Evolution of highly active enzymes by homology-independent recombination

Proceedings of the National Academy of Sciences of the United States of America

Volumn 102, Issue 29, 2005, Pages 10082-10087

  Griswold, Karl E ^a   Kawarasaki, Yasuaki ^b,d   Ghoneim, Nada ^b,e   Benkovic, Stephen J ^c   Iverson, Brent L ^a   Georgiou, George ^a,b  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b The University of Texas at Austin   (United States)

^c PENNSYLVANIA STATE UNIVERSITY   (United States)

^d NAGOYA UNIVERSITY   (Japan)

^e UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Enzyme engineering; Enzyme humanization; GST; High throughput screening; ITCHY

Indexed keywords

7 AMINO 4 CHLOROMETHYLCOUMARIN; CHIMERIC PROTEIN; COUMARIN DERIVATIVE; DNA; ETACRYNIC ACID; GLUTATHIONE; GLUTATHIONE TRANSFERASE; UNCLASSIFIED DRUG;

ALPHA HELIX; AMINO ACID SEQUENCE; ARTICLE; COMBINATORIAL CHEMISTRY; CONJUGATION; CROSSING OVER; DNA SHUFFLING; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ENGINEERING; ENZYME ISOLATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; FLOW CYTOMETRY; GENE LIBRARY; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; HYBRID; MOLECULAR EVOLUTION; MOLECULAR RECOGNITION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TECHNIQUE;

ANIMALS; COUMARINS; CROSSING OVER, GENETIC; ETHACRYNIC ACID; FLOW CYTOMETRY; GLUTATHIONE TRANSFERASE; HUMANS; KINETICS; MODELS, MOLECULAR; PEPTIDE LIBRARY; PROTEIN ENGINEERING; PROTEIN HYBRIDIZATION; RATS; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

EID: 22544477815     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0504556102     Document Type: Article

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