High-level production of human leptin by fed-batch cultivation of recombinant Escherichia coli and its purification

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 3027-3032

  Jeong, Ki Jun ^a   Lee, Sang Yup ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

LEPTIN;

ARTICLE; BODY WEIGHT; DENATURATION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; NONHUMAN; OBESITY; PROMOTER REGION; PROTEIN PURIFICATION; PROTEIN SECRETION;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0344242080     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.3027-3032.1999     Document Type: Article

References (27)

1. Altmann, S. W., J. C. Timans, F. L. Rock, J. F. Bazan, and R. A. Kastelein. 1995. Expression and purification of a synthetic human obese gene product. Protein Express. Purif. 6:722-726.
2. Campfield, L. A., F. J. Smith, Y. Guisez, R. Devos, and P. Burn. 1995. Recombinant mouse OB protein: evidence for a peripheral signal linking adiposity and central neutral networks. Science 269:546-549.
3. Cohen, S. L., J. L. Halaas, J. M. Friedman, B. T. Chait, L. Bennett, D. Chang, R. Hecht, and F. Collins. 1996. Human leptin characterization. Nature 382:589.
4. Curless, C., J. Pope, and L. Tsai. 1990. Effect of preinduction specific growth rate on recombinant alpha consensus interferon synthesis in Escherichia coli. Biotechnol. Prog. 6:149-152.
5. Fass, R., M. van de Walle, A. Shiloach, A. Joslyn, J. Kaufman, and J. Shiloach. 1991. Use of high density cultures of Escherichia coli for high level production of recombinant Pseudomonos aeruginosa exotoxin A. Appl. Microbiol. Biotechnol. 36:65-69.
6. Fawzi, A. B., H. Zhang, M. van Heek, and M. P. Graziano. 1996. Purification of milligram quantities of human leptin from recombinant E. coli. Horm. Metab. Res. 28:694-697.
7. Froguel, P., and J. Hager. 1995. Human diabetes and obesity: tracking down the genes. Trends Biotechnol. 13:52-55.
8. Giese, K., W. J. Fantl, C. Vitt, J. C. Stephans, L. Consens, M. Wachowicz, and L. T. Williams. 1996. Reduction of food intake and weight gain by the ob protein requires a specific secondary structure and is reversible. Mol. Med. 2:50-58.
9. Halaas, J. L., K. S. Gajiwala, M. Maffei, S. L. Cohen, B. T. Chait, D. Rabinowitz, R L. Lallone, S. K. Burley, and J. M. Friedman. 1995. Weight-reducing effects of the plasma protein encoded by the ob gene. Science 269:543-546.
10. Hellmuth, K., D. J. Korz, E. A. Sanders, and W. D. Deckwer. 1994. Effect of growth rate on stability and gene expression of recombinant plasmids during continuous and high cell density cultivation of Escherichia coli TG1. J. Biotechnol. 32:289-298.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
12. Lee, S. Y. 1996. High cell-density culture of Escherichia coli. Trends Biotechnol. 14:98-105.
13. Lee, S. Y., and H. N. Chang. 1993. High cell density cultivation of Escherichia coli W using sucrose as a carbon source. Biotechnol. Lett. 15:971-974.
14. Lee, S. Y., and H. N. Chang. 1994. Effect of complex nitrogen source on the synthesis and accumulation of poly(3-hydroxybutyric acid) by recombinant Escherichia coli in flask and fed-batch cultures. J. Environ. Polym. Degrad. 2:169-176.
15. Matsumae, H., S. Minobe, K. Kindan, T. Watanabe, T. Sato, and T. Tosa. 1990. Specific removal of endotoxin from protein solutions by immobilized histidine. Biotechnol. Appl. Biochem. 12:129-140.
16. Morsy, M. A., M. C. Gu, J. Z. Zhao, D. J. Holder, I. T. Rogers, W. J. Pouch, S. L. Motzel, H. J. Klein, S. K. Gupta, X. Liang, M. R. Tota, C. I. Rosenblum, and C. T. Caskey. 1998. Leptin gene therapy and daily protein administration: a comparative study in the ob/ob mouse. Gene Ther. 5:8-18.
17. Pelleymounter, M. A., M. J. Cullen, M. B. Baker, R. Hecht, D. Winters, T. Boone, and F. Collins. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269:540-543.
18. Rentsch, J., N. Levens, and M. Chiesi. 1995. Recombinant ob-gene product reduces food intake in fasted mice. Biochem. Biophys. Res. Commun. 214: 131-136.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Shin, C. S., M. S. Hong, C. S. Bae, and J. Lee. 1997. Enhanced production of human mini-proinsulin in fed-batch cultures at high cell density of Escherichia coli BL21(DE3)[pET-3aT2M2]. Biotechnol. Prog. 13:249-257.
21. Suzuki, T., T. Yamane, and S. Shimizu. 1990. Phenomenological background and some preliminary trials of automated substrate supply in pH-stat modal fed-batch culture using a setpoint of high limit. J. Ferment. Bioeng. 69:292-297.
22. Sweadner, K. J., M. Forte, and L. L. Nelsen. 1977. Filtration removal of endotoxin (pyrogen) in solution in different stages of aggregation. Appl. Environ. Microbiol. 34:382-385.
23. Varnerin, J. P., T. Smith, C. I. Rosenblum, A. Vongs, B. A. Murphy, C. Nunes, T. N. Mellin, J. J. King, B. W. Burgess, B. Junker, M. Chou, P. Hey, E. Frazier, D. E. MacIntyre, L. H. T. van der Ploeg, and M. R. Tota. 1998. Production of leptin in Escherichia coli: a comparison of methods. Protein Expr. Purif. 14:335-342.
24. Weigle, D. S., T. R. Bukowski, D. C. Foster, S. Holderman, J. M. Kramer, G. Lasser, C. E. Lofton-Day, D. E. Prunkard, C. Raymond, and J. L. Kuijper. 1995. Recombinant ob protein reduces feeding and body weight in the ob/ob mouse. J. Clin. Invest. 96:2065-2070.
25. Wong, H. H., Y. C. Kim, S. Y. Lee, and H. N. Chang. 1998. Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli. Biotechnol. Bioeng. 60:271-276.
26. Zamorano, P. L., L. De Sevilla, V. B. Mahesh, and D. W. Brann. 1997. Production and refolding of recombinant leptin. BioTechniques 23:800-804.
27. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold, and J. M. Friedman. 1994. Positional cloning of the mouse ob gene and its human homologue. Nature 372:425-432.