A 5000-fold increase in the specificity of a bacterial phosphotriesterase for malathion through combinatorial active site mutagenesis

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Naqvi, Tatheer ^a,b   Warden, Andrew C ^b   French, Nigel ^b   Sugrue, Elena ^c   Carr, Paul D ^c   Jackson, Colin J ^c   Scott, Colin ^b  

^a Development Studies   (Pakistan)

^b CSIRO   (Australia)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; MALATHION; PHOSPHOTRIESTERASE; SERINE; TYROSINE; AMINO ACID; INSECTICIDE; PROTEIN BINDING;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME METABOLISM; ENZYME SPECIFICITY; GENE MUTATION; NONHUMAN; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY; TURNOVER TIME; CATALYSIS; CHEMISTRY; COMPUTER PROGRAM; CRYSTALLIZATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE LIBRARY; GENETICS; HYDROLYSIS; KINETICS; MUTAGENESIS; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

AMINO ACIDS; CATALYSIS; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE LIBRARY; HYDROLYSIS; INSECTICIDES; KINETICS; MALATHION; MUTAGENESIS; PHOSPHORIC TRIESTER HYDROLASES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; SOFTWARE; SUBSTRATE SPECIFICITY;

EID: 84899537388     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0094177     Document Type: Article

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