F, Felder ER, Heizmann G, et al., An inhibitor of the Tat/TAR RNA interaction that effectively suppresses HIV-1 replication. Proc Natl Acad Sci U S A 1997; 94: 3548-3553.
28. Holmes SC, Arzumanov AA, Gait MJ,. Steric inhibition of human immunodeficiency virus type-1 Tat-dependent trans-activation in vitro and in cells by oligonucleotides containing 2'-O-methyl G-clamp ribonucleoside analogues. Nucleic Acids Res 2003; 31: 2759-2768.
29. Turner JJ, Arzumanov AA, Gait MJ,. Synthesis, cellular uptake and HIV-1 Tat-dependent trans-activation inhibition activity of oligonucleotide analogues disulphide-conjugated to cell-penetrating peptides. Nucleic Acids Res 2005; 33: 27-42.
30. Dormeyer W, Ott M, Schnolzer M,. Probing lysine acetylation in proteins: strategies, limitations, and pitfalls of in vitro acetyltransferase assays. Mol Cell Proteomics 2005; 4: 1226-1239.
31. Hetzer C, Dormeyer W, Schnolzer M, et al., Decoding Tat: the biology of HIV Tat posttranslational modifications. Microbes Infect 2005; 7: 1364-1369.
32. Kiernan RE, Vanhulle C, Schiltz L, et al., HIV-1 tat transcriptional activity is regulated by acetylation. EMBO J 1999; 18: 6106-6118.
33. D'Orso I, Frankel AD,. Tat acetylation modulates assembly of a viral-host RNA-protein transcription complex. Proc Natl Acad Sci U S A 2009; 106: 3101-3106.
34. Ott M, Schnolzer M, Garnica J, et al., Acetylation of the HIV-1 Tat protein by p300 is important for its transcriptional activity. Curr Biol 1999; 9: 1489-1492.
35. Deng L, de la Fuente C, Fu P, et al., Acetylation of HIV-1 Tat by CBP/P300 increases transcription of integrated HIV-1 genome and enhances binding to core histones. Virology 2000; 277: 278-295.
36. Treand C, du Chene I, Bres V, et al., Requirement for SWI/SNF chromatin-remodeling complex in Tat-mediated activation of the HIV-1 promoter. EMBO J 2006; 25: 1690-1699.
37. Mahmoudi T, Parra M, Vries RG, et al., The SWI/SNF chromatin-remodeling complex is a cofactor for Tat transactivation of the HIV promoter. J Biol Chem 2006; 281: 19960-19968.
38. Dorr A, Kiermer V, Pedal A, et al., Transcriptional synergy between Tat and PCAF is dependent on the binding of acetylated Tat to the PCAF bromodomain. EMBO J 2002; 21: 2715-2723.
39. Col E, Caron C, Seigneurin-Berny D, et al., The histone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, Tat. J Biol Chem 2001; 276: 28179-28184.