Enhanced production of human full-length immunoglobulin G1 in the periplasm of Escherichia coli

Applied Microbiology and Biotechnology

Volumn 98, Issue 3, 2014, Pages 1237-1246

  Lee, Yong Jae ^a   Lee, Dong Hwa ^a   Jeong, Ki Jun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

5 UTR; Escherichia coli; Fed batch cultivation; Immunoglobulin G; Secretion

Indexed keywords

MAMMALS; MONOCLONAL ANTIBODIES; PURIFICATION;

FED-BATCH CULTIVATION; HIGH-LEVEL PRODUCTION; IMMUNOGLOBULIN G; NEONATAL FC RECEPTORS; OPTIMIZED CONDITIONS; PHARMACEUTICAL PROTEIN; SECRETION; UNTRANSLATED REGION SEQUENCES;

ESCHERICHIA COLI;

ANTHRAX TOXIN; BACTERIAL VECTOR; FC RECEPTOR; IMMUNOGLOBULIN G1; RECOMBINANT PROTEIN; BACTERIAL ANTIGEN; BACTERIAL TOXIN; IMMUNOGLOBULIN G;

ANTIBODY; BIOTECHNOLOGY; COLIFORM BACTERIUM; ECONOMIC ANALYSIS; GENE EXPRESSION; OPTIMIZATION; PROTEIN; SECRETION; TOXIN;

5' UNTRANSLATED REGION; ANTIBODY PRODUCTION; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; BACTERIUM CULTURE; BINDING AFFINITY; BINDING ASSAY; CELL DENSITY; CONTROLLED STUDY; CYTOPLASM; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; FED BATCH CULTURE; GENE EXPRESSION SYSTEM; GLYCOSYLATION; IMMUNOGLOBULIN PRODUCTION; LARGE SCALE PRODUCTION; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; WESTERN BLOTTING; BIOSYNTHESIS; GENETICS; HUMAN; METABOLISM;

ESCHERICHIA COLI; MAMMALIA;

ANTIGENS, BACTERIAL; BACTERIAL TOXINS; ESCHERICHIA COLI; HUMANS; IMMUNOGLOBULIN G; PERIPLASM; RECEPTORS, IGG; RECOMBINANT PROTEINS;

EID: 84898894384     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5390-z     Document Type: Article

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